BAMA2_AMAPH
ID BAMA2_AMAPH Reviewed; 32 AA.
AC A8W7P1;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 25-MAY-2022, entry version 15.
DE RecName: Full=Beta-amanitin proprotein {ECO:0000303|PubMed:18025465};
DE Contains:
DE RecName: Full=Beta-amanitin {ECO:0000303|PubMed:18025465};
DE Flags: Precursor; Fragment;
GN Name=AMA2 {ECO:0000303|PubMed:18025465};
OS Amanita phalloides (Death cap).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Amanitaceae; Amanita.
OX NCBI_TaxID=67723;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=18025465; DOI=10.1073/pnas.0707340104;
RA Hallen H.E., Luo H., Scott-Craig J.S., Walton J.D.;
RT "Gene family encoding the major toxins of lethal Amanita mushrooms.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19097-19101(2007).
RN [2]
RP REVIEW ON TOXICITY.
RX PubMed=12475187; DOI=10.1081/clt-120014646;
RA Enjalbert F., Rapior S., Nouguier-Soule J., Guillon S., Amouroux N.,
RA Cabot C.;
RT "Treatment of amatoxin poisoning: 20-year retrospective analysis.";
RL J. Toxicol. Clin. Toxicol. 40:715-757(2002).
CC -!- FUNCTION: Toxin belonging to the bicyclic octapeptides amatoxins that
CC acts by binding non-competitively to RNA polymerase II and greatly
CC slowing the elongation of transcripts from target promoters
CC (PubMed:18025465). {ECO:0000305|PubMed:18025465}.
CC -!- PTM: Processed by the macrocyclase-peptidase enzyme POPB to yield a
CC toxic cyclic decapeptide (PubMed:18025465). POPB first removes 10
CC residues from the N-terminus (By similarity). Conformational trapping
CC of the remaining peptide forces the enzyme to release this intermediate
CC rather than proceed to macrocyclization (By similarity). The enzyme
CC rebinds the remaining peptide in a different conformation and catalyzes
CC macrocyclization of the N-terminal 8 residues (By similarity).
CC {ECO:0000250|UniProtKB:A0A067SLB9, ECO:0000305|PubMed:18025465}.
CC -!- MISCELLANEOUS: The typical symptoms of amatoxin poisoning are gastro-
CC intestinal distress beginning 6-12 hours after ingestion, a remission
CC phase lasting 12-24 hours, and progressive loss of liver function
CC culminating in death within 3-5 days (PubMed:12475187). One of the few
CC effective treatments is liver transplantation (PubMed:12475187).
CC {ECO:0000303|PubMed:12475187}.
CC -!- SIMILARITY: Belongs to the MSDIN fungal toxin family. {ECO:0000305}.
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DR EMBL; EU196156; ABW87785.1; -; Genomic_DNA.
DR AlphaFoldDB; A8W7P1; -.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR027582; Amanitin/phalloidin.
DR TIGRFAMs; TIGR04309; amanitin; 1.
PE 3: Inferred from homology;
KW Thioether bond; Toxin.
FT PROPEP 1..10
FT /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT /id="PRO_0000443590"
FT PEPTIDE 11..18
FT /note="Beta-amanitin"
FT /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT /id="PRO_0000443591"
FT PROPEP 19..32
FT /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT /id="PRO_0000443592"
FT CROSSLNK 11..18
FT /note="Cyclopeptide (Ile-Pro)"
FT /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT CROSSLNK 12..16
FT /note="2'-cysteinyl-6'-hydroxytryptophan sulfoxide (Trp-
FT Cys)"
FT /evidence="ECO:0000250|UniProtKB:P85421"
FT NON_TER 32
FT /evidence="ECO:0000305"
SQ SEQUENCE 32 AA; 3446 MW; 6AE1D3A7DC60C699 CRC64;
MSDINATRLP IWGIGCDPCI GDDVTILLTR GE
