ID   BAMI_PSEAE              Reviewed;         219 AA.
AC   Q9I061;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Biofilm-associated metzincin protease inhibitor {ECO:0000303|PubMed:25488299};
GN   Name=bamI {ECO:0000303|PubMed:25488299};
GN   OrderedLocusNames=PA2782 {ECO:0000312|EMBL:AAG06170.1};
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND INTERACTION WITH MEP72.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=25488299; DOI=10.1128/jb.02404-14;
RA   Passmore I.J., Nishikawa K., Lilley K.S., Bowden S.D., Chung J.C.,
RA   Welch M.;
RT   "Mep72, a metzincin protease that is preferentially secreted by biofilms of
RT   Pseudomonas aeruginosa.";
RL   J. Bacteriol. 197:762-773(2015).
CC   -!- FUNCTION: Inhibitor of the metalloendopeptidase Mep72. Forms a protein-
CC       protein complex with the protease, which is the product of its
CC       coregulated adjacent gene, and probably prevents premature protease
CC       activity until the protein has been secreted.
CC       {ECO:0000269|PubMed:25488299}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Constitutes an operon together with PA2783. Its expression
CC       is up-regulated in biofilms, while it is poorly expressed in planktonic
CC       cells. {ECO:0000269|PubMed:25488299}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE004091; AAG06170.1; -; Genomic_DNA.
DR   PIR; E83299; E83299.
DR   RefSeq; NP_251472.1; NC_002516.2.
DR   RefSeq; WP_003124476.1; NZ_QZGE01000011.1.
DR   AlphaFoldDB; Q9I061; -.
DR   SMR; Q9I061; -.
DR   PaxDb; Q9I061; -.
DR   DNASU; 882771; -.
DR   EnsemblBacteria; AAG06170; AAG06170; PA2782.
DR   GeneID; 882771; -.
DR   KEGG; pae:PA2782; -.
DR   PATRIC; fig|208964.12.peg.2921; -.
DR   PseudoCAP; PA2782; -.
DR   HOGENOM; CLU_1513773_0_0_6; -.
DR   OMA; LETHITL; -.
DR   BioCyc; PAER208964:G1FZ6-2830-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005615; C:extracellular space; IDA:PseudoCAP.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008191; F:metalloendopeptidase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Metalloenzyme inhibitor;
KW   Metalloprotease inhibitor; Protease inhibitor; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..219
FT                   /note="Biofilm-associated metzincin protease inhibitor"
FT                   /id="PRO_0000452833"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          191..219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   219 AA;  22728 MW;  D0022BAC802062A5 CRC64;
     MAKTWIYAAS AAAIGGALIG GWLLDPAPPE ASPQARQSPA AQAAAAPTAA LAAPAADATR
     MLAPTPVTTP APRERVTLWQ GELRSREGAQ GIPEYLAQVE PALLDTLALG QVLEMSLPGR
     ERPLQARLAS THNSAGLPVW RGGLVDGDEA ESLTVVRGSL ETHINVATLD GSYSIIVDNR
     SGKTRVIDEN DIAARSDPHG DHVDAPLAEL PPMPPPAQG