BAM9_ARATH
ID BAM9_ARATH Reviewed; 536 AA.
AC Q8VYW2; Q946D4;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Inactive beta-amylase 9;
DE AltName: Full=1,4-alpha-D-glucan maltohydrolase;
DE AltName: Full=Inactive beta-amylase 3;
GN Name=BAM9; Synonyms=BMY3; OrderedLocusNames=At5g18670; ORFNames=T1A4.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Landsberg erecta;
RX DOI=10.1016/S0168-9452(01)00508-8;
RA Chandler J.W., Apel K., Melzer S.;
RT "A novel putative beta-amylase gene and ATbeta-Amy from Arabidopsis
RT thaliana are circadian regulated.";
RL Plant Sci. 161:1019-1024(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18390594; DOI=10.1105/tpc.107.056507;
RA Fulton D.C., Stettler M., Mettler T., Vaughan C.K., Li J., Francisco P.,
RA Gil M., Reinhold H., Eicke S., Messerli G., Dorken G., Halliday K.,
RA Smith A.M., Smith S.M., Zeeman S.C.;
RT "Beta-AMYLASE4, a noncatalytic protein required for starch breakdown, acts
RT upstream of three active beta-amylases in Arabidopsis chloroplasts.";
RL Plant Cell 20:1040-1058(2008).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in young floral buds, flowers and
CC roots, and, to a later extent, in stems and leaves.
CC {ECO:0000269|Ref.1}.
CC -!- INDUCTION: Circadian-regulated, with a peak in expression just before
CC the light period in short day conditions. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 14 family. {ECO:0000305}.
CC -!- CAUTION: In contrast to other members of the family, lacks the
CC conserved Glu active site in position 449, which is replaced by a Gln
CC residue, suggesting it is inactive. {ECO:0000305}.
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DR EMBL; AF402598; AAK85300.1; -; mRNA.
DR EMBL; AC051627; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92597.1; -; Genomic_DNA.
DR EMBL; AY069879; AAL47434.1; -; mRNA.
DR EMBL; AY142007; AAM98271.1; -; mRNA.
DR EMBL; AY087036; AAM64597.1; -; mRNA.
DR RefSeq; NP_197368.1; NM_121872.3.
DR AlphaFoldDB; Q8VYW2; -.
DR SMR; Q8VYW2; -.
DR STRING; 3702.AT5G18670.1; -.
DR CAZy; GH14; Glycoside Hydrolase Family 14.
DR PaxDb; Q8VYW2; -.
DR PRIDE; Q8VYW2; -.
DR ProteomicsDB; 240708; -.
DR EnsemblPlants; AT5G18670.1; AT5G18670.1; AT5G18670.
DR GeneID; 831985; -.
DR Gramene; AT5G18670.1; AT5G18670.1; AT5G18670.
DR KEGG; ath:AT5G18670; -.
DR Araport; AT5G18670; -.
DR TAIR; locus:2180029; AT5G18670.
DR eggNOG; ENOG502QPTU; Eukaryota.
DR HOGENOM; CLU_016754_5_0_1; -.
DR InParanoid; Q8VYW2; -.
DR OMA; RFSWAGY; -.
DR OrthoDB; 533202at2759; -.
DR PhylomeDB; Q8VYW2; -.
DR PRO; PR:Q8VYW2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8VYW2; baseline and differential.
DR Genevisible; Q8VYW2; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016161; F:beta-amylase activity; IEA:InterPro.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR InterPro; IPR001554; Glyco_hydro_14.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR31352; PTHR31352; 1.
DR Pfam; PF01373; Glyco_hydro_14; 1.
DR PRINTS; PR00750; BETAAMYLASE.
DR SUPFAM; SSF51445; SSF51445; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Cytoplasm; Phosphoprotein;
KW Polysaccharide degradation; Reference proteome.
FT CHAIN 1..536
FT /note="Inactive beta-amylase 9"
FT /id="PRO_0000393423"
FT REGION 511..536
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 47
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LIR6"
FT CONFLICT 69..70
FT /note="DD -> GA (in Ref. 1; AAK85300)"
FT /evidence="ECO:0000305"
FT CONFLICT 442..443
FT /note="QG -> RKA (in Ref. 1; AAK85300)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 536 AA; 58281 MW; C21F0582BE72E190 CRC64;
MEVSVIGNPQ ARICRAELAY RELGFRFGSD VISGESRNRV SFCNQSSKWK EIAIRCSSRS
VKCEAIVSDD ASPFLKSTPK SKSLESVKLF VGLPLDTVSD CNNVNHLKAI TAGLKALKLL
GVEGIELPIF WGVVEKEAAG KYEWSGYLAV AEIVKKVGLK LHASLSFHGS KQTEIGLPDW
VAKIGDAEPG IYFTDRYGQQ YKDCLSFAVD DVPVLDGKTP MEVYRGFCES FKSAFADYMG
NTITGITLGL GPDGELKYPS HQHNAKLSGA GEFQCYDKHM LSALKGYAES TGNPLWGLGG
PHDAPAYDQQ PNSSSFFSDG GSWESQYGDF FLSWYSSLLT SHADRVLSVA SSAFSGIGVP
LCGKLPLLHQ WHKLRSHPSE LTAGFYSSNG QDRYEAIAEI FAKNSCRMII PGMDLSDEHQ
SPESLSSPES LLGHIKTSCK KQGVVVSGQN SSTPVPGGFE RIVENLKDEN VGIDLFTYQR
MGALFFSPEH FHAFTVFVRN LSQFELSSDD QASEAEVEAE TASIGSGTGA PSLQTA
