ID   BAFA_XENLA              Reviewed;          90 AA.
AC   Q6NTS2; A3KNC4;
DT   21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Barrier-to-autointegration factor A;
GN   Name=banf1-a; Synonyms=baf-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg, and Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PHOSPHORYLATION.
RX   PubMed=16371512; DOI=10.1091/mbc.e05-04-0356;
RA   Bengtsson L., Wilson K.L.;
RT   "Barrier-to-autointegration factor phosphorylation on Ser-4 regulates
RT   emerin binding to lamin A in vitro and emerin localization in vivo.";
RL   Mol. Biol. Cell 17:1154-1163(2006).
RN   [3]
RP   SUBCELLULAR LOCATION, INTERACTION WITH NEMP1A AND NEMP1B, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=19167377; DOI=10.1016/j.ydbio.2008.12.038;
RA   Mamada H., Takahashi N., Taira M.;
RT   "Involvement of an inner nuclear membrane protein, Nemp1, in Xenopus neural
RT   development through an interaction with the chromatin protein BAF.";
RL   Dev. Biol. 327:497-507(2009).
CC   -!- FUNCTION: Plays fundamental roles in nuclear assembly, chromatin
CC       organization, gene expression and gonad development. May potently
CC       compress chromatin structure and be involved in membrane recruitment
CC       and chromatin decondensation during nuclear assembly. Contains 2 non-
CC       specific dsDNA-binding sites which may promote DNA cross-bridging (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. Binds non-specifically to double-stranded DNA, and
CC       is found as hexamer or dodecamer upon DNA binding. Binds to LEM domain-
CC       containing nuclear proteins (By similarity). Interacts with nemp1a and
CC       nemp1b. {ECO:0000250, ECO:0000269|PubMed:19167377}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19167377}. Nucleus
CC       envelope {ECO:0000269|PubMed:19167377}. Chromosome {ECO:0000250}.
CC       Note=Colocalizes with nemp1a and nemp1b at the nuclear envelope.
CC       {ECO:0000269|PubMed:19167377}.
CC   -!- DEVELOPMENTAL STAGE: At the early gastrula stage, expressed mainly in
CC       the entire animal hemisphere. During neurulation, its expression
CC       becomes restricted to the anterior neuroectoderm. At the tailbud stage,
CC       expressed in various anterior regions including the anterior central
CC       nervous system (CNS), otic vesicles, and branchial arches.
CC       {ECO:0000269|PubMed:19167377}.
CC   -!- PTM: Phosphorylated during S and M phases.
CC       {ECO:0000269|PubMed:16371512}.
CC   -!- SIMILARITY: Belongs to the BAF family. {ECO:0000305}.
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DR   EMBL; BC068883; AAH68883.1; -; mRNA.
DR   EMBL; BC133759; AAI33760.1; -; mRNA.
DR   RefSeq; NP_001084558.1; NM_001091089.1.
DR   AlphaFoldDB; Q6NTS2; -.
DR   SMR; Q6NTS2; -.
DR   DNASU; 414508; -.
DR   GeneID; 414508; -.
DR   KEGG; xla:414508; -.
DR   CTD; 414508; -.
DR   Xenbase; XB-GENE-6078848; banf1.L.
DR   OMA; EDLFWEW; -.
DR   OrthoDB; 1617480at2759; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 414508; Expressed in gastrula and 19 other tissues.
DR   GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0005635; C:nuclear envelope; IDA:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.150.40; -; 1.
DR   InterPro; IPR004122; BAF_prot.
DR   InterPro; IPR036617; BAF_sf.
DR   Pfam; PF02961; BAF; 1.
DR   SMART; SM01023; BAF; 1.
DR   SUPFAM; SSF47798; SSF47798; 1.
PE   1: Evidence at protein level;
KW   Chromosome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..90
FT                   /note="Barrier-to-autointegration factor A"
FT                   /id="PRO_0000223614"
SQ   SEQUENCE   90 AA;  10206 MW;  C230A7184744060B CRC64;
     MSSTSQKHRD FVAEPMGEKS VQCLAGIGEA LGHRLEEKGF DKAYVVLGQF LVLKKDEELF
     KEWLKDICSA NAKQSRDCYG CLKEWCDAFL