BAF_DROME
ID BAF_DROME Reviewed; 90 AA.
AC Q9VLU0; Q53XE9;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Barrier-to-autointegration factor;
GN Name=baf; ORFNames=CG7380;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=12902403; DOI=10.1242/jcs.00682;
RA Furukawa K., Sugiyama S., Osouda S., Goto H., Inagaki M., Horigome T.,
RA Omata S., McConnell M., Fisher P.A., Nishida Y.;
RT "Barrier-to-autointegration factor plays crucial roles in cell cycle
RT progression and nuclear organization in Drosophila.";
RL J. Cell Sci. 116:3811-3823(2003).
RN [5]
RP INTERACTION WITH MAD1.
RX PubMed=20036230; DOI=10.1016/j.ydbio.2009.11.036;
RA Wagner N., Weyhersmueller A., Blauth A., Schuhmann T., Heckmann M.,
RA Krohne G., Samakovlis C.;
RT "The Drosophila LEM-domain protein MAN1 antagonizes BMP signaling at the
RT neuromuscular junction and the wing crossveins.";
RL Dev. Biol. 339:1-13(2010).
CC -!- FUNCTION: Plays fundamental roles in nuclear assembly, chromatin
CC organization, gene expression and gonad development. May potently
CC compress chromatin structure and be involved in membrane recruitment
CC and chromatin decondensation during nuclear assembly. Functions are
CC required in both M phase and interphase of the cell cycle.
CC {ECO:0000269|PubMed:12902403}.
CC -!- SUBUNIT: May interact with MAD1. {ECO:0000269|PubMed:20036230}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12902403}. Cytoplasm
CC {ECO:0000269|PubMed:12902403}. Chromosome
CC {ECO:0000269|PubMed:12902403}. Note=Colocalizes with chromosomes during
CC both interphase and mitosis.
CC -!- DISRUPTION PHENOTYPE: Death at the larval-pupal transition with small
CC brains and missing imaginal disks. Specific abnormalities in interphase
CC nuclear structure; clumping of chromatin and convolution of nuclear
CC shape. {ECO:0000269|PubMed:12902403}.
CC -!- SIMILARITY: Belongs to the BAF family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52592.1; -; Genomic_DNA.
DR EMBL; BT011552; AAS15688.1; -; mRNA.
DR RefSeq; NP_001260220.1; NM_001273291.1.
DR RefSeq; NP_609176.1; NM_135332.4.
DR AlphaFoldDB; Q9VLU0; -.
DR SMR; Q9VLU0; -.
DR BioGRID; 60229; 13.
DR DIP; DIP-23961N; -.
DR IntAct; Q9VLU0; 2.
DR STRING; 7227.FBpp0305308; -.
DR iPTMnet; Q9VLU0; -.
DR PaxDb; Q9VLU0; -.
DR PRIDE; Q9VLU0; -.
DR EnsemblMetazoa; FBtr0079535; FBpp0079157; FBgn0031977.
DR EnsemblMetazoa; FBtr0333095; FBpp0305308; FBgn0031977.
DR GeneID; 34095; -.
DR KEGG; dme:Dmel_CG7380; -.
DR CTD; 12016; -.
DR FlyBase; FBgn0031977; baf.
DR VEuPathDB; VectorBase:FBgn0031977; -.
DR eggNOG; KOG4233; Eukaryota.
DR GeneTree; ENSGT00940000167655; -.
DR HOGENOM; CLU_167806_0_0_1; -.
DR InParanoid; Q9VLU0; -.
DR OMA; SKQQGDC; -.
DR OrthoDB; 1617480at2759; -.
DR PhylomeDB; Q9VLU0; -.
DR SignaLink; Q9VLU0; -.
DR BioGRID-ORCS; 34095; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 34095; -.
DR PRO; PR:Q9VLU0; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031977; Expressed in eye disc (Drosophila) and 22 other tissues.
DR ExpressionAtlas; Q9VLU0; baseline and differential.
DR Genevisible; Q9VLU0; DM.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0030261; P:chromosome condensation; IMP:FlyBase.
DR GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR GO; GO:0030717; P:oocyte karyosome formation; IMP:FlyBase.
DR Gene3D; 1.10.150.40; -; 1.
DR InterPro; IPR004122; BAF_prot.
DR InterPro; IPR036617; BAF_sf.
DR Pfam; PF02961; BAF; 1.
DR SMART; SM01023; BAF; 1.
DR SUPFAM; SSF47798; SSF47798; 1.
PE 1: Evidence at protein level;
KW Chromosome; Cytoplasm; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..90
FT /note="Barrier-to-autointegration factor"
FT /id="PRO_0000221032"
SQ SEQUENCE 90 AA; 10116 MW; 5BCB777FD3BE000E CRC64;
MSGTSQKHRN FVAEPMGNKS VTELAGIGET LGGRLKDAGF DMAYTVLGQY LVLKKDEELF
KDWMKEVCHA SSKQASDCYN CLNDWCEEFL
