BARH1_DROME
ID BARH1_DROME Reviewed; 544 AA.
AC Q24255; Q9VX54;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Homeobox protein B-H1;
DE AltName: Full=Homeobox protein BarH1;
GN Name=B-H1; Synonyms=barh1; ORFNames=CG5529;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAL13538.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Canton-S {ECO:0000269|PubMed:1674606};
RX PubMed=1674606; DOI=10.1073/pnas.88.10.4343;
RA Saigo K., Emori Y., Sone M., Akimaru H., Takayama E., Higashijima S.,
RA Ishimaru S., Kojima T.;
RT "Identification of a different-type homeobox gene, BarH1, possibly causing
RT Bar (B) and Om(1D) mutations in Drosophila.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:4343-4347(1991).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S {ECO:0000269|PubMed:1346120};
RX PubMed=1346120; DOI=10.1101/gad.6.1.50;
RA Higashijima S., Kojima T., Michiue T., Ishimaru S., Emori Y., Saigo K.;
RT "Dual Bar homeo box genes of Drosophila required in two photoreceptor
RT cells, R1 and R6, and primary pigment cells for normal eye development.";
RL Genes Dev. 6:50-60(1992).
RN [6] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=1350558; DOI=10.1101/gad.6.6.1005;
RA Higashijima S., Michiue T., Emori Y., Saigo K.;
RT "Subtype determination of Drosophila embryonic external sensory organs by
RT redundant homeo box genes BarH1 and BarH2.";
RL Genes Dev. 6:1005-1018(1992).
RN [7] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10068639; DOI=10.1242/dev.126.7.1457;
RA Sato M., Kojima T., Michiue T., Saigo K.;
RT "Bar homeobox genes are latitudinal prepattern genes in the developing
RT Drosophila notum whose expression is regulated by the concerted functions
RT of decapentaplegic and wingless.";
RL Development 126:1457-1466(1999).
RN [8] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=10648235; DOI=10.1242/dev.127.4.769;
RA Kojima T., Sato M., Saigo K.;
RT "Formation and specification of distal leg segments in Drosophila by dual
RT Bar homeobox genes, BarH1 and BarH2.";
RL Development 127:769-778(2000).
CC -!- FUNCTION: B-H1 and B-H2 are regulated by members of the wg signaling
CC pathway; wg and dpp. B-H1 and B-H2 are coexpressed and functionally
CC required in R1 and R6 receptor cells and primary pigment cells for
CC normal eye development. Coexpression is also required for the fate
CC determination of external sensory organs, formation of notal
CC microchaetae, formation of presutural macrochaetae, antennal
CC development and for distal leg morphogenesis; segmentation and
CC specification of tarsal segments 3-5. {ECO:0000269|PubMed:10068639,
CC ECO:0000269|PubMed:10648235, ECO:0000269|PubMed:1346120,
CC ECO:0000269|PubMed:1350558}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: B-H1 and B-H2 are abundant in the eye-antenna
CC imaginal disk. Expressed in R1 and R6 cells throughout larval stage
CC until 30 hours after puparium formation, at which time expression is
CC seen in the anterior and posterior primary pigment cells. Coexpressed
CC in embryonic glial cells, neurons of the CNS and PNS, most latitudinal
CC anterior cells of the developing notum and the central circular region
CC of the leg and antennal imaginal disk throughout larval development.
CC {ECO:0000269|PubMed:10068639, ECO:0000269|PubMed:10648235,
CC ECO:0000269|PubMed:1346120, ECO:0000269|PubMed:1350558,
CC ECO:0000269|PubMed:1674606}.
CC -!- DEVELOPMENTAL STAGE: Coexpressed at high levels with B-H1 in embryo and
CC pupae and at low levels in larvae. {ECO:0000269|PubMed:1346120,
CC ECO:0000269|PubMed:1674606}.
CC -!- SIMILARITY: Belongs to the Antp homeobox family. {ECO:0000305}.
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DR EMBL; M73079; AAA28382.1; -; Genomic_DNA.
DR EMBL; M73259; AAA28382.1; JOINED; Genomic_DNA.
DR EMBL; M73078; AAA28382.1; JOINED; Genomic_DNA.
DR EMBL; AE014298; AAF48726.1; -; Genomic_DNA.
DR EMBL; AY058309; AAL13538.1; -; mRNA.
DR PIR; B39369; B39369.
DR RefSeq; NP_523387.1; NM_078663.3.
DR AlphaFoldDB; Q24255; -.
DR SMR; Q24255; -.
DR BioGRID; 59052; 22.
DR DIP; DIP-19778N; -.
DR IntAct; Q24255; 8.
DR STRING; 7227.FBpp0074204; -.
DR PaxDb; Q24255; -.
DR DNASU; 32724; -.
DR EnsemblMetazoa; FBtr0074430; FBpp0074204; FBgn0011758.
DR GeneID; 32724; -.
DR KEGG; dme:Dmel_CG5529; -.
DR CTD; 32724; -.
DR FlyBase; FBgn0011758; B-H1.
DR VEuPathDB; VectorBase:FBgn0011758; -.
DR eggNOG; KOG0488; Eukaryota.
DR GeneTree; ENSGT00940000169370; -.
DR HOGENOM; CLU_500856_0_0_1; -.
DR InParanoid; Q24255; -.
DR OMA; NIDIYYR; -.
DR PhylomeDB; Q24255; -.
DR BioGRID-ORCS; 32724; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 32724; -.
DR PRO; PR:Q24255; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0011758; Expressed in interneuron and 38 other tissues.
DR ExpressionAtlas; Q24255; baseline and differential.
DR Genevisible; Q24255; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:FlyBase.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:UniProtKB.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0008057; P:eye pigment granule organization; IMP:UniProtKB.
DR GO; GO:0007455; P:eye-antennal disc morphogenesis; IMP:UniProtKB.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0008052; P:sensory organ boundary specification; IMP:UniProtKB.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Sensory transduction; Vision.
FT CHAIN 1..544
FT /note="Homeobox protein B-H1"
FT /id="PRO_0000048832"
FT DNA_BIND 299..358
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 53..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 92..179
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 471..544
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..133
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 154..168
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 276..295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 503..523
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 524..544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 45
FT /note="P -> A (in Ref. 1; AAA28382)"
FT /evidence="ECO:0000305"
FT CONFLICT 66
FT /note="A -> E (in Ref. 1; AAA28382)"
FT /evidence="ECO:0000305"
FT CONFLICT 218
FT /note="A -> R (in Ref. 1; AAA28382)"
FT /evidence="ECO:0000305"
FT CONFLICT 412
FT /note="Missing (in Ref. 1; AAA28382)"
FT /evidence="ECO:0000305"
FT CONFLICT 486
FT /note="V -> G (in Ref. 1; AAA28382)"
FT /evidence="ECO:0000305"
FT CONFLICT 504
FT /note="A -> R (in Ref. 1; AAA28382)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 544 AA; 56083 MW; BB536E3B00124926 CRC64;
MKDSMSILTQ TPSEPNAAHP QLHHHLSTLQ QQHHQHHLHY GLQPPAVAHS IHSTTTMSSG
GSTTTASGIG KPNRSRFMIN DILAGSAAAA FYKQQQHHQQ LHHHNNNNNS GSSGGSSPAH
SNNNNNINGD NCEASNVAGV GVLPSALHHP QPHPPTHPHT HPHALMHPHG KLGHFPPTAG
GNGLNVAQYA AAMQQHYAAA AAAAAARNNA AAAAAAAAAA AAAGVAAPPV DGGVDGGVGL
APPAGGDLDD SSDYHEENED CDSGNMDDHS VCSNGGKDDD GNSVKSGSTS DMSGLSKKQR
KARTAFTDHQ LQTLEKSFER QKYLSVQERQ ELAHKLDLSD CQVKTWYQNR RTKWKRQTAV
GLELLAEAGN FAAFQRLYGG SPYLGAWPYA AAAGAAHGAT PHTNIDIYYR QAAAAAAMQK
PLPYNLYAGV PSVGVGVGVG VGPAPFSHLS ASSSLSSLSS YYQSAAAAAS AANPGGPHPV
APPPSVGGGS PPSGLVKPIP AHSASASPPP RPPSTPSPTL NPGSPPGRSV DSCSQSDDED
QIQV
