ID   BAME_POLNS              Reviewed;         228 AA.
AC   B1XT70;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Outer membrane protein assembly factor BamE {ECO:0000255|HAMAP-Rule:MF_00925};
DE   Flags: Precursor;
GN   Name=bamE {ECO:0000255|HAMAP-Rule:MF_00925}; OrderedLocusNames=Pnec_0252;
OS   Polynucleobacter necessarius subsp. necessarius (strain STIR1).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Polynucleobacter.
OX   NCBI_TaxID=452638;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=STIR1;
RX   PubMed=24167248; DOI=10.1073/pnas.1316687110;
RA   Boscaro V., Felletti M., Vannini C., Ackerman M.S., Chain P.S.,
RA   Malfatti S., Vergez L.M., Shin M., Doak T.G., Lynch M., Petroni G.;
RT   "Polynucleobacter necessarius, a model for genome reduction in both free-
RT   living and symbiotic bacteria.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:18590-18595(2013).
CC   -!- FUNCTION: Part of the outer membrane protein assembly complex, which is
CC       involved in assembly and insertion of beta-barrel proteins into the
CC       outer membrane. {ECO:0000255|HAMAP-Rule:MF_00925}.
CC   -!- SUBUNIT: Part of the Bam complex. {ECO:0000255|HAMAP-Rule:MF_00925}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00925}; Lipid-anchor {ECO:0000255|HAMAP-Rule:MF_00925}.
CC   -!- SIMILARITY: Belongs to the BamE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00925}.
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DR   EMBL; CP001010; ACB43547.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1XT70; -.
DR   SMR; B1XT70; -.
DR   STRING; 452638.Pnec_0252; -.
DR   EnsemblBacteria; ACB43547; ACB43547; Pnec_0252.
DR   KEGG; pne:Pnec_0252; -.
DR   eggNOG; COG2913; Bacteria.
DR   HOGENOM; CLU_1213920_0_0_4; -.
DR   OMA; SFWEVIT; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043165; P:Gram-negative-bacterium-type cell outer membrane assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0051205; P:protein insertion into membrane; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1450.10; -; 1.
DR   HAMAP; MF_00925; OM_assembly_BamE; 1.
DR   InterPro; IPR026592; BamE.
DR   InterPro; IPR037873; BamE-like.
DR   InterPro; IPR007450; Lipoprotein_SmpA/OmlA.
DR   PANTHER; PTHR37482; PTHR37482; 1.
DR   Pfam; PF04355; SmpA_OmlA; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00925"
FT   CHAIN           30..228
FT                   /note="Outer membrane protein assembly factor BamE"
FT                   /id="PRO_5000330995"
FT   REGION          197..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           30
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00925"
FT   LIPID           30
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00925"
SQ   SEQUENCE   228 AA;  25145 MW;  A2A59B8ECA0BB209 CRC64;
     MNPILKGVYS PARLGVVALT LFGILGVTGC STAVDETQRA WMNKIFRPYV PDVVQGNFIS
     SEQYAKLQLG MTREQVRQIM GTPLLASYFH ANRWDYVFEF KRSGQTVGKE RHVTVFFDGD
     KVVKFEGDAL PTEVELVAEI DNYSKSKRSF WEVITDTNKL PVTPPLQQPE VLVTSKTDNL
     PAGAAVPAAN TSGSFWDFFG SSKKDPDPQS PQLGPGTLND VPKPADSK