BARK_DROME
ID BARK_DROME Reviewed; 3123 AA.
AC M9NDE3; Q9VQV1;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Protein bark beetle {ECO:0000303|PubMed:25704509};
DE AltName: Full=Protein anakonda {ECO:0000303|PubMed:25982676};
DE Flags: Precursor;
GN Name=bark {ECO:0000312|FlyBase:FBgn0031571};
GN Synonyms=aka {ECO:0000303|PubMed:25982676};
GN ORFNames=CG3921 {ECO:0000312|FlyBase:FBgn0031571};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=25704509; DOI=10.1016/j.ydbio.2015.02.008;
RA Hildebrandt A., Pflanz R., Behr M., Tarp T., Riedel D., Schuh R.;
RT "Bark beetle controls epithelial morphogenesis by septate junction
RT maturation in Drosophila.";
RL Dev. Biol. 400:237-247(2015).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION,
RP CLEAVAGE, DISRUPTION PHENOTYPE, MUTAGENESIS OF VAL-503; VAL-567; GLU-678
RP AND SER-680, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=25982676; DOI=10.1016/j.devcel.2015.03.023;
RA Byri S., Misra T., Syed Z.A., Baetz T., Shah J., Boril L., Glashauser J.,
RA Aegerter-Wilmsen T., Matzat T., Moussian B., Uv A., Luschnig S.;
RT "The triple-repeat protein anakonda controls epithelial tricellular
RT junction formation in Drosophila.";
RL Dev. Cell 33:535-548(2015).
CC -!- FUNCTION: Required for the maturation but not the establishment of
CC septate junctions in developing epithelial cells and is involved in
CC epithelial cell adhesion during septate junction maturation
CC (PubMed:25704509). Plays a role in the proper localization of the
CC septate junction core components pck/mega, kune, Nrx-IV and Nrg during
CC late embryogenesis (PubMed:25704509). Involved in the formation of
CC tricellular junctions which mediate cell contact where three epithelial
CC cells meet but not of bicellular junctions (PubMed:25982676). Required
CC for the accumulation of Gli at tricellular junctions.
CC {ECO:0000269|PubMed:25704509, ECO:0000269|PubMed:25982676}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000255}. Cell junction, septate junction
CC {ECO:0000269|PubMed:25704509}. Cell junction, adherens junction
CC {ECO:0000269|PubMed:25704509}. Cell junction, tight junction
CC {ECO:0000269|PubMed:25982676}. Note=Found at tricellular contacts.
CC {ECO:0000269|PubMed:25982676}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B {ECO:0000312|FlyBase:FBgn0031571};
CC IsoId=M9NDE3-1; Sequence=Displayed;
CC Name=A {ECO:0000312|FlyBase:FBgn0031571};
CC IsoId=M9NDE3-2; Sequence=VSP_057995;
CC -!- TISSUE SPECIFICITY: Expression detected in embryonic epithelia and
CC central nervous system (at protein level) (PubMed:25982676). First
CC detected during stage 13 in the tracheal system, the foregut, the
CC hindgut, the salivary glands and the epidermis (PubMed:25704509,
CC PubMed:25982676). Expression persists in these tissues until the end of
CC embryogenesis (PubMed:25704509). Expression in epithelia declines from
CC late stage 15 and expression appears in the central nervous system
CC during stage 16 (PubMed:25982676). {ECO:0000269|PubMed:25704509,
CC ECO:0000269|PubMed:25982676}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:25982676}.
CC -!- PTM: May be proteolytically cleaved in the extracellular domain.
CC {ECO:0000269|PubMed:25982676}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality. Mutant embryos establish
CC functional septate junctions but, due to rudimentary septae formation
CC during subsequent embryonic development, these become non-functional
CC (PubMed:25704509). Abnormal liquid clearance of tracheal tubes
CC (PubMed:25704509). Convoluted and elongated tracheal branches
CC (PubMed:25704509, PubMed:25982676). During late embryogenesis,
CC mislocalization of septate junction core components pck/mega, kune,
CC Nrx-IV and Nrg and impaired cell adhesion at the lateral cell membrane
CC (PubMed:25704509). Mislocalization of Fas3 in the epithelia of mutant
CC embryos and loss of accumulation of Gli at tricellular junctions
CC (PubMed:25982676). {ECO:0000269|PubMed:25704509,
CC ECO:0000269|PubMed:25982676}.
CC -!- MISCELLANEOUS: The name 'bark beetle' derives from the convoluted
CC tracheal branches seen in mutant embryos which resemble the tracks of
CC bark beetle larvae (PubMed:25704509). The name 'anakonda' is also based
CC on the convoluted tracheal tube phenotype of mutant embryos
CC (PubMed:25982676). {ECO:0000303|PubMed:25704509,
CC ECO:0000303|PubMed:25982676}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014134; AAF51061.1; -; Genomic_DNA.
DR EMBL; AE014134; AFH03540.1; -; Genomic_DNA.
DR EMBL; AE014134; AFH03541.1; -; Genomic_DNA.
DR RefSeq; NP_001245864.1; NM_001258935.3. [M9NDE3-1]
DR RefSeq; NP_001245865.1; NM_001258936.2. [M9NDE3-1]
DR RefSeq; NP_608808.1; NM_134964.4. [M9NDE3-2]
DR SMR; M9NDE3; -.
DR IntAct; M9NDE3; 3.
DR STRING; 7227.FBpp0297894; -.
DR GlyGen; M9NDE3; 38 sites.
DR PaxDb; M9NDE3; -.
DR PRIDE; M9NDE3; -.
DR EnsemblMetazoa; FBtr0077496; FBpp0077185; FBgn0031571. [M9NDE3-2]
DR EnsemblMetazoa; FBtr0307051; FBpp0297894; FBgn0031571. [M9NDE3-1]
DR EnsemblMetazoa; FBtr0307052; FBpp0297895; FBgn0031571. [M9NDE3-1]
DR GeneID; 33604; -.
DR KEGG; dme:Dmel_CG3921; -.
DR UCSC; CG3921-RA; d. melanogaster.
DR CTD; 33604; -.
DR FlyBase; FBgn0031571; bark.
DR VEuPathDB; VectorBase:FBgn0031571; -.
DR eggNOG; ENOG502QT9G; Eukaryota.
DR HOGENOM; CLU_000296_1_0_1; -.
DR OMA; MEFAPNV; -.
DR PhylomeDB; M9NDE3; -.
DR SignaLink; M9NDE3; -.
DR BioGRID-ORCS; 33604; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33604; -.
DR PRO; PR:M9NDE3; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0031571; Expressed in insect embryonic/larval digestive system and 30 other tissues.
DR Genevisible; Q9VQV1; DM.
DR GO; GO:0005912; C:adherens junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005923; C:bicellular tight junction; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016328; C:lateral plasma membrane; IDA:FlyBase.
DR GO; GO:0005918; C:septate junction; IDA:FlyBase.
DR GO; GO:0061689; C:tricellular tight junction; IDA:FlyBase.
DR GO; GO:0030246; F:carbohydrate binding; ISS:FlyBase.
DR GO; GO:0005044; F:scavenger receptor activity; IEA:InterPro.
DR GO; GO:0045217; P:cell-cell junction maintenance; IMP:FlyBase.
DR GO; GO:0035002; P:liquid clearance, open tracheal system; IMP:FlyBase.
DR GO; GO:0035159; P:regulation of tube length, open tracheal system; IMP:FlyBase.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IMP:FlyBase.
DR GO; GO:1904274; P:tricellular tight junction assembly; IMP:FlyBase.
DR CDD; cd00041; CUB; 1.
DR Gene3D; 2.60.120.290; -; 1.
DR Gene3D; 3.10.100.10; -; 1.
DR Gene3D; 3.10.250.10; -; 3.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR000859; CUB_dom.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR InterPro; IPR035914; Sperma_CUB_dom_sf.
DR InterPro; IPR001190; SRCR.
DR InterPro; IPR017448; SRCR-like_dom.
DR InterPro; IPR036772; SRCR-like_dom_sf.
DR Pfam; PF00530; SRCR; 3.
DR PRINTS; PR00258; SPERACTRCPTR.
DR SMART; SM00710; PbH1; 19.
DR SMART; SM00202; SR; 3.
DR SUPFAM; SSF49854; SSF49854; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR SUPFAM; SSF56487; SSF56487; 3.
DR PROSITE; PS00420; SRCR_1; 1.
DR PROSITE; PS50287; SRCR_2; 3.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell junction; Cell membrane; Disulfide bond;
KW Glycoprotein; Membrane; Reference proteome; Repeat; Signal; Tight junction;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..3123
FT /note="Protein bark beetle"
FT /id="PRO_5004101294"
FT TOPO_DOM 35..2714
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 2715..2735
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 2736..3123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT DOMAIN 191..295
FT /note="SRCR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT REPEAT 358..380
FT /note="PbH1 1"
FT /evidence="ECO:0000255"
FT REPEAT 382..404
FT /note="PbH1 2"
FT /evidence="ECO:0000255"
FT REPEAT 406..428
FT /note="PbH1 3"
FT /evidence="ECO:0000255"
FT DOMAIN 446..559
FT /note="CUB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00059"
FT REPEAT 562..584
FT /note="PbH1 4"
FT /evidence="ECO:0000255"
FT REPEAT 586..609
FT /note="PbH1 5"
FT /evidence="ECO:0000255"
FT REPEAT 611..633
FT /note="PbH1 6"
FT /evidence="ECO:0000255"
FT REPEAT 756..778
FT /note="PbH1 7"
FT /evidence="ECO:0000255"
FT REPEAT 789..809
FT /note="PbH1 8"
FT /evidence="ECO:0000255"
FT DOMAIN 1071..1175
FT /note="SRCR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT REPEAT 1219..1241
FT /note="PbH1 9"
FT /evidence="ECO:0000255"
FT REPEAT 1248..1270
FT /note="PbH1 10"
FT /evidence="ECO:0000255"
FT REPEAT 1451..1475
FT /note="PbH1 11"
FT /evidence="ECO:0000255"
FT REPEAT 1489..1511
FT /note="PbH1 12"
FT /evidence="ECO:0000255"
FT REPEAT 1553..1575
FT /note="PbH1 13"
FT /evidence="ECO:0000255"
FT REPEAT 1722..1744
FT /note="PbH1 14"
FT /evidence="ECO:0000255"
FT DOMAIN 1912..2037
FT /note="SRCR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT REPEAT 2104..2126
FT /note="PbH1 15"
FT /evidence="ECO:0000255"
FT REPEAT 2128..2150
FT /note="PbH1 16"
FT /evidence="ECO:0000255"
FT REPEAT 2337..2361
FT /note="PbH1 17"
FT /evidence="ECO:0000255"
FT REPEAT 2372..2393
FT /note="PbH1 18"
FT /evidence="ECO:0000255"
FT REPEAT 2401..2424
FT /note="PbH1 19"
FT /evidence="ECO:0000255"
FT REGION 83..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2766..2789
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2961..2983
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2996..3015
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3025..3123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 84..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2996..3013
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3061..3087
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3104..3123
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 221
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 377
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 389
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 403
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 498
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 523
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 615
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 620
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 630
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 639
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 658
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 672
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 702
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 709
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 834
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 900
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1040
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1375
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1489
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1520
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1529
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1584
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1593
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1614
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1883
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1920
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1940
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2231
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2251
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2314
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2357
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2459
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2536
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2546
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2566
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2596
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2636
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 216..284
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 231..294
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 262..272
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 446..474
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00059"
FT DISULFID 1096..1164
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 1109..1174
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 1144..1154
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 1950..2025
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 1963..2036
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT DISULFID 2000..2010
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00196"
FT VAR_SEQ 2539..2547
FT /note="ILSFDYENR -> M (in isoform A)"
FT /id="VSP_057995"
FT MUTAGEN 503
FT /note="V->E: In L224; embryonic lethality with excessively
FT elongated tracheal tubes."
FT /evidence="ECO:0000269|PubMed:25982676"
FT MUTAGEN 567
FT /note="V->E: In J55; embryonic lethality with excessively
FT elongated tracheal tubes."
FT /evidence="ECO:0000269|PubMed:25982676"
FT MUTAGEN 678
FT /note="E->K: In K93; embryonic lethality with excessively
FT elongated tracheal tubes."
FT /evidence="ECO:0000269|PubMed:25982676"
FT MUTAGEN 680
FT /note="S->L: In K104; embryonic lethality with excessively
FT elongated tracheal tubes."
FT /evidence="ECO:0000269|PubMed:25982676"
SQ SEQUENCE 3123 AA; 350461 MW; 3AB68B97DE35D73D CRC64;
MKLQHHKTNR QRISKPHRDP KWASICLWLL VTLAFSTHLA RSQESRQTED SKEVELLQDN
DIEFASLDGA SQLLPATRHS GADVTVAPQG STPSMTSSSS YTELQGGEIL SDRILRRSES
PYLARDDIEV LRGARLTIEP GVTIEFAPTK GLKINGVLQA VGTPTSRIVL KSQSNTANYK
LELPDDQEKG IRLVDGPTPV EGRLQLFHKG AWRSVCSNSR NWTLADYGVA CKQLGYRGGR
FWNWVERTPG YYPRLLYEQP KCKGGEGSLQ DCAWTSRQMG AGACDYHNDL GIQCLPVHSE
TLGHWRGIYF DNAPSTKALG RDNIVYAAQS ESRLKYVDII RAGSGAGFNA KSAVEVQGLP
PQMEHVVISH SAYTGFNSTR PWAGFQLQNV TVRKCNGIGV FVNSSQGAVQ LDGCSIVDNA
GDGIKYVGHD LRGTERKDRA SIYDFCTLPT TSGQTYPISL SFTQKYYAGS GKECGKYFFT
RPGYLLTLHF ENFVLMQNET ATVEIYDGAS TNDRLLFEWK ARNFTRPQSV TSTREKMFVR
IRADARQELN GFFRMTSGDS VAYDLKVSQS TVEDNGGRGV AIDNIRSKLH VHSSSVSGNG
HVAGVHVTSG AGDVNITSSN ISFNNGAGVN ITYYGGNRNI SRSALTANKG YGVATWLNQT
SDVNRMEYIP FNQTSVVEYS QIGGNLETGV FHGNFCRPIW VNITGNSFNG SQQNDIFIES
CYQATANGRP NMQLQLGHNQ FKYSQANSIY LSPALNLQGR IEYNMFRFGS YGCLFINNDY
IYPEFNYFPV KLIIQSNYFM RNSGVHVVSL GLSPYSRAEV QYILFTRNFV RGNNITEAFG
PLIAGSEGSD GAGRLNPRSR VAAPVVVGSS NVDIFRNILH NLDSMYEIGS QLTDQSKIIN
ATCNWLGHTD ENKIYARLFH RNDRYNLAKI NYLPYLLHSS NPGSTAMITV STVVPRFFHE
GSDVIGGEVD GQDMVPAGTY TVTKDINIRP GGKLILQPGT TLKFEPSVGM MVAGKLEARG
RRPDDILFTL KRETIMGESN DTETIDLDSE TEAIDMETEV IPADGVPRVP VRLVGGAGAN
EGRLQVYLKG RWGTVCDYGW NVLNAALVCH QLGYSLNPQD WRLLRSQLPN AGTSEDILMA
NVRCTLQDRD VTKCRAEYEF ENTCSHENDV GLRCYEGAWA GVRFSMLAER ADLQYVTVEK
AGLFDYTTNA FKPAVQMDHA RHNLENVRIV NNLQDGLGII YADIYAGKSV NNIKNSEFSG
NKGSGISLKQ LDFRVSGSII KDNKGSGVSH DAVISALDQK EIGGWFNMAT DFNSFDTDYD
PYLLPREISN IDLGTFEHKY IRTEELLGQN INRKIVVQCP AGYVIGIQLL NPIHNLSTES
INILNARTEN IRSDLWQVKR DLNVFPVTSS SYGIIIYYES GLQALGGAVL MLSTVTAPVQ
NIRNRIVSGA VPTLTIRSTK IQKNLRGITG IYYNRYIGDN GEYYLRKANE SIKLINSELS
YNEREAILIR SPFWDVISSN LSEVTLHVNG SLITQNGLGI RQMSKDLRSS NNLFHYVIQD
TTFEQNTHGG FQVSLPYVWQ YNENFTHSIY FGNSTWQRNR DFRISVSGHY TVFNITSNVF
RENNCPGALI SLDGMEKRLR FDNNRFESNN AKFVLLFKAD SLSEIIGQVP ASIEFNSFKG
NNIVTMTANY RNHYMKVARR IRKQHKIPTA VIRLDGVQNV RLYRNLIAEN EMDYNLVAGV
RSARLNNYFE ARENWWGTKD TAFIEAKIFD FDNWNDHADV IYQPFLIEDS YDASVSVVVP
FNQDQEIDLT NYKGGRVYKD LLLTKQSTPY YISSDITVMP GKTLTINHGV TMEFEPNVGI
LVLGTLVAIG YRESPIVMRP FRNATRESLI DVQPKKRALE DMSAPLTEFD SIRLCTSANN
CTGDADGLFG LNEGFLEYFN HTTLQWVPIC DSRFTERNAQ VVCRELGYDP LNVYYGHDRR
IEFHTNSLTR IWSWVQPLEC RGDEERMEDC AERLNGQLYG HRHECRWDDV FVFVSCNGIA
DDEVYWGGIR FANSKFEEIQ YEHRLHNTRS HARLPLRESQ LEFVRIEQAG ILHNHKAAAI
QAIHKNPSIT SVSIENSANH GINMIAPSGK LNLNHLNINN TLGTGISIVS LSGEGRDSDE
SSFTPLKKLD LPYKLFSLVD ICDPQKVLTI EERMLIYYKY DNNPVNCVKI FTSAFRAKPI
GFRLLQSNLF NHSKLYGRTD FIKLYDGDIY NVTATYLGKI ESDTDNQRSF FKTKGPTMSL
QLVASGAPET HGFIAEVVTV PISTLGQYRD ALHNITDTHI SGAIKGAVTY SSAGEVTPTL
TLIGNRIEKN CRQLYGNFST CTSALNLDVQ NMNSLYFMNN LITENQGGLR IRADSRGSAT
SLRGFVHHNL FMRNRNRPAL YVEGRQSSPY QEVELYRNYF AQNMAGYEDV IRLCQVVSNF
SYNYVHSNVG GRIMEVSGFE KVRLQIYQTT AHNGFYRNFA TNWMTRATIV AGTAGQQYVD
NIFENHENDY ELLTVNNSIL SFDYENRTFE TWSSKIDARH NYWSYNNTIS VQSRIRDKSD
DPMLLEVLAV PFQMNNETIL DGKCPPGWAL VHDTCFIYVG APMTFHEARD FCRSENSTMP
FIRTDKTTLW KYLQSQMRHL KYPDKVWIQD YNHIDRCTSF VFGEIEIEDC NKERGFICES
DPRVIIDPLS WRADIFAISI ISAFVLAIIL LILVAFCWFA KSKHRHTQRL QRRNSIRQSL
RSLNSIDPQG SLRRRPNYNM SSNGTLSKGQ DYKQMVANGS IDSMDKSVLS SEAGSFEGYE
QKPHYNEYVN QNALRPAHPA QDHQSHKVAT ISKASGHRAR AAAAAAAALE PDAFELSYRN
EGFRDNSTYG DNTRANSIST SVAEDTPIIH HTDQEIDEGG SDYYGNASTL PLRTEGGTPA
GRRGQPGLAF LSELKQNLPE YQRSSHSSFM PHRSSGDSLP FDQKLDQFNY STESSLYRPA
PAVPSSQQAT PADMRRPDSY YTAVRSSKAP VSHYRTPRPL AQPPAAPNVA PAGGPAQRRP
KTVYQTASEE SSPTTPSPLT NQYHRSKSEA LLETDFDGDG GSVGLQPLQT NGRSHSQPLE
TAM
