ID   RS4_ECOL6               Reviewed;         206 AA.
AC   P0A7V9; P02354;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=30S ribosomal protein S4;
GN   Name=rpsD; Synonyms=ramA; OrderedLocusNames=c4057;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: One of two assembly initiator proteins for the 30S subunit,
CC       it binds directly to 16S rRNA where it nucleates assembly of the body
CC       of the 30S subunit. {ECO:0000250}.
CC   -!- FUNCTION: With S5 and S12 plays an important role in translational
CC       accuracy; many suppressors of streptomycin-dependent mutants of protein
CC       S12 are found in this protein, some but not all of which decrease
CC       translational accuracy (ram, ribosomal ambiguity mutations).
CC       {ECO:0000250}.
CC   -!- FUNCTION: Protein S4 is also a translational repressor protein, it
CC       controls the translation of the alpha-operon (which codes for S13, S11,
CC       S4, RNA polymerase alpha subunit, and L17) by binding to its mRNA.
CC       {ECO:0000250}.
CC   -!- FUNCTION: Also functions as a rho-dependent antiterminator of rRNA
CC       transcription, increasing the synthesis of rRNA under conditions of
CC       excess protein, allowing a more rapid return to homeostasis. Binds
CC       directly to RNA polymerase (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts protein S5. Some
CC       nascent polypeptide chains are able to cross-link to this protein in
CC       situ (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the universal ribosomal protein uS4 family.
CC       {ECO:0000305}.
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DR   EMBL; AE014075; AAN82495.1; -; Genomic_DNA.
DR   RefSeq; WP_000135224.1; NC_004431.1.
DR   AlphaFoldDB; P0A7V9; -.
DR   SMR; P0A7V9; -.
DR   STRING; 199310.c4057; -.
DR   EnsemblBacteria; AAN82495; AAN82495; c4057.
DR   GeneID; 67415337; -.
DR   KEGG; ecc:c4057; -.
DR   eggNOG; COG0522; Bacteria.
DR   HOGENOM; CLU_092403_0_2_6; -.
DR   OMA; NVVFRMG; -.
DR   BioCyc; ECOL199310:C4057-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
DR   GO; GO:0006353; P:DNA-templated transcription, termination; IEA:UniProtKB-KW.
DR   GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.10.290.10; -; 1.
DR   HAMAP; MF_01306_B; Ribosomal_S4_B; 1.
DR   InterPro; IPR022801; Ribosomal_S4/S9.
DR   InterPro; IPR001912; Ribosomal_S4/S9_N.
DR   InterPro; IPR005709; Ribosomal_S4_bac-type.
DR   InterPro; IPR018079; Ribosomal_S4_CS.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   PANTHER; PTHR11831; PTHR11831; 1.
DR   PANTHER; PTHR11831:SF4; PTHR11831:SF4; 1.
DR   Pfam; PF00163; Ribosomal_S4; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM01390; Ribosomal_S4; 1.
DR   SMART; SM00363; S4; 1.
DR   TIGRFAMs; TIGR01017; rpsD_bact; 1.
DR   PROSITE; PS00632; RIBOSOMAL_S4; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Repressor; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding;
KW   Transcription; Transcription regulation; Transcription termination;
KW   Translation regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..206
FT                   /note="30S ribosomal protein S4"
FT                   /id="PRO_0000132381"
FT   DOMAIN          96..156
FT                   /note="S4 RNA-binding"
SQ   SEQUENCE   206 AA;  23469 MW;  4015969DF8E582BB CRC64;
     MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK
     VRRIYGVLER QFRNYYKEAA RLKGNTGENL LALLEGRLDN VVYRMGFGAT RAEARQLVSH
     KAIMVNGRVV NIASYQVSPN DVVSIREKAK KQSRVKAALE LAEQREKPTW LEVDAGKMEG
     TFKRKPERSD LSADINEHLI VELYSK