BAG3_ARATH
ID BAG3_ARATH Reviewed; 303 AA.
AC Q9LYP4; Q8LFF2;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=BAG family molecular chaperone regulator 3;
DE AltName: Full=Bcl-2-associated athanogene 3;
GN Name=BAG3; OrderedLocusNames=At5g07220; ORFNames=T28J14_160;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX DOI=10.1016/S0168-9452(03)00121-3;
RA Juqiang Y., Cixin H., Hong Z.;
RT "The BAG-family proteins in Arabidopsis thaliana.";
RL Plant Sci. 165:1-7(2003).
RN [6]
RP INTERACTION WITH HSP70-1.
RX PubMed=16003391; DOI=10.1038/sj.cdd.4401712;
RA Kang C.H., Jung W.Y., Kang Y.H., Kim J.Y., Kim D.G., Jeong J.C., Baek D.W.,
RA Jin J.B., Lee J.Y., Kim M.O., Chung W.S., Mengiste T., Koiwa H., Kwak S.S.,
RA Bahk J.D., Lee S.Y., Nam J.S., Yun D.J., Cho M.J.;
RT "AtBAG6, a novel calmodulin-binding protein, induces programmed cell death
RT in yeast and plants.";
RL Cell Death Differ. 13:84-95(2006).
RN [7]
RP GENE FAMILY.
RX PubMed=16636050; DOI=10.1074/jbc.m511794200;
RA Doukhanina E.V., Chen S., van der Zalm E., Godzik A., Reed J.,
RA Dickman M.B.;
RT "Identification and functional characterization of the BAG protein family
RT in Arabidopsis thaliana.";
RL J. Biol. Chem. 281:18793-18801(2006).
CC -!- FUNCTION: Co-chaperone that regulates diverse cellular pathways, such
CC as programmed cell death and stress responses. {ECO:0000250}.
CC -!- SUBUNIT: Binds to the ATPase domain of HSP70/HSC70 chaperones (By
CC similarity). Interacts with HSP70-1. {ECO:0000250,
CC ECO:0000269|PubMed:16003391}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL163652; CAB87278.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91123.1; -; Genomic_DNA.
DR EMBL; AF428411; AAL16179.1; -; mRNA.
DR EMBL; AY084885; AAM61448.1; -; mRNA.
DR PIR; T48493; T48493.
DR RefSeq; NP_196339.1; NM_120804.3.
DR PDB; 4HWF; X-ray; 2.00 A; A/B=135-220.
DR PDBsum; 4HWF; -.
DR AlphaFoldDB; Q9LYP4; -.
DR SMR; Q9LYP4; -.
DR IntAct; Q9LYP4; 1.
DR STRING; 3702.AT5G07220.1; -.
DR PaxDb; Q9LYP4; -.
DR PRIDE; Q9LYP4; -.
DR ProteomicsDB; 240845; -.
DR EnsemblPlants; AT5G07220.1; AT5G07220.1; AT5G07220.
DR GeneID; 830613; -.
DR Gramene; AT5G07220.1; AT5G07220.1; AT5G07220.
DR KEGG; ath:AT5G07220; -.
DR Araport; AT5G07220; -.
DR TAIR; locus:2182900; AT5G07220.
DR eggNOG; KOG4361; Eukaryota.
DR HOGENOM; CLU_043370_1_0_1; -.
DR InParanoid; Q9LYP4; -.
DR OMA; QTASWET; -.
DR OrthoDB; 1266506at2759; -.
DR PhylomeDB; Q9LYP4; -.
DR PRO; PR:Q9LYP4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LYP4; baseline and differential.
DR Genevisible; Q9LYP4; AT.
DR GO; GO:0000774; F:adenyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0051087; F:chaperone binding; IBA:GO_Central.
DR GO; GO:0050821; P:protein stabilization; IBA:GO_Central.
DR Gene3D; 1.20.58.120; -; 1.
DR InterPro; IPR039773; BAG_chaperone_regulator.
DR InterPro; IPR036533; BAG_dom_sf.
DR InterPro; IPR003103; BAG_domain.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR12329; PTHR12329; 1.
DR Pfam; PF02179; BAG; 1.
DR SMART; SM00264; BAG; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR SUPFAM; SSF63491; SSF63491; 1.
DR PROSITE; PS51035; BAG; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chaperone; Phosphoprotein; Reference proteome.
FT CHAIN 1..303
FT /note="BAG family molecular chaperone regulator 3"
FT /id="PRO_0000415523"
FT DOMAIN 45..119
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00214"
FT DOMAIN 138..216
FT /note="BAG"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00369"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 249..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 263
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q0WUQ1"
FT CONFLICT 45
FT /note="F -> I (in Ref. 4; AAM61448)"
FT /evidence="ECO:0000305"
FT HELIX 135..161
FT /evidence="ECO:0007829|PDB:4HWF"
FT HELIX 168..189
FT /evidence="ECO:0007829|PDB:4HWF"
FT TURN 190..192
FT /evidence="ECO:0007829|PDB:4HWF"
FT HELIX 194..218
FT /evidence="ECO:0007829|PDB:4HWF"
SQ SEQUENCE 303 AA; 34244 MW; EB3464BD401A9D37 CRC64;
MMKMNTGTSP SVIGGGTSGN EWESRPGGMV VQRRTDQNSD VPRVFRVRVK YGSVYHEINI
NSQSSFGELK KMLSDQVGLH HEDMKVLYKD KERDSKMFLD LCGVKDRSKL VVKEDPISQE
KRLLAKRKNA AIEKASKSIS DISFEVDRLA GQVSAFETVI NKGGKVEEKS LVNLIEMLMN
QLLRLDAIIA DGDVKLMRKM QVQRVQKYVE ALDLLKVKNS AKKVEVNKSV RHKPQTQTRF
EQRDLLSFVE EEEEEPRNSN ASSSSGTPAV VASKWEMFDS ASTAKAAETV KPVPPRFKWE
FFD
