ID   BAME_PSEFL              Reviewed;         175 AA.
AC   O68564;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Outer membrane protein assembly factor BamE {ECO:0000255|HAMAP-Rule:MF_00925};
DE   Flags: Precursor;
GN   Name=bamE {ECO:0000255|HAMAP-Rule:MF_00925}; Synonyms=omlA, oprX;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 15453 / CIP 104605 / 52-1C;
RX   PubMed=9973334; DOI=10.1128/jb.181.4.1099-1109.1999;
RA   Ochsner U.A., Vasil A.I., Johnson Z., Vasil M.L.;
RT   "Pseudomonas aeruginosa fur overlaps with a gene encoding a novel outer
RT   membrane lipoprotein, OmlA.";
RL   J. Bacteriol. 181:1099-1109(1999).
CC   -!- FUNCTION: Part of the outer membrane protein assembly complex, which is
CC       involved in assembly and insertion of beta-barrel proteins into the
CC       outer membrane (By similarity). May have a structural role in
CC       maintaining the cell envelope integrity. {ECO:0000255|HAMAP-
CC       Rule:MF_00925, ECO:0000269|PubMed:9973334}.
CC   -!- SUBUNIT: Part of the Bam complex. {ECO:0000255|HAMAP-Rule:MF_00925}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|HAMAP-
CC       Rule:MF_00925}; Lipid-anchor {ECO:0000255|HAMAP-Rule:MF_00925}.
CC   -!- SIMILARITY: Belongs to the BamE family. {ECO:0000255|HAMAP-
CC       Rule:MF_00925}.
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DR   EMBL; AF050677; AAC05681.1; -; Genomic_DNA.
DR   AlphaFoldDB; O68564; -.
DR   SMR; O68564; -.
DR   STRING; 690597.JH730984_gene333; -.
DR   eggNOG; COG2913; Bacteria.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043165; P:Gram-negative-bacterium-type cell outer membrane assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0051205; P:protein insertion into membrane; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1450.10; -; 1.
DR   HAMAP; MF_00925; OM_assembly_BamE; 1.
DR   InterPro; IPR026592; BamE.
DR   InterPro; IPR037873; BamE-like.
DR   InterPro; IPR007450; Lipoprotein_SmpA/OmlA.
DR   PANTHER; PTHR37482; PTHR37482; 1.
DR   Pfam; PF04355; SmpA_OmlA; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00925"
FT   CHAIN           22..175
FT                   /note="Outer membrane protein assembly factor BamE"
FT                   /id="PRO_0000032816"
FT   REGION          117..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          156..175
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           22
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00925"
FT   LIPID           22
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00925"
SQ   SEQUENCE   175 AA;  19123 MW;  B03950C6931F57E7 CRC64;
     MQNTKLLLTS FTFVGLLALA GCSFPGVYKI DIQQGNVVTQ DMIDQLRPGM TRRQVRFIMG
     NALLTDTFHA DRWDYLYSLQ PGGGERQQER VSVIFNGNDQ LVSLSGDFMP GVSRDEALLG
     KDSGTNVTAP AQEAEKPKSE VPAKPGSLLD TIQKDIDNVE TVPVPTPEPL DTSPQ