BARS_BACAM
ID BARS_BACAM Reviewed; 90 AA.
AC P11540;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Barstar;
DE AltName: Full=Ribonuclease inhibitor;
OS Bacillus amyloliquefaciens (Bacillus velezensis).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus amyloliquefaciens group.
OX NCBI_TaxID=1390;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3050134; DOI=10.1016/0022-2836(88)90568-2;
RA Hartley R.W.;
RT "Barnase and barstar. Expression of its cloned inhibitor permits expression
RT of a cloned ribonuclease.";
RL J. Mol. Biol. 202:913-915(1988).
RN [2]
RP REVIEW.
RX PubMed=2696173; DOI=10.1016/0968-0004(89)90104-7;
RA Hartley R.W.;
RT "Barnase and barstar: two small proteins to fold and fit together.";
RL Trends Biochem. Sci. 14:450-454(1989).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF COMPLEX WITH BARNASE.
RX PubMed=16100951; DOI=10.1016/0969-2126(93)90018-c;
RA Guillet V., Lapthorn A., Hartley R.W., Mauguen Y.;
RT "Recognition between a bacterial ribonuclease, barnase, and its natural
RT inhibitor, barstar.";
RL Structure 1:165-177(1993).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF COMPLEX WITH RNASE SA.
RX PubMed=9757110; DOI=10.1107/s0907444998004429;
RA Sevcik J., Urbanikova L., Dauter Z., Wilson K.S.;
RT "Recognition of RNase Sa by the inhibitor barstar: structure of the complex
RT at 1.7 A resolution.";
RL Acta Crystallogr. D 54:954-963(1998).
RN [5]
RP STRUCTURE BY NMR.
RX PubMed=8405454; DOI=10.1016/0014-5793(93)80489-h;
RA Lubienski M.J., Bycroft M., Jones D.N.M., Fersht A.R.;
RT "Assignment of the backbone 1H and 15N NMR resonances and secondary
RT structure characterization of barstar.";
RL FEBS Lett. 332:81-87(1993).
RN [6]
RP STRUCTURE BY NMR.
RX PubMed=8043574; DOI=10.1021/bi00196a003;
RA Lubienski M.J., Bycroft M., Freund S.M.V., Fersht A.R.;
RT "Three-dimensional solution structure and 13C assignments of barstar using
RT nuclear magnetic resonance spectroscopy.";
RL Biochemistry 33:8866-8877(1994).
CC -!- FUNCTION: Inhibitor of the ribonuclease barnase. Forms a one-to-one
CC non-covalent complex.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the barstar family. {ECO:0000305}.
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DR EMBL; X15545; CAA33551.1; -; Genomic_DNA.
DR PIR; S01373; S01373.
DR RefSeq; WP_013351425.1; NZ_VRTX01000005.1.
DR PDB; 1A19; X-ray; 2.76 A; A/B=1-90.
DR PDB; 1AB7; NMR; -; A=2-90.
DR PDB; 1AY7; X-ray; 1.70 A; B=2-90.
DR PDB; 1B27; X-ray; 2.10 A; D/E/F=1-90.
DR PDB; 1B2S; X-ray; 1.82 A; D/E/F=1-90.
DR PDB; 1B2U; X-ray; 2.10 A; D/E/F=1-90.
DR PDB; 1B3S; X-ray; 2.39 A; D/E/F=1-90.
DR PDB; 1BGS; X-ray; 2.60 A; E/F/G=2-90.
DR PDB; 1BRS; X-ray; 2.00 A; D/E/F=2-90.
DR PDB; 1BTA; NMR; -; A=2-90.
DR PDB; 1BTB; NMR; -; A=2-90.
DR PDB; 1L1K; NMR; -; A=2-21.
DR PDB; 1X1U; X-ray; 2.30 A; D/E/F=2-90.
DR PDB; 1X1W; X-ray; 2.10 A; D/E/F=1-90.
DR PDB; 1X1X; X-ray; 2.30 A; D/E/F=2-90.
DR PDB; 1X1Y; X-ray; 1.90 A; D/E/F=1-90.
DR PDB; 2HXX; X-ray; 2.00 A; A/B=2-90.
DR PDB; 2ZA4; X-ray; 1.58 A; B/D=1-90.
DR PDB; 3DA7; X-ray; 2.25 A; C/D/F/H=1-90.
DR PDB; 6PQK; X-ray; 1.20 A; B/D=1-90.
DR PDBsum; 1A19; -.
DR PDBsum; 1AB7; -.
DR PDBsum; 1AY7; -.
DR PDBsum; 1B27; -.
DR PDBsum; 1B2S; -.
DR PDBsum; 1B2U; -.
DR PDBsum; 1B3S; -.
DR PDBsum; 1BGS; -.
DR PDBsum; 1BRS; -.
DR PDBsum; 1BTA; -.
DR PDBsum; 1BTB; -.
DR PDBsum; 1L1K; -.
DR PDBsum; 1X1U; -.
DR PDBsum; 1X1W; -.
DR PDBsum; 1X1X; -.
DR PDBsum; 1X1Y; -.
DR PDBsum; 2HXX; -.
DR PDBsum; 2ZA4; -.
DR PDBsum; 3DA7; -.
DR PDBsum; 6PQK; -.
DR AlphaFoldDB; P11540; -.
DR BMRB; P11540; -.
DR SMR; P11540; -.
DR DIP; DIP-621N; -.
DR IntAct; P11540; 2.
DR STRING; 692420.BAMF_0800; -.
DR eggNOG; COG2732; Bacteria.
DR OMA; CDITIIL; -.
DR EvolutionaryTrace; P11540; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR Gene3D; 3.30.370.10; -; 1.
DR InterPro; IPR000468; Barstar.
DR InterPro; IPR035905; Barstar-like_sf.
DR Pfam; PF01337; Barstar; 1.
DR SUPFAM; SSF52038; SSF52038; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..90
FT /note="Barstar"
FT /id="PRO_0000064827"
FT STRAND 2..7
FT /evidence="ECO:0007829|PDB:6PQK"
FT HELIX 8..10
FT /evidence="ECO:0007829|PDB:6PQK"
FT HELIX 14..24
FT /evidence="ECO:0007829|PDB:6PQK"
FT HELIX 35..44
FT /evidence="ECO:0007829|PDB:6PQK"
FT STRAND 48..55
FT /evidence="ECO:0007829|PDB:6PQK"
FT HELIX 57..61
FT /evidence="ECO:0007829|PDB:6PQK"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:6PQK"
FT HELIX 68..80
FT /evidence="ECO:0007829|PDB:6PQK"
FT STRAND 85..90
FT /evidence="ECO:0007829|PDB:6PQK"
SQ SEQUENCE 90 AA; 10343 MW; 3A8E7A2348DEEC98 CRC64;
MKKAVINGEQ IRSISDLHQT LKKELALPEY YGENLDALWD CLTGWVEYPL VLEWRQFEQS
KQLTENGAES VLQVFREAKA EGCDITIILS