ID   BARF1_EBVG              Reviewed;         221 AA.
AC   P0CW72; Q777A5;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   03-MAY-2011, sequence version 1.
DT   02-JUN-2021, entry version 41.
DE   RecName: Full=Secreted protein BARF1;
DE   AltName: Full=33 kDa early protein;
DE   AltName: Full=p33;
DE   Flags: Precursor;
GN   Name=BARF1;
OS   Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus.
OX   NCBI_TaxID=10376;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16306603; DOI=10.1128/jvi.79.24.15323-15330.2005;
RA   Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H.,
RA   Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.;
RT   "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a
RT   nasopharyngeal carcinoma patient.";
RL   J. Virol. 79:15323-15330(2005).
CC   -!- FUNCTION: Plays diverse functions in immunomodulation and oncogenicity,
CC       maybe by acting as a functional receptor for human CSF1. May inhibit
CC       interferon secretion from mononuclear cells. Exhibits oncogenic
CC       activity in vitro (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Homohexamer. Interacts with human CSF1 (By similarity).
CC       {ECO:0000250}.
CC   -!- INTERACTION:
CC       P0CW72; P0CW72: BARF1; NbExp=2; IntAct=EBI-2620133, EBI-2620133;
CC       P0CW72; P09603: CSF1; Xeno; NbExp=3; IntAct=EBI-2620133, EBI-2872294;
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=Massively secreted in the serum of
CC       EBV-induced nasophyryngeal carcinoma patients. {ECO:0000250}.
CC   -!- PTM: Phosphorylated on serine and threonine by host. {ECO:0000250}.
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DR   EMBL; AY961628; AAY41158.1; -; Genomic_DNA.
DR   RefSeq; YP_401719.1; NC_007605.1.
DR   PDB; 4FA8; X-ray; 2.20 A; A/B/D=19-221.
DR   PDBsum; 4FA8; -.
DR   SMR; P0CW72; -.
DR   DIP; DIP-60066N; -.
DR   IntAct; P0CW72; 5.
DR   DNASU; 3783772; -.
DR   GeneID; 3783772; -.
DR   KEGG; vg:3783772; -.
DR   Proteomes; UP000007641; Genome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   Gene3D; 2.60.40.10; -; 2.
DR   InterPro; IPR007110; Ig-like_dom.
DR   InterPro; IPR036179; Ig-like_dom_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   SUPFAM; SSF48726; SSF48726; 2.
DR   PROSITE; PS50835; IG_LIKE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Early protein; Glycoprotein;
KW   Immunoglobulin domain; Oncogene; Repeat; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..221
FT                   /note="Secreted protein BARF1"
FT                   /id="PRO_0000408390"
FT   DOMAIN          21..120
FT                   /note="Ig-like 1"
FT   DOMAIN          124..220
FT                   /note="Ig-like 2"
FT   CARBOHYD        95
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT   DISULFID        146..201
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT   STRAND          22..24
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          29..34
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          44..52
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   TURN            53..55
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          56..64
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          67..70
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   HELIX           72..74
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          78..83
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          86..93
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   HELIX           96..98
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          100..108
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          111..133
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          142..152
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          155..158
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          183..191
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          199..207
FT                   /evidence="ECO:0007829|PDB:4FA8"
FT   STRAND          209..216
FT                   /evidence="ECO:0007829|PDB:4FA8"
SQ   SEQUENCE   221 AA;  24471 MW;  CA5A24D1EA28758E CRC64;
     MARFIAQLLL LASCVAAGQA VTAFLGERVT LTSYWRRVSL GPEIEVSWFK LGPGEEQVLI
     GRMHHDVIFI EWPFRGFFDI HRSANTFFLV VTAANISHDG NYLCRMKLGE TEVTKQEHLS
     VVKPLTLSVH SERSQFPDFS VLTVTCTVNA FPHPHVQWLM PEGVEPAPTA ANGGVMKEKD
     GSLSVAVDLS LPKPWHLPVT CVGKNDKEEA HGVYVSGYLS Q