BAMA_THAAR
ID BAMA_THAAR Reviewed; 377 AA.
AC O87872;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase {ECO:0000303|PubMed:10406950};
DE EC=3.7.1.21 {ECO:0000269|PubMed:10406950};
DE AltName: Full=6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase {ECO:0000305};
DE AltName: Full=Beta-oxoacyl-CoA hydrolase {ECO:0000303|PubMed:10406950};
DE Short=Oah {ECO:0000303|PubMed:10406950};
GN Name=oah;
OS Thauera aromatica.
OC Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Thauera.
OX NCBI_TaxID=59405;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-8, AND PATHWAY.
RC STRAIN=DSM 6984 / CIP 107765 / K172;
RX PubMed=9746358; DOI=10.1046/j.1432-1327.1998.2560148.x;
RA Breese K., Boll M., Alt-Moerbe J., Schaegger H., Fuchs G.;
RT "Genes coding for the benzoyl-CoA pathway of anaerobic aromatic metabolism
RT in the bacterium Thauera aromatica.";
RL Eur. J. Biochem. 256:148-154(1998).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBUNIT.
RC STRAIN=DSM 6984 / CIP 107765 / K172;
RX PubMed=10406950; DOI=10.1046/j.1432-1327.1999.00504.x;
RA Laempe D., Jahn M., Fuchs G.;
RT "6-Hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase and 6-oxocyclohex-1-
RT ene-1-carbonyl-CoA hydrolase, enzymes of the benzoyl-CoA pathway of
RT anaerobic aromatic metabolism in the denitrifying bacterium Thauera
RT aromatica.";
RL Eur. J. Biochem. 263:420-429(1999).
CC -!- FUNCTION: Involved in the central benzoyl-CoA catabolism. Catalyzes the
CC addition of one molecule of water to the double bond and the hydrolytic
CC cleavage of C-C bond in the alicyclic ring, 6-oxocyclohex-1-ene-1-
CC carbonyl-CoA (6-OCH-CoA) to yield 3-hydroxypimelyl-CoA.
CC {ECO:0000269|PubMed:10406950}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O = 3-hydroxy-6-
CC carboxyhexanoyl-CoA + H(+); Xref=Rhea:RHEA:39651, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57343, ChEBI:CHEBI:76526; EC=3.7.1.21;
CC Evidence={ECO:0000269|PubMed:10406950};
CC -!- PATHWAY: Aromatic compound metabolism; benzoyl-CoA degradation.
CC {ECO:0000305|PubMed:9746358}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:10406950}.
CC -!- SIMILARITY: Belongs to the enoyl-CoA hydratase/isomerase family.
CC {ECO:0000305}.
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DR EMBL; AJ224959; CAA12245.1; -; Genomic_DNA.
DR AlphaFoldDB; O87872; -.
DR SMR; O87872; -.
DR KEGG; ag:CAA12245; -.
DR BRENDA; 3.7.1.21; 6271.
DR UniPathway; UPA00739; -.
DR GO; GO:0018807; F:6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0016823; F:hydrolase activity, acting on acid carbon-carbon bonds, in ketonic substances; IDA:UniProtKB.
DR GO; GO:1901788; P:benzoyl-CoA catabolic process; IDA:UniProtKB.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR017613; Dearomat_hydrolase.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF52096; SSF52096; 1.
DR TIGRFAMs; TIGR03200; dearomat_oah; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Hydrolase.
FT CHAIN 1..377
FT /note="6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase"
FT /id="PRO_0000430865"
SQ SEQUENCE 377 AA; 41477 MW; B4B1818B61B05437 CRC64;
MNPTTQKLVE QNAPAQLVDH NLVPETVCPG VLYEKRPARN LKGEVVPGLY NVWISLDNPK
QYNSYTTDMV KGLILAFRAA SCARDVASVV FTAVGDKAFC TGGNTKEYAE YYAGNPQEYR
QYMRLFNDMV SAILGCDKPV ICRVNGMRIG GGQEIGMAAD FTVAQDLANF GQAGPKHGSA
AIGGATDFLP LMIGCEQAMV SGTLCEPFSA HKANRLGICM QIVPALKVDG KFIANPLVVT
DRYLDEFGRI IHGEFKTGDE LAAGKELMKR GEIDLSLLDE AVEKLCAKLI STFPECLTKS
FEELRKPKLD AWNRNKENSR AWLALNMMNE ARTGFRAFNE GNKETGREIE FTDLRQALAK
GMPWTPELIE SLMPGAK
