BAM_DROME
ID BAM_DROME Reviewed; 442 AA.
AC P22745; Q2PNQ8; Q2PNQ9; Q2PNR5; Q2PNR8; Q2PNR9; Q2PNS3; Q2PNS6; Q2PNS8;
AC Q2PNT1; Q8MRX8; Q9VBV0;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein bag-of-marbles;
GN Name=bam; ORFNames=CG10422;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, VARIANT
RP SER-239, AND DISRUPTION PHENOTYPE.
RC STRAIN=fs(3)Neo61; TISSUE=Ovary;
RX PubMed=2279698; DOI=10.1101/gad.4.12b.2242;
RA McKearin D.M., Spradling A.C.;
RT "Bag-of-marbles: a Drosophila gene required to initiate both male and
RT female gametogenesis.";
RL Genes Dev. 4:2242-2251(1990).
RN [2]
RP SEQUENCE REVISION TO 99-113.
RA McKearin D.M.;
RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-45; ASP-54; SER-239;
RP TYR-243 AND ARG-394.
RC STRAIN=Wpg1, Wpg10, Wpg11, Wpg12, Wpg13, Wpg14, Wpg15, Wpg16, Wpg2, Wpg3,
RC Wpg4, Wpg5, Wpg6, Wpg7, Wpg8, Wpg9, Zim10, Zim18, Zim22, Zim32, Zim35,
RC Zim49, Zim5, and Zim7;
RX PubMed=16357040; DOI=10.1093/molbev/msj074;
RA Civetta A., Rajakumar S.A., Brouwers B., Bacik J.P.;
RT "Rapid evolution and gene-specific patterns of selection for three genes of
RT spermatogenesis in Drosophila.";
RL Mol. Biol. Evol. 23:655-662(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Required to initiate both male and female gametogenesis. May
CC regulate cystoblast cell divisions. {ECO:0000269|PubMed:2279698}.
CC -!- INTERACTION:
CC P22745; Q9W1I2: bgcn; NbExp=13; IntAct=EBI-88504, EBI-142483;
CC P22745; Q9V345: CSN4; NbExp=5; IntAct=EBI-88504, EBI-141466;
CC P22745; Q02748: eIF4A; NbExp=4; IntAct=EBI-88504, EBI-85570;
CC -!- TISSUE SPECIFICITY: In cystoblasts and/or very early cystocytes in
CC ovary and testis. {ECO:0000269|PubMed:2279698}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit abnormal cysts, they contain an
CC excess number of cells that cannot differentiate into gametes.
CC {ECO:0000269|PubMed:2279698}.
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DR EMBL; X56202; CAA39662.1; -; Genomic_DNA.
DR EMBL; DQ315978; ABC48725.1; -; Genomic_DNA.
DR EMBL; DQ315979; ABC48726.1; -; Genomic_DNA.
DR EMBL; DQ315980; ABC48727.1; -; Genomic_DNA.
DR EMBL; DQ315981; ABC48728.1; -; Genomic_DNA.
DR EMBL; DQ315982; ABC48729.1; -; Genomic_DNA.
DR EMBL; DQ315983; ABC48730.1; -; Genomic_DNA.
DR EMBL; DQ315984; ABC48731.1; -; Genomic_DNA.
DR EMBL; DQ315985; ABC48732.1; -; Genomic_DNA.
DR EMBL; DQ315986; ABC48733.1; -; Genomic_DNA.
DR EMBL; DQ315987; ABC48734.1; -; Genomic_DNA.
DR EMBL; DQ315988; ABC48735.1; -; Genomic_DNA.
DR EMBL; DQ315989; ABC48736.1; -; Genomic_DNA.
DR EMBL; DQ315990; ABC48737.1; -; Genomic_DNA.
DR EMBL; DQ315991; ABC48738.1; -; Genomic_DNA.
DR EMBL; DQ315992; ABC48739.1; -; Genomic_DNA.
DR EMBL; DQ315993; ABC48740.1; -; Genomic_DNA.
DR EMBL; DQ315994; ABC48741.1; -; Genomic_DNA.
DR EMBL; DQ315995; ABC48742.1; -; Genomic_DNA.
DR EMBL; DQ315996; ABC48743.1; -; Genomic_DNA.
DR EMBL; DQ315997; ABC48744.1; -; Genomic_DNA.
DR EMBL; DQ315998; ABC48745.1; -; Genomic_DNA.
DR EMBL; DQ315999; ABC48746.1; -; Genomic_DNA.
DR EMBL; DQ316000; ABC48747.1; -; Genomic_DNA.
DR EMBL; DQ316001; ABC48748.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF56427.1; -; Genomic_DNA.
DR EMBL; AY119208; AAM51068.1; -; mRNA.
DR PIR; A36469; A36469.
DR RefSeq; NP_476800.1; NM_057452.3.
DR PDB; 5ONA; X-ray; 2.70 A; C/F=13-36.
DR PDB; 5ONB; X-ray; 3.00 A; B/D/F/H=13-36.
DR PDBsum; 5ONA; -.
DR PDBsum; 5ONB; -.
DR AlphaFoldDB; P22745; -.
DR SMR; P22745; -.
DR BioGRID; 67957; 45.
DR DIP; DIP-48889N; -.
DR IntAct; P22745; 3.
DR STRING; 7227.FBpp0084243; -.
DR PaxDb; P22745; -.
DR PRIDE; P22745; -.
DR DNASU; 43038; -.
DR EnsemblMetazoa; FBtr0084869; FBpp0084243; FBgn0000158.
DR GeneID; 43038; -.
DR KEGG; dme:Dmel_CG10422; -.
DR CTD; 43038; -.
DR FlyBase; FBgn0000158; bam.
DR VEuPathDB; VectorBase:FBgn0000158; -.
DR eggNOG; ENOG502TB7D; Eukaryota.
DR HOGENOM; CLU_644469_0_0_1; -.
DR InParanoid; P22745; -.
DR OMA; FNWEMDL; -.
DR OrthoDB; 987870at2759; -.
DR PhylomeDB; P22745; -.
DR SignaLink; P22745; -.
DR BioGRID-ORCS; 43038; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43038; -.
DR PRO; PR:P22745; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0000158; Expressed in cleaving embryo and 10 other tissues.
DR ExpressionAtlas; P22745; baseline and differential.
DR Genevisible; P22745; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0045169; C:fusome; IDA:FlyBase.
DR GO; GO:0045170; C:spectrosome; IDA:FlyBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:FlyBase.
DR GO; GO:0000900; F:mRNA regulatory element binding translation repressor activity; IDA:FlyBase.
DR GO; GO:0043130; F:ubiquitin binding; IDA:FlyBase.
DR GO; GO:0035212; P:cell competition in a multicellular organism; IMP:FlyBase.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0007282; P:cystoblast division; IMP:FlyBase.
DR GO; GO:0048132; P:female germ-line stem cell asymmetric division; IMP:FlyBase.
DR GO; GO:0045478; P:fusome organization; IMP:FlyBase.
DR GO; GO:0007276; P:gamete generation; IMP:FlyBase.
DR GO; GO:0042078; P:germ-line stem cell division; IMP:FlyBase.
DR GO; GO:0030718; P:germ-line stem cell population maintenance; IDA:FlyBase.
DR GO; GO:0030727; P:germarium-derived female germ-line cyst formation; IMP:FlyBase.
DR GO; GO:0098730; P:male germline stem cell symmetric division; IMP:FlyBase.
DR GO; GO:0061060; P:negative regulation of peptidoglycan recognition protein signaling pathway; IMP:FlyBase.
DR GO; GO:0017148; P:negative regulation of translation; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:1903003; P:positive regulation of protein deubiquitination; IMP:FlyBase.
DR GO; GO:1903006; P:positive regulation of protein K63-linked deubiquitination; IMP:FlyBase.
DR GO; GO:0045880; P:positive regulation of smoothened signaling pathway; IGI:FlyBase.
DR GO; GO:2000738; P:positive regulation of stem cell differentiation; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR GO; GO:0007284; P:spermatogonial cell division; IMP:FlyBase.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Developmental protein; Differentiation;
KW Oogenesis; Reference proteome; Spermatogenesis.
FT CHAIN 1..442
FT /note="Protein bag-of-marbles"
FT /id="PRO_0000064821"
FT REGION 408..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 45
FT /note="C -> S (in strain: Wpg6, Wpg8 and Wpg12)"
FT /evidence="ECO:0000269|PubMed:16357040"
FT VARIANT 54
FT /note="G -> D (in strain: Zim10)"
FT /evidence="ECO:0000269|PubMed:16357040"
FT VARIANT 239
FT /note="A -> S (in strain: fs(3)Neo61, Wpg1, Wpg10, Wpg16,
FT Wpg2, Wpg3, Zim10, Zim18, Zim32 and Zim35)"
FT /evidence="ECO:0000269|PubMed:16357040,
FT ECO:0000269|PubMed:2279698"
FT VARIANT 243
FT /note="F -> Y (in strain: Wpg4)"
FT /evidence="ECO:0000269|PubMed:16357040"
FT VARIANT 394
FT /note="P -> R (in strain: Zim10)"
FT /evidence="ECO:0000269|PubMed:16357040"
FT CONFLICT 439
FT /note="R -> A (in Ref. 1; CAA39662)"
FT /evidence="ECO:0000305"
FT HELIX 14..33
FT /evidence="ECO:0007829|PDB:5ONA"
SQ SEQUENCE 442 AA; 50275 MW; D2FE2BCFC249F254 CRC64;
MLNARDVCPE GNDDQQLDHN FKQMEEHLAL MVEGNENEDP RKATCEYEDT NEDGATCTSG
VLSEIQENFG RLRLCDVTAP LLEFHGLDCL QQIQKRSRHF AFDGSPAKKS RSGGVLVTGP
KQKQLQKENV WNRKSKGSAS ADNIEKLPIT IEKLHMIGLH GDCLEHNAVL RLMNLFRSLH
DHLTADLGFS RQNSMPSDYL FDMPVKSTMP KSLNVRYQLQ VLCTKVERFL VQQRRTLEAN
RHFDFEKYDE CDKLLKGFAS YLDNFKLLLK PKMRNRNGNS GSNADKFHTQ RMERLLIGLR
DWIKAAHLSV HVFNWEMDLE HRYSGAMTES HKSLNERAIL LSGAELRAAE ARGISAEDLF
IAQRYKLGGP IYCVLEQHEF LSALIANPET YFPPSVVAIC GPQKLGAVSM EQPSASEEEF
EETEEVPSSP PRHTGRVPRF RS
