BAS_BRUGY
ID BAS_BRUGY Reviewed; 759 AA.
AC A8CDT2;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Beta-amyrin synthase;
DE Short=BgbAS;
DE EC=5.4.99.39;
GN Name=BAS;
OS Bruguiera gymnorhiza (Burma mangrove) (Rhizophora gymnorhiza).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Rhizophoraceae; Bruguiera.
OX NCBI_TaxID=39984;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC TISSUE=Leaf;
RX PubMed=17803686; DOI=10.1111/j.1742-4658.2007.06025.x;
RA Basyuni M., Oku H., Tsujimoto E., Kinjo K., Baba S., Takara K.;
RT "Triterpene synthases from the Okinawan mangrove tribe, Rhizophoraceae.";
RL FEBS J. 274:5028-5042(2007).
CC -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of beta-
CC amyrin. {ECO:0000269|PubMed:17803686}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = beta-amyrin; Xref=Rhea:RHEA:31007,
CC ChEBI:CHEBI:10352, ChEBI:CHEBI:15441; EC=5.4.99.39;
CC Evidence={ECO:0000269|PubMed:17803686};
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; AB289585; BAF80443.1; -; mRNA.
DR AlphaFoldDB; A8CDT2; -.
DR SMR; A8CDT2; -.
DR KEGG; ag:BAF80443; -.
DR BioCyc; MetaCyc:MON-14451; -.
DR BRENDA; 5.4.99.39; 1002.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0042300; F:beta-amyrin synthase activity; IDA:UniProtKB.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
PE 1: Evidence at protein level;
KW Isomerase; Repeat.
FT CHAIN 1..759
FT /note="Beta-amyrin synthase"
FT /id="PRO_0000412989"
FT REPEAT 148..189
FT /note="PFTB 1"
FT REPEAT 514..559
FT /note="PFTB 2"
FT REPEAT 591..631
FT /note="PFTB 3"
FT REPEAT 640..681
FT /note="PFTB 4"
FT ACT_SITE 485
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 759 AA; 87665 MW; 9335B3C84943318B CRC64;
MWRIKIAEGG KDPYLYSTNN YVGRQTWEFD PDAGTPEERA EVEEARQNFY KNRYQVKPCG
DLLWRLQFLG EKNFEQTIPQ VRIEEGEGIT YEKATRALRR TVQFFSALQA SDGHWPAEIA
GPLFFLPPLV MCVYITGHLD AVFPAEHRKE ILRYIYYHQN EDGGWGLHIE GHSTMFCTAL
NYICMRIIGE GPNGGQDDAC ARARKWIHDH GSVTNIPSWG KTWLSILGVY DWSGSNPMPP
EFWMLPSFLP MHPAKMWCYC RMVYMPMSYL YGKRFVGPIT PLIQQLREEL FTQPYDQINW
KKTRHQCAPE DLYYPHPFVQ DLIWDCLYIF TEPLLTRWPL NEIIRKKALE VTMKHIHYED
ESSRYITIGC VEKVLCMLAC WVEDPNGDYF KKHLARIPDY IWVAEDGMKM QSFGSQEWDT
GFAIQALLAT NLTDEIGDVL RRGHDFIKKS QVRDNPSGDF KSMYRHISKG SWTFSDQDHG
WQVSDCTAEG LKCCLLFSMM PPEIVGEHMV PERLYDSVNV LLSLQSKNGG LSAWEPAGAQ
EWLELLNPTE FFADIVIEHE YVECTSSAIH ALVLFKKLYP GHRKKEIDNF IVNAVRYLES
IQTSDGGWYG NWGVCFTYGT WFALGGLAAA GKTYNNCLAM RKAVDFLLRI QRDNGGWGES
YLSCPEKRYV PLEGNRSNLV HTAWALMALI HAGQMDRDPT PLHRAARLMI NSQLEDGDFP
QQEITGVFMK NCMLHYAAYR NIYPLWALAE YRRRVPLPS