BASS2_ARATH
ID BASS2_ARATH Reviewed; 409 AA.
AC Q1EBV7; O81017; Q84WD8; Q8S2V7;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Sodium/pyruvate cotransporter BASS2, chloroplastic;
DE AltName: Full=Bile acid transporter 1;
DE AltName: Full=Bile acid-sodium symporter family protein 2;
DE Flags: Precursor;
GN Name=BASS2; Synonyms=BAT1, SBF1; OrderedLocusNames=At2g26900;
GN ORFNames=F12C20.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12177140; DOI=10.1093/jxb/erf053;
RA Rzewuski G., Sauter M.;
RT "The novel rice (Oryza sativa L.) gene OsSbf1 encodes a putative member of
RT the Na+/bile acid symporter family.";
RL J. Exp. Bot. 53:1991-1993(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-409.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP ACETYLATION AT ALA-75, CLEAVAGE OF TRANSIT PEPTIDE AFTER LYS-74,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Wassilewskija;
RX PubMed=12766230; DOI=10.1074/mcp.m300030-mcp200;
RA Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M.,
RA Garin J., Joyard J., Rolland N.;
RT "Proteomics of the chloroplast envelope membranes from Arabidopsis
RT thaliana.";
RL Mol. Cell. Proteomics 2:325-345(2003).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=19542295; DOI=10.1105/tpc.109.066399;
RA Gigolashvili T., Yatusevich R., Rollwitz I., Humphry M., Gershenzon J.,
RA Fluegge U.-I.;
RT "The plastidic bile acid transporter 5 is required for the biosynthesis of
RT methionine-derived glucosinolates in Arabidopsis thaliana.";
RL Plant Cell 21:1813-1829(2009).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21866161; DOI=10.1038/nature10250;
RA Furumoto T., Yamaguchi T., Ohshima-Ichie Y., Nakamura M.,
RA Tsuchida-Iwata Y., Shimamura M., Ohnishi J., Hata S., Gowik U.,
RA Westhoff P., Braeutigam A., Weber A.P., Izui K.;
RT "A plastidial sodium-dependent pyruvate transporter.";
RL Nature 476:472-475(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-75, CLEAVAGE OF TRANSIT PEPTIDE
RP [LARGE SCALE ANALYSIS] AFTER LYS-74, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Sodium-coupled metabolite transporter that plays a crucial
CC role in pyruvate transport across the chloroplast envelope. Is required
CC for sodium-coupled pyruvate import into plastids.
CC {ECO:0000269|PubMed:21866161}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}. Plastid, chloroplast envelope {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in developing leaves.
CC {ECO:0000269|PubMed:21866161}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but plants show increased sensitivity to mevastatin, an
CC inhibitor of cytosolic isopentenyl diphosphate biosynthesis.
CC Chloroplasts isolated from mutant plants lack sodium-dependent pyruvate
CC uptake activity. {ECO:0000269|PubMed:21866161}.
CC -!- SIMILARITY: Belongs to the bile acid:sodium symporter (BASS) (TC
CC 2.A.28) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC32250.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAO41972.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF498303; AAM18095.1; -; mRNA.
DR EMBL; AC005168; AAC32250.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07905.1; -; Genomic_DNA.
DR EMBL; BT025977; ABG25066.1; -; mRNA.
DR EMBL; BT003925; AAO41972.1; ALT_INIT; mRNA.
DR PIR; T02645; T02645.
DR RefSeq; NP_850089.1; NM_179758.4.
DR AlphaFoldDB; Q1EBV7; -.
DR SMR; Q1EBV7; -.
DR BioGRID; 2583; 36.
DR IntAct; Q1EBV7; 36.
DR STRING; 3702.AT2G26900.1; -.
DR TCDB; 2.A.28.2.3; the bile acid:na(+) symporter (bass) family.
DR iPTMnet; Q1EBV7; -.
DR PaxDb; Q1EBV7; -.
DR PRIDE; Q1EBV7; -.
DR ProteomicsDB; 240815; -.
DR EnsemblPlants; AT2G26900.1; AT2G26900.1; AT2G26900.
DR GeneID; 817231; -.
DR Gramene; AT2G26900.1; AT2G26900.1; AT2G26900.
DR KEGG; ath:AT2G26900; -.
DR Araport; AT2G26900; -.
DR TAIR; locus:2039533; AT2G26900.
DR eggNOG; KOG2718; Eukaryota.
DR HOGENOM; CLU_034788_1_2_1; -.
DR InParanoid; Q1EBV7; -.
DR OMA; QFTIMPL; -.
DR OrthoDB; 1148347at2759; -.
DR PhylomeDB; Q1EBV7; -.
DR PRO; PR:Q1EBV7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q1EBV7; baseline and differential.
DR Genevisible; Q1EBV7; AT.
DR GO; GO:0009941; C:chloroplast envelope; IDA:UniProtKB.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0006849; P:plasma membrane pyruvate transport; IDA:UniProtKB.
DR Gene3D; 1.20.1530.20; -; 1.
DR InterPro; IPR002657; BilAc:Na_symport/Acr3.
DR InterPro; IPR004710; Bilac:Na_transpt.
DR InterPro; IPR038770; Na+/solute_symporter_sf.
DR PANTHER; PTHR10361; PTHR10361; 1.
DR Pfam; PF01758; SBF; 1.
DR TIGRFAMs; TIGR00841; bass; 1.
PE 1: Evidence at protein level;
KW Acetylation; Chloroplast; Membrane; Plastid; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..74
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:12766230,
FT ECO:0007744|PubMed:22223895"
FT CHAIN 75..409
FT /note="Sodium/pyruvate cotransporter BASS2, chloroplastic"
FT /id="PRO_0000418603"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 75
FT /note="N-acetylalanine"
FT /evidence="ECO:0000269|PubMed:12766230,
FT ECO:0007744|PubMed:22223895"
FT CONFLICT 132
FT /note="T -> S (in Ref. 1; AAM18095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 409 AA; 43608 MW; BBD40718551A2922 CRC64;
MASISRILPT DGRLSQCRIN TSWVPSTTRT QTHLDFPKLV SVSNSGISLR IQNSKPISPV
FALEATSSRR VVCKAAAGVS GDLPESTPKE LSQYEKIIEL LTTLFPLWVI LGTLVGIFKP
SLVTWLETDL FTLGLGFLML SMGLTLTFED FRRCLRNPWT VGVGFLAQYM IKPILGFLIA
MTLKLSAPLA TGLILVSCCP GGQASNVATY ISKGNVALSV LMTTCSTIGA IIMTPLLTKL
LAGQLVPVDA AGLALSTFQV VLVPTIIGVL ANEFFPKFTS KIITVTPLIG VILTTLLCAS
PIGQVADVLK TQGAQLILPV ALLHAAAFAI GYWISKFSFG ESTSRTISIE CGMQSSALGF
LLAQKHFTNP LVAVPSAVSV VCMALGGSGL AVFWRNLPIP ADDKDDFKE
