RS4_SALTY
ID RS4_SALTY Reviewed; 206 AA.
AC O54297;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=30S ribosomal protein S4;
GN Name=rpsD; OrderedLocusNames=STM3416;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS.
RC STRAIN=LT2;
RX PubMed=9520473; DOI=10.1073/pnas.95.7.3949;
RA Bjoerkman J., Hughes D., Andersson D.I.;
RT "Virulence of antibiotic-resistant Salmonella typhimurium.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:3949-3953(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP CHARACTERIZATION OF VARIANTS.
RC STRAIN=LT2;
RX PubMed=9987109; DOI=10.1046/j.1365-2958.1999.01142.x;
RA Bjoerkman J., Samuelsson P., Andersson D.I., Hughes D.;
RT "Novel ribosomal mutations affecting translational accuracy, antibiotic
RT resistance and virulence of Salmonella typhimurium.";
RL Mol. Microbiol. 31:53-58(1999).
RN [4]
RP REVIEW ON TRANSLATIONAL ACCURACY.
RA Kurland C.G., Hughes D., Ehrenberg M.;
RT "Limitations of translational accuracy.";
RL (In) Neidhardt F.C., Curtiss R. III, Ingraham J.L., Lin E.C.C.,
RL Low K.B. Magasanik B., Reznikoff W.S., Riley M., Schaechter M.,
RL Umbarger H.E. (eds.);
RL Escherichia coli and Salmonella: Cellular and molecular biology (2nd ed.),
RL pp.979-1004, American Society for Microbiology Press, Washington D.C.
RL (1996).
CC -!- FUNCTION: One of the primary rRNA binding proteins, it binds directly
CC to 16S rRNA where it nucleates assembly of the body of the 30S subunit.
CC {ECO:0000250}.
CC -!- FUNCTION: With S5 and S12 plays an important role in translational
CC accuracy.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit. Contacts protein S5. The
CC interaction surface between S4 and S5 is involved in control of
CC translational fidelity.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS4 family.
CC {ECO:0000305}.
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DR EMBL; AJ223236; CAA11203.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22279.1; -; Genomic_DNA.
DR RefSeq; NP_462320.1; NC_003197.2.
DR RefSeq; WP_000135226.1; NC_003197.2.
DR AlphaFoldDB; O54297; -.
DR SMR; O54297; -.
DR STRING; 99287.STM3416; -.
DR PaxDb; O54297; -.
DR EnsemblBacteria; AAL22279; AAL22279; STM3416.
DR GeneID; 1254939; -.
DR GeneID; 67370906; -.
DR KEGG; stm:STM3416; -.
DR PATRIC; fig|99287.12.peg.3613; -.
DR HOGENOM; CLU_092403_0_2_6; -.
DR OMA; NVVFRMG; -.
DR PhylomeDB; O54297; -.
DR BioCyc; SENT99287:STM3416-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0015935; C:small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0045903; P:positive regulation of translational fidelity; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.10.290.10; -; 1.
DR HAMAP; MF_01306_B; Ribosomal_S4_B; 1.
DR InterPro; IPR022801; Ribosomal_S4/S9.
DR InterPro; IPR001912; Ribosomal_S4/S9_N.
DR InterPro; IPR005709; Ribosomal_S4_bac-type.
DR InterPro; IPR018079; Ribosomal_S4_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR PANTHER; PTHR11831; PTHR11831; 1.
DR PANTHER; PTHR11831:SF4; PTHR11831:SF4; 1.
DR Pfam; PF00163; Ribosomal_S4; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM01390; Ribosomal_S4; 1.
DR SMART; SM00363; S4; 1.
DR TIGRFAMs; TIGR01017; rpsD_bact; 1.
DR PROSITE; PS00632; RIBOSOMAL_S4; 1.
DR PROSITE; PS50889; S4; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Reference proteome; Ribonucleoprotein;
KW Ribosomal protein; RNA-binding; rRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..206
FT /note="30S ribosomal protein S4"
FT /id="PRO_0000132451"
FT DOMAIN 96..156
FT /note="S4 RNA-binding"
FT VARIANT 54
FT /note="Q -> L (streptomycin resistant. Also confers
FT streptomycin independence on streptomycin-dependent S12
FT mutant P91D)"
FT VARIANT 54
FT /note="Q -> P (confers streptomycin independence on
FT streptomycin-dependent S12 mutant P90R. A RAM mutant)"
FT VARIANT 200
FT /note="I -> N (confers streptomycin independence on
FT streptomycin-dependent S12 mutant P91D. A RAM mutant)"
FT VARIANT 201
FT /note="Missing (streptomycin resistant. Also confers
FT streptomycin independence on streptomycin-dependent S12
FT mutant P91D)"
FT VARIANT 202..206
FT /note="Missing (streptomycin resistant. Also confers
FT streptomycin independence on streptomycin-dependent S12
FT mutant P90L)"
FT VARIANT 206
FT /note="K -> N (in JB221; fast-growing suppressor of
FT streptomycin resistant mutant S12 K42N. A RAM mutant)"
FT VARIANT 206
FT /note="K -> T (confers streptomycin independence on
FT streptomycin-dependent S12 mutant P90R. A RAM mutant)"
SQ SEQUENCE 206 AA; 23485 MW; 5EE4799DF8E582BB CRC64;
MARYLGPKLK LSRREGTDLF LKSGVRAIDT KCKIEQAPGQ HGARKPRLSD YGVQLREKQK
VRRIYGVLER QFRNYYKEAA RLKGNTGENL LALLEGRLDN VVYRMGFGAT RAEARQLVSH
KAIMVNGRVV NIASYQVSPN DVVSIREKAK KQSRVKAALE LAEQREKPTW LEVDAGKMEG
TYKRKPERSD LSADINEHLI VELYSK
