BARX2_MOUSE
ID BARX2_MOUSE Reviewed; 283 AA.
AC O08686; Q80ZF9; Q921G1;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Homeobox protein BarH-like 2;
GN Name=Barx2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-62.
RC STRAIN=129/SvJ;
RX PubMed=12486129; DOI=10.1074/jbc.m207617200;
RA Meech R., Makarenkova H., Edelman D.B., Jones F.S.;
RT "The homeodomain protein Barx2 promotes myogenic differentiation and is
RT regulated by myogenic regulatory factors.";
RL J. Biol. Chem. 278:8269-8278(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 7-283.
RC STRAIN=BALB/cJ; TISSUE=Embryo;
RX PubMed=9122247; DOI=10.1073/pnas.94.6.2632;
RA Jones F.S., Kioussi C., Copertino D.W., Kallunki P., Holst B.D.,
RA Edelman G.M.;
RT "Barx2, a new homeobox gene of the Bar class, is expressed in neural and
RT craniofacial structures during development.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:2632-2637(1997).
CC -!- FUNCTION: Transcription factor. Binds optimally to the DNA consensus
CC sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural
CC adhesion molecules such as L1 or Ng-CAM during embryonic development of
CC both the central and peripherical nervous system. May be involved in
CC controlling adhesive processes in keratinizing epithelia.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Nervous system, particularly in the telencephalon,
CC spinal cord, and dorsal root ganglia.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in small groups of cells
CC undergoing tissue remodeling, such as ectodermal cells within
CC indentations surrounding the eye and maxillo-nasal groove and in the
CC first branchial pouch, lung buds, precartilagenous condensations, and
CC mesenchyme of the limb. At 9.5 dpc, expressed only to head, prominent
CC in the region of telencephalon and mesencephalon, and concentrated in
CC cells along the dorsal midline. At 10.5 dpc, expression is moderated in
CC the most rostral regions of the head, but particularly prominent in a
CC lateral band of cells in the periocular region. At 11.5 dpc, detected
CC in the telencephalon, frontonasal region and limbs. At 12.5 dpc,
CC expressed in the telencephalon and hindbrain.
CC -!- SIMILARITY: Belongs to the BAR homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB53146.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH12684.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CT030724; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC012684; AAH12684.1; ALT_INIT; mRNA.
DR EMBL; AY188085; AAO25649.1; -; Genomic_DNA.
DR EMBL; L77900; AAB53146.1; ALT_INIT; mRNA.
DR CCDS; CCDS52750.1; -.
DR PIR; JC6307; JC6307.
DR RefSeq; NP_038828.2; NM_013800.2.
DR AlphaFoldDB; O08686; -.
DR SMR; O08686; -.
DR BioGRID; 198303; 7.
DR IntAct; O08686; 3.
DR STRING; 10090.ENSMUSP00000112314; -.
DR iPTMnet; O08686; -.
DR PhosphoSitePlus; O08686; -.
DR PaxDb; O08686; -.
DR PRIDE; O08686; -.
DR ProteomicsDB; 277163; -.
DR Antibodypedia; 19208; 85 antibodies from 18 providers.
DR DNASU; 12023; -.
DR Ensembl; ENSMUST00000116615; ENSMUSP00000112314; ENSMUSG00000032033.
DR GeneID; 12023; -.
DR KEGG; mmu:12023; -.
DR UCSC; uc009oru.1; mouse.
DR CTD; 8538; -.
DR MGI; MGI:109617; Barx2.
DR VEuPathDB; HostDB:ENSMUSG00000032033; -.
DR eggNOG; KOG0488; Eukaryota.
DR GeneTree; ENSGT00940000159663; -.
DR HOGENOM; CLU_090214_0_0_1; -.
DR InParanoid; O08686; -.
DR OMA; EIQLMGL; -.
DR OrthoDB; 1382174at2759; -.
DR PhylomeDB; O08686; -.
DR TreeFam; TF350735; -.
DR BioGRID-ORCS; 12023; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Barx2; mouse.
DR PRO; PR:O08686; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; O08686; protein.
DR Bgee; ENSMUSG00000032033; Expressed in lip and 109 other tissues.
DR Genevisible; O08686; MM.
DR GO; GO:0015629; C:actin cytoskeleton; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0001502; P:cartilage condensation; IDA:MGI.
DR GO; GO:0014902; P:myotube differentiation; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0035914; P:skeletal muscle cell differentiation; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IDA:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..283
FT /note="Homeobox protein BarH-like 2"
FT /id="PRO_0000048839"
FT DNA_BIND 139..198
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 107..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 198..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 205..227
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 73
FT /note="L -> P (in Ref. 4; AAB53146)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="A -> S (in Ref. 2; AAH12684)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="E -> EA (in Ref. 2; AAH12684)"
FT /evidence="ECO:0000305"
FT CONFLICT 244..273
FT /note="Missing (in Ref. 4; AAB53146)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 283 AA; 31534 MW; BCD8CC756B820953 CRC64;
MHCHAELRLS SPGQLKAARR RYKTFMIDEI LSKETCDYFE KLSLYSVCPS LVVRPKPLHS
CTGSPSLRAY PLLSVITRQP TVISHLVPTG SGLTPVLTRH PVAAAEAAAA AAETPGGEAL
ASSESETEQP TPRQKKPRRS RTIFTELQLM GLEKKFQKQK YLSTPDRLDL AQSLGLTQLQ
VKTWYQNRRM KWKKMVLKGG QEAPTKPKGR PKKNSIPTSE EIEAEEKMNS QAQSQELLES
SERQEEPCDT QEPKACLVPL EVAEPIHQPQ ELSEASSEPP PLS
