ID   BAMS_GLYGL              Reviewed;         765 AA.
AC   Q9MB42;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Beta-amyrin synthase;
DE            EC=5.4.99.39;
GN   Name=GgbAS1;
OS   Glycyrrhiza glabra (Licorice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Galegeae; Glycyrrhiza.
OX   NCBI_TaxID=49827;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=11510484; DOI=10.1248/bpb.24.912;
RA   Hayashi H., Huang P., Kirakosyan A., Inoue K., Hiraoka N., Ikeshiro Y.,
RA   Kushiro T., Shibuya M., Ebizuka Y.;
RT   "Cloning and characterization of a cDNA encoding beta-amyrin synthase
RT   involved in glycyrrhizin and soyasaponin biosyntheses in licorice.";
RL   Biol. Pharm. Bull. 24:912-916(2001).
RN   [2]
RP   TISSUE SPECIFICITY, INDUCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=15256745; DOI=10.1248/bpb.27.1086;
RA   Hayashi H., Huang P., Takada S., Obinata M., Inoue K., Shibuya M.,
RA   Ebizuka Y.;
RT   "Differential expression of three oxidosqualene cyclase mRNAs in
RT   Glycyrrhiza glabra.";
RL   Biol. Pharm. Bull. 27:1086-1092(2004).
CC   -!- FUNCTION: Oxidosqualene cyclase converting oxidosqualene into beta-
CC       amyrin, generating five rings and eight asymmetric centers in a single
CC       transformation. Required for the production of soyasaponins and
CC       glycyrrhizin. {ECO:0000269|PubMed:11510484}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = beta-amyrin; Xref=Rhea:RHEA:31007,
CC         ChEBI:CHEBI:10352, ChEBI:CHEBI:15441; EC=5.4.99.39;
CC         Evidence={ECO:0000269|PubMed:11510484};
CC   -!- TISSUE SPECIFICITY: Highly expressed in thickened roots and root
CC       nodules. Detected in roots, hypocotyls and cotyledons of young
CC       seedlings. {ECO:0000269|PubMed:15256745}.
CC   -!- DEVELOPMENTAL STAGE: Peak of expression 2 days after germination. High
CC       levels of expression when aerial parts are growing.
CC       {ECO:0000269|PubMed:15256745}.
CC   -!- INDUCTION: Up-regulated by methyl jasmonate (MeJA) and down-regulated
CC       by gibberellin A3 (GA3). {ECO:0000269|PubMed:15256745}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB037203; BAA89815.1; -; mRNA.
DR   AlphaFoldDB; Q9MB42; -.
DR   SMR; Q9MB42; -.
DR   KEGG; ag:BAA89815; -.
DR   BRENDA; 5.4.99.39; 2487.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0031559; F:oxidosqualene cyclase activity; IEA:UniProt.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..765
FT                   /note="Beta-amyrin synthase"
FT                   /id="PRO_0000413968"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          514..556
FT                   /note="PFTB 2"
FT   REPEAT          591..631
FT                   /note="PFTB 3"
FT   REPEAT          640..681
FT                   /note="PFTB 4"
FT   REPEAT          702..743
FT                   /note="PFTB 5"
FT   ACT_SITE        485
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   765 AA;  87516 MW;  2DA78FEF3DA24F8B CRC64;
     MWRLKIAEGG KDPYIYSTNN FVGRQTWEYD PDGGTPEERA QVDAARLHFY NNRFQVKPCG
     DLLWRFQILR ENNFKQTIAS VKIGDGEEIT YEKATTAVRR AAHHLSALQT SDGHWPAQIA
     GPLFFLPPLV FCMYITGHLD SVFPEEYRKE ILRYIYYHQN EDGGWGLHIE GHSTMFCTAL
     NYICMRILGE GPDGGQDNAC ARARKWIHDH GGVTHIPSWG KTWLSILGVF DWCGSNPMPP
     EFWILPSFLP MHPAKMWCYC RLVYMPMSYL YGKRFVGPIT PLILQLREEL FTEPYEKVNW
     KKARHQCAKE DLYYPHPLLQ DLIWDSLYLF TEPLLTRWPF NKLVREKALQ VTMKHIHYED
     ETSRYITIGC VEKVLCMLAC WVEDPNGDAF KKHLARVPDY LWVSEDGMTM QSFGSQEWDA
     GFAVQALLAT NLVEEIAPTL AKGHDFIKKS QVRDNPSGDF KSMYRHISKG SWTFSDQDHG
     WQVSDCTAEG LKCCLLLSML PPEIVGEKME PERLYDSVNV LLSLQSKKGG LSAWEPAGAQ
     EWLELLNPTE FFADIVVEHE YVECTGSAIQ ALVLFKKLYP GHRKKEIENF IANAVRFLED
     TQTADGSWYG NWGVCFTYGS WFALGGLAAA GKTFANCAAI RKAVKFLLTT QREDGGWGES
     YLSSPKKIYV PLEGSRSNVV HTAWALMGLI HAGQAERDPA PLHRAAKLII NSQLEEGDWP
     QQEITGVFMK NCMLHYPMYR DIYPMWALAE YRRRVPLPST PVCLT