RS8_BACSU
ID RS8_BACSU Reviewed; 132 AA.
AC P12879; P70968;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=30S ribosomal protein S8 {ECO:0000255|HAMAP-Rule:MF_01302};
DE Short=BS8;
GN Name=rpsH {ECO:0000255|HAMAP-Rule:MF_01302}; OrderedLocusNames=BSU01300;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8635744; DOI=10.1016/0378-1119(95)00757-1;
RA Suh J.-W., Boylan S.A., Oh S.H., Price C.W.;
RT "Genetic and transcriptional organization of the Bacillus subtilis spc-
RT alpha region.";
RL Gene 169:17-23(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969501; DOI=10.1099/13500872-142-11-3039;
RA Yasumoto K., Liu H., Jeong S.M., Ohashi Y., Kakinuma S., Tanaka K.,
RA Kawamura F., Yoshikawa H., Takahashi H.;
RT "Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus
RT subtilis chromosome.";
RL Microbiology 142:3039-3046(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP SEQUENCE REVISION TO 37 AND 63.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
RC STRAIN=168;
RX PubMed=2508062; DOI=10.1093/nar/17.18.7469;
RA Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M.;
RT "Cloning and analysis of the spc ribosomal protein operon of Bacillus
RT subtilis: comparison with the spc operon of Escherichia coli.";
RL Nucleic Acids Res. 17:7469-7486(1989).
RN [6]
RP PROTEIN SEQUENCE OF 2-31.
RX PubMed=6806564; DOI=10.1007/bf00330792;
RA Higo K., Otaka E., Osawa S.;
RT "Purification and characterization of 30S ribosomal proteins from Bacillus
RT subtilis: correlation to Escherichia coli 30S proteins.";
RL Mol. Gen. Genet. 185:239-244(1982).
RN [7] {ECO:0007744|PDB:6HA1, ECO:0007744|PDB:6HA8}
RP STRUCTURE BY ELECTRON MICROSCOPY (3.10 ANGSTROMS) OF 1-132 WITH AND WITHOUT
RP VIRGINIAMYCIN M, AND SUBUNIT.
RX PubMed=30126986; DOI=10.1073/pnas.1808535115;
RA Crowe-McAuliffe C., Graf M., Huter P., Takada H., Abdelshahid M.,
RA Novacek J., Murina V., Atkinson G.C., Hauryliuk V., Wilson D.N.;
RT "Structural basis for antibiotic resistance mediated by the Bacillus
RT subtilis ABCF ATPase VmlR.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:8978-8983(2018).
CC -!- FUNCTION: One of the primary rRNA binding proteins, it binds directly
CC to 16S rRNA central domain where it helps coordinate assembly of the
CC platform of the 30S subunit. {ECO:0000255|HAMAP-Rule:MF_01302}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit (PubMed:30126986). Contacts
CC proteins S5 and S12 (By similarity). {ECO:0000255|HAMAP-Rule:MF_01302,
CC ECO:0000269|PubMed:30126986}.
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS8 family.
CC {ECO:0000255|HAMAP-Rule:MF_01302}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L47971; AAB06813.1; -; Genomic_DNA.
DR EMBL; D64125; BAA10980.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11906.2; -; Genomic_DNA.
DR EMBL; X15664; CAA33705.1; -; Genomic_DNA.
DR PIR; G69699; G69699.
DR RefSeq; NP_388011.2; NC_000964.3.
DR RefSeq; WP_003241998.1; NZ_JNCM01000029.1.
DR PDB; 3J9W; EM; 3.90 A; AH=1-132.
DR PDB; 5NJT; EM; 3.80 A; H=2-132.
DR PDB; 6HA1; EM; 3.10 A; h=1-132.
DR PDB; 6HA8; EM; 3.50 A; h=1-132.
DR PDB; 6HTQ; EM; 4.50 A; h=2-132.
DR PDB; 7O5B; EM; 3.33 A; H=1-132.
DR PDB; 7QV1; EM; 3.50 A; h=1-132.
DR PDB; 7QV2; EM; 3.50 A; h=1-132.
DR PDB; 7QV3; EM; 5.14 A; h=1-132.
DR PDBsum; 3J9W; -.
DR PDBsum; 5NJT; -.
DR PDBsum; 6HA1; -.
DR PDBsum; 6HA8; -.
DR PDBsum; 6HTQ; -.
DR PDBsum; 7O5B; -.
DR PDBsum; 7QV1; -.
DR PDBsum; 7QV2; -.
DR PDBsum; 7QV3; -.
DR AlphaFoldDB; P12879; -.
DR SMR; P12879; -.
DR STRING; 224308.BSU01300; -.
DR jPOST; P12879; -.
DR PaxDb; P12879; -.
DR PRIDE; P12879; -.
DR EnsemblBacteria; CAB11906; CAB11906; BSU_01300.
DR GeneID; 936328; -.
DR KEGG; bsu:BSU01300; -.
DR PATRIC; fig|224308.179.peg.133; -.
DR eggNOG; COG0096; Bacteria.
DR InParanoid; P12879; -.
DR OMA; FDQGYIL; -.
DR PhylomeDB; P12879; -.
DR BioCyc; BSUB:BSU01300-MON; -.
DR PRO; PR:P12879; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003735; F:structural constituent of ribosome; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01302_B; Ribosomal_S8_B; 1.
DR InterPro; IPR000630; Ribosomal_S8.
DR InterPro; IPR035987; Ribosomal_S8_sf.
DR PANTHER; PTHR11758; PTHR11758; 1.
DR Pfam; PF00410; Ribosomal_S8; 1.
DR SUPFAM; SSF56047; SSF56047; 1.
DR PROSITE; PS00053; RIBOSOMAL_S8; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:6806564"
FT CHAIN 2..132
FT /note="30S ribosomal protein S8"
FT /id="PRO_0000126367"
FT CONFLICT 18..19
FT /note="NM -> DN (in Ref. 6; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 30
FT /note="S -> B (in Ref. 6; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 37
FT /note="A -> P (in Ref. 2; BAA10980)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="F -> S (in Ref. 2; BAA10980)"
FT /evidence="ECO:0000305"
FT CONFLICT 131..132
FT /note="Missing (in Ref. 2; BAA10980)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 132 AA; 14877 MW; B47FA5DAD9F2F055 CRC64;
MVMTDPIADM LTRIRNANMV RHEKLEIPAS KLKREIAEIL KREGFIRDVE FVEDSKQGII
RVFLKYGQNN ERVITGLKRI SKPGLRVYAK SNEVPRVLNG LGIAIISTSQ GVLTDKEARA
KQAGGEVLAY VW
