ID   BAS4_MAGO7              Reviewed;         102 AA.
AC   G5EI20;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Biotrophy-associated secreted protein 4 {ECO:0000303|PubMed:19357089};
DE   Flags: Precursor;
GN   Name=BAS4 {ECO:0000303|PubMed:19357089}; ORFNames=MGG_10914;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, FUNCTION, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=19357089; DOI=10.1105/tpc.107.055228;
RA   Mosquera G., Giraldo M.C., Khang C.H., Coughlan S., Valent B.;
RT   "Interaction transcriptome analysis identifies Magnaporthe oryzae BAS1-4 as
RT   Biotrophy-associated secreted proteins in rice blast disease.";
RL   Plant Cell 21:1273-1290(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=20435900; DOI=10.1105/tpc.109.069666;
RA   Khang C.H., Berruyer R., Giraldo M.C., Kankanala P., Park S.Y., Czymmek K.,
RA   Kang S., Valent B.;
RT   "Translocation of Magnaporthe oryzae effectors into rice cells and their
RT   subsequent cell-to-cell movement.";
RL   Plant Cell 22:1388-1403(2010).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=31049006; DOI=10.1016/j.sjbs.2019.01.003;
RA   Wang C., Liu Y., Liu L., Wang Y., Yan J., Wang C., Li C., Yang J.;
RT   "The biotrophy-associated secreted protein 4 (BAS4) participates in the
RT   transition of Magnaporthe oryzae from the biotrophic to the necrotrophic
RT   phase.";
RL   Saudi J. Biol. Sci. 26:795-807(2019).
CC   -!- FUNCTION: Secreted effector involved in biotrophic colonization of
CC       plant cells (PubMed:19357089). Participates in transition from the
CC       biotrophic to the necrotrophic phase of Magnaporthe oryzae
CC       (PubMed:31049006). Elicits rice basic defense responses during the
CC       early stage of interaction and promotes cell death in the late stage of
CC       compatible interaction (PubMed:19357089). {ECO:0000269|PubMed:19357089,
CC       ECO:0000269|PubMed:31049006}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19357089,
CC       ECO:0000269|PubMed:20435900}. Note=Localizes to the extra-invasive
CC       hyphal membrane (EIHM) matrix. {ECO:0000269|PubMed:19357089,
CC       ECO:0000269|PubMed:20435900}.
CC   -!- INDUCTION: Expression is highly up-regulated in invasive hyphae.
CC       {ECO:0000269|PubMed:19357089}.
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DR   EMBL; FJ807767; ACQ73209.1; -; Genomic_DNA.
DR   EMBL; CM001235; EHA48095.1; -; Genomic_DNA.
DR   RefSeq; XP_003717679.1; XM_003717631.1.
DR   AlphaFoldDB; G5EI20; -.
DR   STRING; 318829.MGG_10914T0; -.
DR   EnsemblFungi; MGG_10914T0; MGG_10914T0; MGG_10914.
DR   GeneID; 2677066; -.
DR   KEGG; mgr:MGG_10914; -.
DR   VEuPathDB; FungiDB:MGG_10914; -.
DR   HOGENOM; CLU_2278030_0_0_1; -.
DR   OrthoDB; 1928648at2759; -.
DR   Proteomes; UP000009058; Chromosome 5.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..102
FT                   /note="Biotrophy-associated secreted protein 4"
FT                   /id="PRO_5007915087"
FT   CARBOHYD        36
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   102 AA;  11088 MW;  D9FEFAD9B2B362FA CRC64;
     MQLSFSAIAI LLAFAVNHAT ADSHQNLVCV RTYPDNNSAE NLEATRCACD WLKKNGKCDD
     CTIWENRLCH SDAKSLDGNE FEDACVRKCP NLGATGSSIP PA