ID   BAMA3_AMAPH             Reviewed;          36 AA.
AC   D6CFW3;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   13-JUL-2010, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Beta-amanitin proprotein {ECO:0000303|Ref.1};
DE   Contains:
DE     RecName: Full=Beta-amanitin {ECO:0000303|Ref.1};
DE   Flags: Precursor;
OS   Amanita phalloides (Death cap).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Amanitaceae; Amanita.
OX   NCBI_TaxID=67723;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Epis S., Matinato C., Gentili G., Varotto F., Bandi C., Sassera D.;
RT   "Molecular method for assessing the presence of alpha amanitin in the genus
RT   Amanita.";
RL   Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   REVIEW ON TOXICITY.
RX   PubMed=12475187; DOI=10.1081/clt-120014646;
RA   Enjalbert F., Rapior S., Nouguier-Soule J., Guillon S., Amouroux N.,
RA   Cabot C.;
RT   "Treatment of amatoxin poisoning: 20-year retrospective analysis.";
RL   J. Toxicol. Clin. Toxicol. 40:715-757(2002).
CC   -!- FUNCTION: Toxin belonging to the bicyclic octapeptides amatoxins that
CC       acts by binding non-competitively to RNA polymerase II and greatly
CC       slowing the elongation of transcripts from target promoters (By
CC       similarity). {ECO:0000250|UniProtKB:A0A023IWK6}.
CC   -!- PTM: Processed by the macrocyclase-peptidase enzyme POPB to yield a
CC       toxic cyclic decapeptide (By similarity). POPB first removes 10
CC       residues from the N-terminus (By similarity). Conformational trapping
CC       of the remaining peptide forces the enzyme to release this intermediate
CC       rather than proceed to macrocyclization (By similarity). The enzyme
CC       rebinds the remaining peptide in a different conformation and catalyzes
CC       macrocyclization of the N-terminal 8 residues (By similarity).
CC       {ECO:0000250|UniProtKB:A0A067SLB9}.
CC   -!- MISCELLANEOUS: The typical symptoms of amatoxin poisoning are gastro-
CC       intestinal distress beginning 6-12 hours after ingestion, a remission
CC       phase lasting 12-24 hours, and progressive loss of liver function
CC       culminating in death within 3-5 days (PubMed:12475187). One of the few
CC       effective treatments is liver transplantation (PubMed:12475187).
CC       {ECO:0000303|PubMed:12475187}.
CC   -!- SIMILARITY: Belongs to the MSDIN fungal toxin family. {ECO:0000305}.
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DR   EMBL; FN555142; CBG76461.1; -; Genomic_DNA.
DR   AlphaFoldDB; D6CFW3; -.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR027582; Amanitin/phalloidin.
DR   TIGRFAMs; TIGR04309; amanitin; 1.
PE   3: Inferred from homology;
KW   Thioether bond; Toxin.
FT   PROPEP          1..10
FT                   /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT                   /id="PRO_0000443593"
FT   PEPTIDE         11..18
FT                   /note="Beta-amanitin"
FT                   /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT                   /id="PRO_0000443594"
FT   PROPEP          19..36
FT                   /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT                   /id="PRO_0000443595"
FT   CROSSLNK        11..18
FT                   /note="Cyclopeptide (Ile-Pro)"
FT                   /evidence="ECO:0000250|UniProtKB:A8W7M4"
FT   CROSSLNK        12..16
FT                   /note="2'-cysteinyl-6'-hydroxytryptophan sulfoxide (Trp-
FT                   Cys)"
FT                   /evidence="ECO:0000250|UniProtKB:P85421"
SQ   SEQUENCE   36 AA;  3778 MW;  CAAD8EFAEAE1D3A7 CRC64;
     MSDINATRLP IWGIGCDPCI GDDVTALLTR GEASLC