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BARS_BACAM
ID   BARS_BACAM              Reviewed;          90 AA.
AC   P11540;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Barstar;
DE   AltName: Full=Ribonuclease inhibitor;
OS   Bacillus amyloliquefaciens (Bacillus velezensis).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus amyloliquefaciens group.
OX   NCBI_TaxID=1390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3050134; DOI=10.1016/0022-2836(88)90568-2;
RA   Hartley R.W.;
RT   "Barnase and barstar. Expression of its cloned inhibitor permits expression
RT   of a cloned ribonuclease.";
RL   J. Mol. Biol. 202:913-915(1988).
RN   [2]
RP   REVIEW.
RX   PubMed=2696173; DOI=10.1016/0968-0004(89)90104-7;
RA   Hartley R.W.;
RT   "Barnase and barstar: two small proteins to fold and fit together.";
RL   Trends Biochem. Sci. 14:450-454(1989).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF COMPLEX WITH BARNASE.
RX   PubMed=16100951; DOI=10.1016/0969-2126(93)90018-c;
RA   Guillet V., Lapthorn A., Hartley R.W., Mauguen Y.;
RT   "Recognition between a bacterial ribonuclease, barnase, and its natural
RT   inhibitor, barstar.";
RL   Structure 1:165-177(1993).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF COMPLEX WITH RNASE SA.
RX   PubMed=9757110; DOI=10.1107/s0907444998004429;
RA   Sevcik J., Urbanikova L., Dauter Z., Wilson K.S.;
RT   "Recognition of RNase Sa by the inhibitor barstar: structure of the complex
RT   at 1.7 A resolution.";
RL   Acta Crystallogr. D 54:954-963(1998).
RN   [5]
RP   STRUCTURE BY NMR.
RX   PubMed=8405454; DOI=10.1016/0014-5793(93)80489-h;
RA   Lubienski M.J., Bycroft M., Jones D.N.M., Fersht A.R.;
RT   "Assignment of the backbone 1H and 15N NMR resonances and secondary
RT   structure characterization of barstar.";
RL   FEBS Lett. 332:81-87(1993).
RN   [6]
RP   STRUCTURE BY NMR.
RX   PubMed=8043574; DOI=10.1021/bi00196a003;
RA   Lubienski M.J., Bycroft M., Freund S.M.V., Fersht A.R.;
RT   "Three-dimensional solution structure and 13C assignments of barstar using
RT   nuclear magnetic resonance spectroscopy.";
RL   Biochemistry 33:8866-8877(1994).
CC   -!- FUNCTION: Inhibitor of the ribonuclease barnase. Forms a one-to-one
CC       non-covalent complex.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the barstar family. {ECO:0000305}.
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DR   EMBL; X15545; CAA33551.1; -; Genomic_DNA.
DR   PIR; S01373; S01373.
DR   RefSeq; WP_013351425.1; NZ_VRTX01000005.1.
DR   PDB; 1A19; X-ray; 2.76 A; A/B=1-90.
DR   PDB; 1AB7; NMR; -; A=2-90.
DR   PDB; 1AY7; X-ray; 1.70 A; B=2-90.
DR   PDB; 1B27; X-ray; 2.10 A; D/E/F=1-90.
DR   PDB; 1B2S; X-ray; 1.82 A; D/E/F=1-90.
DR   PDB; 1B2U; X-ray; 2.10 A; D/E/F=1-90.
DR   PDB; 1B3S; X-ray; 2.39 A; D/E/F=1-90.
DR   PDB; 1BGS; X-ray; 2.60 A; E/F/G=2-90.
DR   PDB; 1BRS; X-ray; 2.00 A; D/E/F=2-90.
DR   PDB; 1BTA; NMR; -; A=2-90.
DR   PDB; 1BTB; NMR; -; A=2-90.
DR   PDB; 1L1K; NMR; -; A=2-21.
DR   PDB; 1X1U; X-ray; 2.30 A; D/E/F=2-90.
DR   PDB; 1X1W; X-ray; 2.10 A; D/E/F=1-90.
DR   PDB; 1X1X; X-ray; 2.30 A; D/E/F=2-90.
DR   PDB; 1X1Y; X-ray; 1.90 A; D/E/F=1-90.
DR   PDB; 2HXX; X-ray; 2.00 A; A/B=2-90.
DR   PDB; 2ZA4; X-ray; 1.58 A; B/D=1-90.
DR   PDB; 3DA7; X-ray; 2.25 A; C/D/F/H=1-90.
DR   PDB; 6PQK; X-ray; 1.20 A; B/D=1-90.
DR   PDBsum; 1A19; -.
DR   PDBsum; 1AB7; -.
DR   PDBsum; 1AY7; -.
DR   PDBsum; 1B27; -.
DR   PDBsum; 1B2S; -.
DR   PDBsum; 1B2U; -.
DR   PDBsum; 1B3S; -.
DR   PDBsum; 1BGS; -.
DR   PDBsum; 1BRS; -.
DR   PDBsum; 1BTA; -.
DR   PDBsum; 1BTB; -.
DR   PDBsum; 1L1K; -.
DR   PDBsum; 1X1U; -.
DR   PDBsum; 1X1W; -.
DR   PDBsum; 1X1X; -.
DR   PDBsum; 1X1Y; -.
DR   PDBsum; 2HXX; -.
DR   PDBsum; 2ZA4; -.
DR   PDBsum; 3DA7; -.
DR   PDBsum; 6PQK; -.
DR   AlphaFoldDB; P11540; -.
DR   BMRB; P11540; -.
DR   SMR; P11540; -.
DR   DIP; DIP-621N; -.
DR   IntAct; P11540; 2.
DR   STRING; 692420.BAMF_0800; -.
DR   eggNOG; COG2732; Bacteria.
DR   OMA; CDITIIL; -.
DR   EvolutionaryTrace; P11540; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.30.370.10; -; 1.
DR   InterPro; IPR000468; Barstar.
DR   InterPro; IPR035905; Barstar-like_sf.
DR   Pfam; PF01337; Barstar; 1.
DR   SUPFAM; SSF52038; SSF52038; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm.
FT   INIT_MET        1
FT                   /note="Removed"
FT   CHAIN           2..90
FT                   /note="Barstar"
FT                   /id="PRO_0000064827"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   HELIX           8..10
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   HELIX           14..24
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   HELIX           35..44
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   STRAND          48..55
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   HELIX           57..61
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   STRAND          63..65
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   HELIX           68..80
FT                   /evidence="ECO:0007829|PDB:6PQK"
FT   STRAND          85..90
FT                   /evidence="ECO:0007829|PDB:6PQK"
SQ   SEQUENCE   90 AA;  10343 MW;  3A8E7A2348DEEC98 CRC64;
     MKKAVINGEQ IRSISDLHQT LKKELALPEY YGENLDALWD CLTGWVEYPL VLEWRQFEQS
     KQLTENGAES VLQVFREAKA EGCDITIILS
 
 
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