ID   BAMS_PEA                Reviewed;         758 AA.
AC   Q9LRH8;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Beta-amyrin synthase;
DE            EC=5.4.99.39;
GN   Name=OSCPSY;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10848960; DOI=10.1046/j.1432-1327.2000.01357.x;
RA   Morita M., Shibuya M., Kushiro T., Masuda K., Ebizuka Y.;
RT   "Molecular cloning and functional expression of triterpene synthases from
RT   pea (Pisum sativum) new alpha-amyrin-producing enzyme is a multifunctional
RT   triterpene synthase.";
RL   Eur. J. Biochem. 267:3453-3460(2000).
CC   -!- FUNCTION: Oxidosqualene cyclase converting oxidosqualene into beta-
CC       amyrin, generating five rings and eight asymmetric centers in a single
CC       transformation. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = beta-amyrin; Xref=Rhea:RHEA:31007,
CC         ChEBI:CHEBI:10352, ChEBI:CHEBI:15441; EC=5.4.99.39;
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB034802; BAA97558.1; -; mRNA.
DR   AlphaFoldDB; Q9LRH8; -.
DR   SMR; Q9LRH8; -.
DR   PRIDE; Q9LRH8; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0031559; F:oxidosqualene cyclase activity; IEA:UniProt.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   2: Evidence at transcript level;
KW   Isomerase; Repeat.
FT   CHAIN           1..758
FT                   /note="Beta-amyrin synthase"
FT                   /id="PRO_0000413969"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          514..556
FT                   /note="PFTB 2"
FT   REPEAT          591..631
FT                   /note="PFTB 3"
FT   REPEAT          640..681
FT                   /note="PFTB 4"
FT   ACT_SITE        485
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   758 AA;  87244 MW;  D8F10B1859DA2EB3 CRC64;
     MWRLKIAEGG NDPYLFSTNN FVGRQTWEYD PEAGSEEERA QVEEARRNFY NNRFEVKPCG
     DLLWRFQVLR ENNFKQTIGG VKIEDEEEIT YEKTTTTLRR GTHHLATLQT SDGHWPAQIA
     GPLFFMPPLV FCVYITGHLD SVFPPEHRKE ILRYIYCHQN EDGGWGLHIE GHSTMFCTAL
     NYICMRILGE GPDGGEDNAC VRARNWIRQH GGVTHIPSWG KTWLSILGVF DWLGSNPMPP
     EFWILPSFLP MHPAKMWCYC RLVYMPMSYL YGKRFVGPIT PLILQLREEL HTEPYEKINW
     TKTRHLCAKE DIYYPHPLIQ DLIWDSLYIF TEPLLTRWPF NKLVRKRALE VTMKHIHYED
     ENSRYLTIGC VEKVLCMLAC WVEDPNGDAF KKHIARVPDY LWISEDGMTM QSFGSQEWDA
     GFAVQALLAT NLIEEIKPAL AKGHDFIKKS QVTENPSGDF KSMHRHISKG SWTFSDQDHG
     WQVSDCTAEG LKCCLLLSLL PPEIVGEKME PERLFDSVNL LLSLQSKKGG LAAWEPAGAQ
     EWLELLNPTE FFADIVVEHE YVECTGSAIQ ALVLFKKLYP GHRKKEIENF IFNAVRFLED
     TQTEDGSWYG NWGVCFTYGS WFALGGLAAA GKTYTNCAAI RKGVKFLLTT QREDGGWGES
     YLSSPKKIYV PLEGNRSNVV HTAWALMGLI HAGQSERDPT PLHRAAKLLI NSQLEQGDWP
     QQEITGVFMK NCMLHYPMYR DIYPLWALAE YRRRVPLP