ID   BARW_HORVU              Reviewed;         125 AA.
AC   P28814;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Barwin;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   PROTEIN SEQUENCE, PYROGLUTAMATE FORMATION AT GLN-1, AND DISULFIDE BONDS.
RC   STRAIN=cv. Hiproly; TISSUE=Seed;
RX   PubMed=1390663; DOI=10.1021/bi00152a012;
RA   Svensson B., Svendsen I., Hoejrup P., Roepstorff P., Ludvigsen S.,
RA   Poulsen F.M.;
RT   "Primary structure of barwin: a barley seed protein closely related to the
RT   C-terminal domain of proteins encoded by wound-induced plant genes.";
RL   Biochemistry 31:8767-8770(1992).
RN   [2]
RP   STRUCTURE BY NMR.
RC   STRAIN=cv. Hiproly; TISSUE=Seed;
RX   PubMed=1390664; DOI=10.1021/bi00152a013;
RA   Ludvigsen S., Poulsen F.M.;
RT   "Secondary structure in solution of barwin from barley seed using 1H
RT   nuclear magnetic resonance spectroscopy.";
RL   Biochemistry 31:8771-8782(1992).
CC   -!- FUNCTION: May be involved in a defense mechanism. Probable plant
CC       lectin. Binds weakly a chitin analog.
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DR   PIR; A43474; A43474.
DR   PDB; 1BW3; NMR; -; A=2-125.
DR   PDB; 1BW4; NMR; -; A=2-125.
DR   PDBsum; 1BW3; -.
DR   PDBsum; 1BW4; -.
DR   AlphaFoldDB; P28814; -.
DR   SMR; P28814; -.
DR   PRIDE; P28814; -.
DR   EvolutionaryTrace; P28814; -.
DR   ExpressionAtlas; P28814; baseline and differential.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:InterPro.
DR   GO; GO:0050832; P:defense response to fungus; IEA:InterPro.
DR   Gene3D; 2.40.40.10; -; 1.
DR   InterPro; IPR018226; Barwin_CS.
DR   InterPro; IPR001153; Barwin_dom.
DR   InterPro; IPR044301; PR4.
DR   InterPro; IPR036908; RlpA-like_sf.
DR   PANTHER; PTHR46351; PTHR46351; 1.
DR   Pfam; PF00967; Barwin; 1.
DR   PRINTS; PR00602; BARWIN.
DR   SUPFAM; SSF50685; SSF50685; 1.
DR   PROSITE; PS00771; BARWIN_1; 1.
DR   PROSITE; PS00772; BARWIN_2; 1.
DR   PROSITE; PS51174; BARWIN_3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Lectin;
KW   Plant defense; Pyrrolidone carboxylic acid.
FT   CHAIN           1..125
FT                   /note="Barwin"
FT                   /id="PRO_0000164261"
FT   DOMAIN          1..125
FT                   /note="Barwin"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00527"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:1390663"
FT   DISULFID        31..63
FT                   /evidence="ECO:0000269|PubMed:1390663"
FT   DISULFID        52..86
FT                   /evidence="ECO:0000269|PubMed:1390663"
FT   DISULFID        66..123
FT                   /evidence="ECO:0000269|PubMed:1390663"
FT   STRAND          2..9
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   HELIX           16..18
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   TURN            25..27
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   HELIX           32..35
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   HELIX           40..45
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          49..51
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          53..55
FT                   /evidence="ECO:0007829|PDB:1BW4"
FT   HELIX           60..62
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          66..70
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   TURN            72..74
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          77..84
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          87..90
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          91..93
FT                   /evidence="ECO:0007829|PDB:1BW4"
FT   STRAND          95..98
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   HELIX           99..102
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   HELIX           107..111
FT                   /evidence="ECO:0007829|PDB:1BW3"
FT   STRAND          112..121
FT                   /evidence="ECO:0007829|PDB:1BW3"
SQ   SEQUENCE   125 AA;  13737 MW;  AA4227B7DCD070B4 CRC64;
     QQANDVRATY HYYRPAQNNW DLGAPAVSAY CATWDASKPL SWRSKYGWTA FCGPAGPRGQ
     AACGKCLRVT NPATGAQITA RIVDQCANGG LDLDWDTVFT KIDTNGIGYQ QGHLNVNYQF
     VDCRD