BASR_ECOLI
ID BASR_ECOLI Reviewed; 222 AA.
AC P30843; Q2M6J1; Q5MNC7; Q5MNC9; Q5MNE0; Q5MNE5; Q5MNF2; Q5MNH0;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Transcriptional regulatory protein BasR;
GN Name=basR; Synonyms=pmrA; OrderedLocusNames=b4113, JW4074;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8282725; DOI=10.1093/oxfordjournals.jbchem.a124180;
RA Nagasawa S., Ishige K., Mizuno T.;
RT "Novel members of the two-component signal transduction genes in
RT Escherichia coli.";
RL J. Biochem. 114:350-357(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND REGULATION BY PMRD.
RC STRAIN=ECOR 1, ECOR 10, ECOR 11, ECOR 12, ECOR 13, ECOR 14, ECOR 15,
RC ECOR 16, ECOR 17, ECOR 18, ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22,
RC ECOR 24, ECOR 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34,
RC ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR 41, ECOR 42,
RC ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47, ECOR 48, ECOR 5, ECOR 51,
RC ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6,
RC ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR 67,
RC ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72, ECOR 8, ECOR 9, and
RC O2:HN / ECOR 50 / P97 / UPEC;
RX PubMed=15569938; DOI=10.1073/pnas.0406038101;
RA Winfield M.D., Groisman E.A.;
RT "Phenotypic differences between Salmonella and Escherichia coli resulting
RT from the disparate regulation of homologous genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:17162-17167(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP PHOSPHORYLATION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=15522865; DOI=10.1074/jbc.m410104200;
RA Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.;
RT "Functional characterization in vitro of all two-component signal
RT transduction systems from Escherichia coli.";
RL J. Biol. Chem. 280:1448-1456(2005).
CC -!- FUNCTION: Member of the two-component regulatory system BasS/BasR. BasR
CC induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS
CC loci, all involved in resistance to polymyxin (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: The eptA-basRS operon is positively autoregulated by BasR
CC under high iron or aluminum concentration conditions. {ECO:0000250}.
CC -!- PTM: Phosphorylated by BasS. {ECO:0000269|PubMed:15522865}.
CC -!- MISCELLANEOUS: The activity of the BasR protein is in some natural
CC isolates, but not in strain K12, controlled at a post-transcriptional
CC level by the PmrD protein under low Mg(2+) concentration conditions.
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DR EMBL; D14055; BAA03143.1; -; Genomic_DNA.
DR EMBL; AY725270; AAV92717.1; -; Genomic_DNA.
DR EMBL; AY725271; AAV92718.1; -; Genomic_DNA.
DR EMBL; AY725272; AAV92719.1; -; Genomic_DNA.
DR EMBL; AY725273; AAV92720.1; -; Genomic_DNA.
DR EMBL; AY725274; AAV92721.1; -; Genomic_DNA.
DR EMBL; AY725275; AAV92722.1; -; Genomic_DNA.
DR EMBL; AY725276; AAV92723.1; -; Genomic_DNA.
DR EMBL; AY725277; AAV92724.1; -; Genomic_DNA.
DR EMBL; AY725278; AAV92725.1; -; Genomic_DNA.
DR EMBL; AY725279; AAV92726.1; -; Genomic_DNA.
DR EMBL; AY725280; AAV92727.1; -; Genomic_DNA.
DR EMBL; AY725281; AAV92728.1; -; Genomic_DNA.
DR EMBL; AY725282; AAV92729.1; -; Genomic_DNA.
DR EMBL; AY725283; AAV92730.1; -; Genomic_DNA.
DR EMBL; AY725284; AAV92731.1; -; Genomic_DNA.
DR EMBL; AY725285; AAV92732.1; -; Genomic_DNA.
DR EMBL; AY725286; AAV92733.1; -; Genomic_DNA.
DR EMBL; AY725287; AAV92734.1; -; Genomic_DNA.
DR EMBL; AY725288; AAV92735.1; -; Genomic_DNA.
DR EMBL; AY725289; AAV92736.1; -; Genomic_DNA.
DR EMBL; AY725290; AAV92737.1; -; Genomic_DNA.
DR EMBL; AY725291; AAV92738.1; -; Genomic_DNA.
DR EMBL; AY725292; AAV92739.1; -; Genomic_DNA.
DR EMBL; AY725293; AAV92740.1; -; Genomic_DNA.
DR EMBL; AY725294; AAV92741.1; -; Genomic_DNA.
DR EMBL; AY725295; AAV92742.1; -; Genomic_DNA.
DR EMBL; AY725296; AAV92743.1; -; Genomic_DNA.
DR EMBL; AY725297; AAV92744.1; -; Genomic_DNA.
DR EMBL; AY725298; AAV92745.1; -; Genomic_DNA.
DR EMBL; AY725299; AAV92746.1; -; Genomic_DNA.
DR EMBL; AY725300; AAV92747.1; -; Genomic_DNA.
DR EMBL; AY725301; AAV92748.1; -; Genomic_DNA.
DR EMBL; AY725302; AAV92749.1; -; Genomic_DNA.
DR EMBL; AY725303; AAV92750.1; -; Genomic_DNA.
DR EMBL; AY725304; AAV92751.1; -; Genomic_DNA.
DR EMBL; AY725305; AAV92752.1; -; Genomic_DNA.
DR EMBL; AY725306; AAV92753.1; -; Genomic_DNA.
DR EMBL; AY725307; AAV92754.1; -; Genomic_DNA.
DR EMBL; AY725308; AAV92755.1; -; Genomic_DNA.
DR EMBL; AY725309; AAV92756.1; -; Genomic_DNA.
DR EMBL; AY725310; AAV92757.1; -; Genomic_DNA.
DR EMBL; AY725311; AAV92758.1; -; Genomic_DNA.
DR EMBL; AY725312; AAV92759.1; -; Genomic_DNA.
DR EMBL; AY725313; AAV92760.1; -; Genomic_DNA.
DR EMBL; AY725314; AAV92761.1; -; Genomic_DNA.
DR EMBL; AY725315; AAV92762.1; -; Genomic_DNA.
DR EMBL; AY725316; AAV92763.1; -; Genomic_DNA.
DR EMBL; AY725317; AAV92764.1; -; Genomic_DNA.
DR EMBL; AY725318; AAV92765.1; -; Genomic_DNA.
DR EMBL; AY725319; AAV92766.1; -; Genomic_DNA.
DR EMBL; AY725320; AAV92767.1; -; Genomic_DNA.
DR EMBL; AY725321; AAV92768.1; -; Genomic_DNA.
DR EMBL; AY725322; AAV92769.1; -; Genomic_DNA.
DR EMBL; AY725323; AAV92770.1; -; Genomic_DNA.
DR EMBL; AY725324; AAV92771.1; -; Genomic_DNA.
DR EMBL; AY725325; AAV92772.1; -; Genomic_DNA.
DR EMBL; AY725326; AAV92773.1; -; Genomic_DNA.
DR EMBL; AY725327; AAV92774.1; -; Genomic_DNA.
DR EMBL; AY725328; AAV92775.1; -; Genomic_DNA.
DR EMBL; AY725329; AAV92776.1; -; Genomic_DNA.
DR EMBL; AY725330; AAV92777.1; -; Genomic_DNA.
DR EMBL; AY725331; AAV92778.1; -; Genomic_DNA.
DR EMBL; AY725332; AAV92779.1; -; Genomic_DNA.
DR EMBL; AY725333; AAV92780.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97012.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77074.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78115.1; -; Genomic_DNA.
DR PIR; H65220; H65220.
DR RefSeq; NP_418537.1; NC_000913.3.
DR RefSeq; WP_000697919.1; NZ_STEB01000014.1.
DR RefSeq; WP_000697926.1; NZ_LN832404.1.
DR AlphaFoldDB; P30843; -.
DR SMR; P30843; -.
DR BioGRID; 4263083; 7.
DR DIP; DIP-9201N; -.
DR IntAct; P30843; 5.
DR STRING; 511145.b4113; -.
DR jPOST; P30843; -.
DR PaxDb; P30843; -.
DR PRIDE; P30843; -.
DR EnsemblBacteria; AAC77074; AAC77074; b4113.
DR EnsemblBacteria; BAE78115; BAE78115; BAE78115.
DR GeneID; 948631; -.
DR KEGG; ecj:JW4074; -.
DR KEGG; eco:b4113; -.
DR PATRIC; fig|1411691.4.peg.2587; -.
DR EchoBASE; EB1572; -.
DR eggNOG; COG0745; Bacteria.
DR HOGENOM; CLU_000445_30_1_6; -.
DR InParanoid; P30843; -.
DR OMA; LMREVWD; -.
DR PhylomeDB; P30843; -.
DR BioCyc; EcoCyc:BASR-MON; -.
DR PRO; PR:P30843; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR GO; GO:0000156; F:phosphorelay response regulator activity; IDA:EcoCyc.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0010041; P:response to iron(III) ion; IMP:EcoCyc.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 1: Evidence at protein level;
KW Activator; Antibiotic resistance; Cytoplasm; DNA-binding; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..222
FT /note="Transcriptional regulatory protein BasR"
FT /id="PRO_0000081023"
FT DOMAIN 2..116
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 124..218
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 51
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT VARIANT 29
FT /note="S -> G (in strain: ECOR 1, ECOR 10, ECOR 11, ECOR
FT 12, ECOR 13, ECOR 14, ECOR 15, ECOR 16, ECOR 17, ECOR 18,
FT ECOR 19, ECOR 2, ECOR 20, ECOR 21, ECOR 22, ECOR 24, ECOR
FT 25, ECOR 26, ECOR 27, ECOR 3, ECOR 30, ECOR 33, ECOR 34,
FT ECOR 35, ECOR 36, ECOR 37, ECOR 38, ECOR 4, ECOR 40, ECOR
FT 41, ECOR 42, ECOR 43, ECOR 44, ECOR 45, ECOR 46, ECOR 47,
FT ECOR 48, ECOR 5, ECOR 50, ECOR 51, ECOR 52, ECOR 53, ECOR
FT 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 6, ECOR 60,
FT ECOR 61, ECOR 62, ECOR 63, ECOR 64, ECOR 65, ECOR 66, ECOR
FT 67, ECOR 68, ECOR 69, ECOR 7, ECOR 70, ECOR 71, ECOR 72,
FT ECOR 8 and ECOR 9)"
FT VARIANT 31
FT /note="T -> S (in strain: ECOR 35, ECOR 36, ECOR 38, ECOR
FT 40, ECOR 41, ECOR 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55,
FT ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR
FT 63 and ECOR 64, ECOR 65 and ECOR 66)"
FT VARIANT 128
FT /note="I -> N (in strain: ECOR 51, ECOR 52, ECOR 53, ECOR
FT 54, ECOR 55, ECOR 56, ECOR 57, ECOR 59, ECOR 60, ECOR 61,
FT ECOR 62, ECOR 63 and ECOR 64)"
FT VARIANT 129
FT /note="V -> L (in strain: ECOR 19)"
FT VARIANT 144
FT /note="G -> S (in strain: ECOR 44, ECOR 48, ECOR 50, ECOR
FT 51, ECOR 52, ECOR 53, ECOR 54, ECOR 55, ECOR 56, ECOR 57,
FT ECOR 59, ECOR 60, ECOR 61, ECOR 62, ECOR 63, ECOR 64 and
FT ECOR 72)"
FT VARIANT 207
FT /note="R -> P (in strain: ECOR 27)"
FT VARIANT 214
FT /note="Y -> F (in strain: ECOR 27)"
FT CONFLICT 29
FT /note="S -> T (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 222 AA; 25031 MW; 8588F9F23A1B8625 CRC64;
MKILIVEDDT LLLQGLILAA QTEGYACDSV TTARMAEQSL EAGHYSLVVL DLGLPDEDGL
HFLARIRQKK YTLPVLILTA RDTLTDKIAG LDVGADDYLV KPFALEELHA RIRALLRRHN
NQGESELIVG NLTLNMGRRQ VWMGGEELIL TPKEYALLSR LMLKAGSPVH REILYNDIYN
WDNEPSTNTL EVHIHNLRDK VGKARIRTVR GFGYMLVANE EN
