RS5_BACSU
ID RS5_BACSU Reviewed; 166 AA.
AC P21467;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 3.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=30S ribosomal protein S5;
DE Short=BS5;
GN Name=rpsE; Synonyms=spcA; OrderedLocusNames=BSU01330;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT alpha region.";
RL Gene 169:17-23(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT SPECTINOMYCIN RESISTANCE
RP 24-VAL--LYS-26 DEL.
RC STRAIN=1A241;
RX PubMed=2139212; DOI=10.1093/nar/18.6.1647;
RA Yoshikawa H., Doi R.H.;
RT "Sequence of the Bacillus subtilis spectinomycin resistance gene region.";
RL Nucleic Acids Res. 18:1647-1647(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-39.
RC STRAIN=168;
RX PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA Kawamura F., Yoshikawa H., Takahashi H.;
RT "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT subtilis chromosome.";
RL Microbiology 142:3039-3046(1996).
RN [5]
RP PROTEIN SEQUENCE OF 1-40.
RX PubMed=6806564; DOI=10.1007/bf00330792;
RA Higo K., Otaka E., Osawa S.;
RT "Purification and characterization of 30S ribosomal proteins from Bacillus
RT subtilis: correlation to Escherichia coli 30S proteins.";
RL Mol. Gen. Genet. 185:239-244(1982).
RN [6]
RP PROTEIN SEQUENCE OF 1-46, AND VARIANTS SPECTINOMYCIN RESISTANT BPSC 31
RP ARG-30 DEL AND BPSC 61 ARG-30 DEL.
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=6211592; DOI=10.1007/bf00330787;
RA Higo K., Itoh T., Osawa S.;
RT "Alteration of ribosomal protein S5 from two spectinomycin-resistant
RT mutants of Bacillus subtilis: deletion of one amino acid residue.";
RL Mol. Gen. Genet. 185:205-206(1982).
RN [7]
RP PROTEIN SEQUENCE OF 24-29, AND VARIANT SPECTINOMYCIN RESISTANT BSPC 111
RP ILE-26.
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=131241; DOI=10.1007/bf00277301;
RA Itoh T.;
RT "Amino acid replacement in the protein S5 from a spectinomycin resistant
RT mutant of Bacillus subtilis.";
RL Mol. Gen. Genet. 144:39-42(1976).
RN [8]
RP CHARACTERIZATION, AND VARIANTS RPSE1 VAL-28; RPSE9 ARG-104; RPSE8 CYS-112
RP AND RPSE7 HIS-112.
RC STRAIN=168;
RX PubMed=11489846; DOI=10.1128/jb.183.17.4958-4963.2001;
RA Inaoka T., Kasai K., Ochi K.;
RT "Construction of an in vivo nonsense readthrough assay system and
RT functional analysis of ribosomal proteins S12, S4, and S5 in Bacillus
RT subtilis.";
RL J. Bacteriol. 183:4958-4963(2001).
RN [9] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-166 WITH AND WITHOUT
RP VIRGINIAMYCIN M, AND SUBUNIT.
RX PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT "Structural basis for antibiotic resistance mediated by the Bacillus
RT subtilis ABCF ATPase VmlR.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC -!- FUNCTION: With S4 and S12 plays an important role in translational
CC accuracy; many suppressors of streptomycin-dependent mutants of protein
CC S12 are found in this protein, some but not all of which decrease
CC translational accuracy (ram, ribosomal ambiguity mutations).
CC -!- SUBUNIT: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts
CC proteins S4 and S8 (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:30126986}.
CC -!- DOMAIN: The N-terminal domain interacts with the head of the 30S
CC subunit; the C-terminal domain interacts with the body and contacts
CC protein S4. The interaction surface between S4 and S5 is involved in
CC control of translational fidelity.
CC -!- MISCELLANEOUS: Altered S5 proteins have been identified in a number of
CC mutants. Some mutations in S5 have been shown to increase translational
CC error frequencies.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS5 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB59115.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; L47971; AAB06816.1; -; Genomic_DNA.
DR EMBL; M31102; AAB59115.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL009126; CAB11909.1; -; Genomic_DNA.
DR EMBL; D64125; BAA20856.1; -; Genomic_DNA.
DR PIR; D69699; R3BS5S.
DR RefSeq; NP_388014.1; NC_000964.3.
DR RefSeq; WP_003328273.1; NZ_JNCM01000029.1.
DR PDB; 3J9W; EM; 3.90 A; AE=1-166.
DR PDB; 5NJT; EM; 3.80 A; E=2-166.
DR PDB; 6HA1; EM; 3.10 A; e=1-166.
DR PDB; 6HA8; EM; 3.50 A; e=1-166.
DR PDB; 6HTQ; EM; 4.50 A; e=3-166.
DR PDB; 7O5B; EM; 3.33 A; E=1-166.
DR PDB; 7QV1; EM; 3.50 A; e=1-166.
DR PDB; 7QV2; EM; 3.50 A; e=1-166.
DR PDB; 7QV3; EM; 5.14 A; e=1-166.
DR PDBsum; 3J9W; -.
DR PDBsum; 5NJT; -.
DR PDBsum; 6HA1; -.
DR PDBsum; 6HA8; -.
DR PDBsum; 6HTQ; -.
DR PDBsum; 7O5B; -.
DR PDBsum; 7QV1; -.
DR PDBsum; 7QV2; -.
DR PDBsum; 7QV3; -.
DR AlphaFoldDB; P21467; -.
DR SMR; P21467; -.
DR STRING; 224308.BSU01330; -.
DR jPOST; P21467; -.
DR PaxDb; P21467; -.
DR PRIDE; P21467; -.
DR EnsemblBacteria; CAB11909; CAB11909; BSU_01330.
DR GeneID; 23412283; -.
DR GeneID; 938012; -.
DR KEGG; bsu:BSU01330; -.
DR PATRIC; fig|224308.179.peg.136; -.
DR eggNOG; COG0098; Bacteria.
DR InParanoid; P21467; -.
DR OMA; KRGCGSW; -.
DR PhylomeDB; P21467; -.
DR BioCyc; BSUB:BSU01330-MON; -.
DR PRO; PR:P21467; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0006412; P:translation; IBA:GO_Central.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_01307_B; Ribosomal_S5_B; 1.
DR InterPro; IPR000851; Ribosomal_S5.
DR InterPro; IPR005712; Ribosomal_S5_bac-type.
DR InterPro; IPR005324; Ribosomal_S5_C.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR013810; Ribosomal_S5_N.
DR InterPro; IPR018192; Ribosomal_S5_N_CS.
DR PANTHER; PTHR13718; PTHR13718; 1.
DR Pfam; PF00333; Ribosomal_S5; 1.
DR Pfam; PF03719; Ribosomal_S5_C; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR TIGRFAMs; TIGR01021; rpsE_bact; 1.
DR PROSITE; PS00585; RIBOSOMAL_S5; 1.
DR PROSITE; PS50881; S5_DSRBD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antibiotic resistance; Direct protein sequencing;
KW Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding;
KW rRNA-binding.
FT CHAIN 1..166
FT /note="30S ribosomal protein S5"
FT /id="PRO_0000131470"
FT DOMAIN 11..74
FT /note="S5 DRBM"
FT VARIANT 24..26
FT /note="Missing (in 1A241; spectinomycin resistant)"
FT /evidence="ECO:0000269|PubMed:2139212"
FT VARIANT 26
FT /note="K -> I (in BSPC 111; spectinomycin resistant)"
FT /evidence="ECO:0000269|PubMed:131241"
FT VARIANT 28
FT /note="G -> V (in rpsE1; spectinomycin restistant, not a
FT ram mutant)"
FT /evidence="ECO:0000269|PubMed:11489846"
FT VARIANT 30
FT /note="Missing (in BPSC 31 and BPSC 61; spectinomycin
FT resistant)"
FT /evidence="ECO:0000269|PubMed:6211592"
FT VARIANT 104
FT /note="G -> R (in rpsE9; suppresses S12 mutation K55D.
FT Lethal in the absence of the S12 mutation)"
FT /evidence="ECO:0000269|PubMed:11489846"
FT VARIANT 112
FT /note="R -> C (in rpsE8; suppresses S12 mutant K55D. RspE8
FT is lethal in the absence of the S12 mutation)"
FT /evidence="ECO:0000269|PubMed:11489846"
FT VARIANT 112
FT /note="R -> H (in rpsE7; suppresses S12 mutant K55D. A ram
FT mutation)"
FT /evidence="ECO:0000269|PubMed:11489846"
SQ SEQUENCE 166 AA; 17623 MW; 5D96AA53FF892AF4 CRC64;
MRRIDPSKLE LEERLVTVNR VAKVVKGGRR FRFAALVVVG DKNGHVGFGT GKAQEVPEAI
RKAVEDAKKN LIEVPMVGTT IPHEIIGRFG AGNILLKPAS EGTGVIAGGP VRAVLELAGV
ADILSKSLGS NTPINMIRAT LQGLSELKRA EDVAKLRGKS VEELLG
