ID   BAS1_MAGO7              Reviewed;         115 AA.
AC   G5EHI7;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 34.
DE   RecName: Full=Biotrophy-associated secreted protein 1 {ECO:0000303|PubMed:19357089};
DE   Flags: Precursor;
GN   Name=BAS1 {ECO:0000303|PubMed:19357089}; ORFNames=MGG_04795;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, DISRUPTION
RP   PHENOTYPE, AND SUBCELLULAR LOCATION.
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=19357089; DOI=10.1105/tpc.107.055228;
RA   Mosquera G., Giraldo M.C., Khang C.H., Coughlan S., Valent B.;
RT   "Interaction transcriptome analysis identifies Magnaporthe oryzae BAS1-4 as
RT   Biotrophy-associated secreted proteins in rice blast disease.";
RL   Plant Cell 21:1273-1290(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20435900; DOI=10.1105/tpc.109.069666;
RA   Khang C.H., Berruyer R., Giraldo M.C., Kankanala P., Park S.Y., Czymmek K.,
RA   Kang S., Valent B.;
RT   "Translocation of Magnaporthe oryzae effectors into rice cells and their
RT   subsequent cell-to-cell movement.";
RL   Plant Cell 22:1388-1403(2010).
RN   [4]
RP   FUNCTION.
RX   PubMed=29551940; DOI=10.1016/j.sjbs.2017.11.032;
RA   Yang J., Liu L., Wang Y., Wang C., Yan J., Liu Y., Wang C., Li C.;
RT   "Overexpression of BAS1 in rice blast fungus can promote blast fungus
RT   growth, sporulation and virulence in planta.";
RL   Saudi J. Biol. Sci. 24:1884-1893(2017).
RN   [5]
RP   FUNCTION.
RX   PubMed=29931642; DOI=10.1007/s11356-018-2532-y;
RA   Yang J., Wang Y., Liu L., Liu L., Wang C., Wang C., Li C.;
RT   "Effects of exogenous salicylic acid and pH on pathogenicity of biotrophy-
RT   associated secreted protein 1 (BAS1)-overexpressing strain, Magnaporthe
RT   oryzae.";
RL   Environ. Sci. Pollut. Res. Int. 26:13725-13737(2019).
CC   -!- FUNCTION: Secreted effector involved in biotrophic colonization of
CC       plant cells (PubMed:19357089). Induces an early, basal defense response
CC       in susceptible rice, including rapid callose deposition and ROS
CC       production in leaves and calli (PubMed:29551940, PubMed:29931642).
CC       Promotes also sporulation and mycelia growth suggesting a role across
CC       the whole process of interaction, from the biotrophic phase to
CC       sporulation (PubMed:29551940). {ECO:0000269|PubMed:19357089,
CC       ECO:0000269|PubMed:29551940, ECO:0000269|PubMed:29931642}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19357089}. Host
CC       cytoplasm {ECO:0000269|PubMed:19357089, ECO:0000269|PubMed:20435900}.
CC       Note=Secreted into the biotrophic interfacial complexes (BICs).
CC       {ECO:0000269|PubMed:19357089, ECO:0000269|PubMed:20435900}.
CC   -!- INDUCTION: Expression is highly up-regulated in invasive hyphae.
CC       {ECO:0000269|PubMed:19357089}.
CC   -!- DISRUPTION PHENOTYPE: Leads to a slight decrease in lesion numbers and
CC       lesion sizes after host leaves infection.
CC       {ECO:0000269|PubMed:19357089}.
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DR   EMBL; FJ807764; ACQ73206.1; -; Genomic_DNA.
DR   EMBL; CM001231; EHA58149.1; -; Genomic_DNA.
DR   RefSeq; XP_003710761.1; XM_003710713.1.
DR   AlphaFoldDB; G5EHI7; -.
DR   STRING; 318829.MGG_04795T0; -.
DR   EnsemblFungi; MGG_04795T0; MGG_04795T0; MGG_04795.
DR   GeneID; 2677789; -.
DR   KEGG; mgr:MGG_04795; -.
DR   VEuPathDB; FungiDB:MGG_04795; -.
DR   HOGENOM; CLU_2109510_0_0_1; -.
DR   Proteomes; UP000009058; Chromosome 1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Host cytoplasm; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..115
FT                   /note="Biotrophy-associated secreted protein 1"
FT                   /id="PRO_5007915086"
FT   REGION          24..115
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        88..106
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   115 AA;  13048 MW;  79F4821A9458E4EB CRC64;
     MHVFNFAALF TVLATFTATA AAADQGSNTF DQRYQGYPWR PANGPIREEK QENVGHRRGG
     AEYFTSGSPS IAAEDYSAKN APSRFEQWKA QQKERAERKQ DRGLNGIRRV ENYYP