RS8_ECOLI
ID RS8_ECOLI Reviewed; 130 AA.
AC P0A7W7; P02361; Q2M6X1;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=30S ribosomal protein S8;
DE AltName: Full=Small ribosomal subunit protein uS8 {ECO:0000303|PubMed:24524803};
GN Name=rpsH; OrderedLocusNames=b3306, JW3268;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=6222285; DOI=10.1093/nar/11.9.2599;
RA Cerretti D.P., Dean D., Davis G.R., Bedwell D.M., Nomura M.;
RT "The spc ribosomal protein operon of Escherichia coli: sequence and
RT cotranscription of the ribosomal protein genes and a protein export gene.";
RL Nucleic Acids Res. 11:2599-2616(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEIN SEQUENCE OF 2-130, AND SUBUNIT.
RC STRAIN=K;
RX PubMed=365698; DOI=10.1515/bchm2.1978.359.2.1509;
RA Allen G., Wittmann-Liebold B.;
RT "The amino acid sequence of the ribosomal protein S8 of Escherichia coli.";
RL Hoppe-Seyler's Z. Physiol. Chem. 359:1509-1525(1978).
RN [5]
RP MECHANISM OF TRANSLATION REGULATION.
RX PubMed=6262737; DOI=10.1093/nar/9.7.1757;
RA Olins P.O., Nomura M.;
RT "Translational regulation by ribosomal protein S8 in Escherichia coli:
RT structural homology between rRNA binding site and feedback target on
RT mRNA.";
RL Nucleic Acids Res. 9:1757-1764(1981).
RN [6]
RP MUTAGENESIS.
RX PubMed=1735715; DOI=10.1128/jb.174.4.1213-1221.1992;
RA Wower I., Kowaleski M.P., Sears L.E., Zimmermann R.A.;
RT "Mutagenesis of ribosomal protein S8 from Escherichia coli: defects in
RT regulation of the spc operon.";
RL J. Bacteriol. 174:1213-1221(1992).
RN [7]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [8]
RP MASS SPECTROMETRY, AND SUBUNIT.
RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX PubMed=10094780; DOI=10.1006/abio.1998.3077;
RA Arnold R.J., Reilly J.P.;
RT "Observation of Escherichia coli ribosomal proteins and their
RT posttranslational modifications by mass spectrometry.";
RL Anal. Biochem. 269:105-112(1999).
RN [9]
RP NOMENCLATURE.
RX PubMed=24524803; DOI=10.1016/j.sbi.2014.01.002;
RA Ban N., Beckmann R., Cate J.H.D., Dinman J.D., Dragon F., Ellis S.R.,
RA Lafontaine D.L.J., Lindahl L., Liljas A., Lipton J.M., McAlear M.A.,
RA Moore P.B., Noller H.F., Ortega J., Panse V.G., Ramakrishnan V.,
RA Spahn C.M.T., Steitz T.A., Tchorzewski M., Tollervey D., Warren A.J.,
RA Williamson J.R., Wilson D., Yonath A., Yusupov M.;
RT "A new system for naming ribosomal proteins.";
RL Curr. Opin. Struct. Biol. 24:165-169(2014).
RN [10]
RP 3D-STRUCTURE MODELING, AND SUBUNIT.
RX PubMed=12244297; DOI=10.1038/nsb841;
RA Tung C.-S., Joseph S., Sanbonmatsu K.Y.;
RT "All-atom homology model of the Escherichia coli 30S ribosomal subunit.";
RL Nat. Struct. Biol. 9:750-755(2002).
RN [11]
RP STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS), AND SUBUNIT.
RC STRAIN=MRE-600;
RX PubMed=12809609; DOI=10.1016/s0092-8674(03)00427-6;
RA Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M.,
RA Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.;
RT "Study of the structural dynamics of the E. coli 70S ribosome using real-
RT space refinement.";
RL Cell 113:789-801(2003).
RN [12]
RP X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES,
RP AND SUBUNIT.
RC STRAIN=MRE-600;
RX PubMed=16272117; DOI=10.1126/science.1117230;
RA Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A.,
RA Holton J.M., Cate J.H.D.;
RT "Structures of the bacterial ribosome at 3.5 A resolution.";
RL Science 310:827-834(2005).
RN [13]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.0 ANGSTROMS) OF 70S RIBOSOME IN COMPLEX
RP WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=27906160; DOI=10.1038/nature20822;
RA Ma C., Kurita D., Li N., Chen Y., Himeno H., Gao N.;
RT "Mechanistic insights into the alternative translation termination by ArfA
RT and RF2.";
RL Nature 541:550-553(2017).
RN [14]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.1 ANGSTROMS) OF 70S RIBOSOME IN COMPLEX
RP WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=27906161; DOI=10.1038/nature20821;
RA Huter P., Mueller C., Beckert B., Arenz S., Berninghausen O., Beckmann R.,
RA Wilson D.N.;
RT "Structural basis for ArfA-RF2-mediated translation termination on mRNAs
RT lacking stop codons.";
RL Nature 541:546-549(2017).
RN [15]
RP STRUCTURE BY ELECTRON MICROSCOPY (2.97 ANGSTROMS) OF 70S RIBOSOME IN
RP COMPLEX WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=27934701; DOI=10.1126/science.aai9127;
RA James N.R., Brown A., Gordiyenko Y., Ramakrishnan V.;
RT "Translational termination without a stop codon.";
RL Science 354:1437-1440(2016).
RN [16]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.52 ANGSTROMS) OF 70S RIBOSOME IN
RP COMPLEX WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=28077875; DOI=10.1038/nature21053;
RA Zeng F., Chen Y., Remis J., Shekhar M., Phillips J.C., Tajkhorshid E.,
RA Jin H.;
RT "Structural basis of co-translational quality control by ArfA and RF2 bound
RT to ribosome.";
RL Nature 541:554-557(2017).
CC -!- FUNCTION: One of the primary rRNA binding proteins, it binds directly
CC to 16S rRNA central domain where it helps coordinate assembly of the
CC platform of the 30S subunit. {ECO:0000250}.
CC -!- FUNCTION: Protein S8 is a translational repressor protein, it controls
CC the translation of the spc operon by binding to its mRNA.
CC {ECO:0000269|PubMed:6262737}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit (PubMed:365698,
CC PubMed:10094780, PubMed:12809609, PubMed:16272117, PubMed:27934701,
CC PubMed:12244297, PubMed:27906160, PubMed:27906161, PubMed:28077875).
CC Contacts proteins S5 and S12 (By similarity).
CC {ECO:0000250|UniProtKB:Q5SHQ2, ECO:0000269|PubMed:10094780,
CC ECO:0000269|PubMed:12244297, ECO:0000269|PubMed:12809609,
CC ECO:0000269|PubMed:16272117, ECO:0000269|PubMed:27906160,
CC ECO:0000269|PubMed:27906161, ECO:0000269|PubMed:27934701,
CC ECO:0000269|PubMed:28077875, ECO:0000269|PubMed:365698}.
CC -!- MASS SPECTROMETRY: Mass=13993.2; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10094780};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS8 family.
CC {ECO:0000305}.
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DR EMBL; X01563; CAA25719.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58103.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76331.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77985.1; -; Genomic_DNA.
DR PIR; E65123; R3EC8.
DR RefSeq; NP_417765.1; NC_000913.3.
DR RefSeq; WP_000062611.1; NZ_STEB01000038.1.
DR PDB; 1EG0; EM; 11.50 A; E=1-130.
DR PDB; 1S03; X-ray; 2.70 A; G/H=2-130.
DR PDB; 2YKR; EM; 9.80 A; H=2-130.
DR PDB; 3IY8; EM; 14.10 A; H=2-130.
DR PDB; 3J9Y; EM; 3.90 A; h=1-130.
DR PDB; 3J9Z; EM; 3.60 A; SH=2-130.
DR PDB; 3JA1; EM; 3.60 A; SH=2-130.
DR PDB; 3JBU; EM; 3.64 A; H=1-130.
DR PDB; 3JBV; EM; 3.32 A; H=1-130.
DR PDB; 3JCD; EM; 3.70 A; h=1-130.
DR PDB; 3JCE; EM; 3.20 A; h=1-130.
DR PDB; 3JCJ; EM; 3.70 A; p=1-130.
DR PDB; 3JCN; EM; 4.60 A; k=1-130.
DR PDB; 4A2I; EM; 16.50 A; H=2-130.
DR PDB; 4ADV; EM; 13.50 A; H=2-130.
DR PDB; 4U1U; X-ray; 2.95 A; AH/CH=2-130.
DR PDB; 4U1V; X-ray; 3.00 A; AH/CH=2-130.
DR PDB; 4U20; X-ray; 2.90 A; AH/CH=2-130.
DR PDB; 4U24; X-ray; 2.90 A; AH/CH=2-130.
DR PDB; 4U25; X-ray; 2.90 A; AH/CH=2-130.
DR PDB; 4U26; X-ray; 2.80 A; AH/CH=2-130.
DR PDB; 4U27; X-ray; 2.80 A; AH/CH=2-130.
DR PDB; 4V47; EM; 12.30 A; BH=2-130.
DR PDB; 4V48; EM; 11.50 A; BH=2-130.
DR PDB; 4V4H; X-ray; 3.46 A; AH/CH=1-130.
DR PDB; 4V4Q; X-ray; 3.46 A; AH/CH=2-130.
DR PDB; 4V4V; EM; 15.00 A; AH=3-129.
DR PDB; 4V4W; EM; 15.00 A; AH=3-129.
DR PDB; 4V50; X-ray; 3.22 A; AH/CH=2-130.
DR PDB; 4V52; X-ray; 3.21 A; AH/CH=2-130.
DR PDB; 4V53; X-ray; 3.54 A; AH/CH=2-130.
DR PDB; 4V54; X-ray; 3.30 A; AH/CH=2-130.
DR PDB; 4V55; X-ray; 4.00 A; AH/CH=2-130.
DR PDB; 4V56; X-ray; 3.93 A; AH/CH=2-130.
DR PDB; 4V57; X-ray; 3.50 A; AH/CH=2-130.
DR PDB; 4V5B; X-ray; 3.74 A; BH/DH=2-130.
DR PDB; 4V5H; EM; 5.80 A; AH=2-130.
DR PDB; 4V5Y; X-ray; 4.45 A; AH/CH=2-130.
DR PDB; 4V64; X-ray; 3.50 A; AH/CH=2-130.
DR PDB; 4V65; EM; 9.00 A; AV=1-130.
DR PDB; 4V66; EM; 9.00 A; AV=1-130.
DR PDB; 4V69; EM; 6.70 A; AH=2-130.
DR PDB; 4V6C; X-ray; 3.19 A; AH/CH=1-130.
DR PDB; 4V6D; X-ray; 3.81 A; AH/CH=1-130.
DR PDB; 4V6E; X-ray; 3.71 A; AH/CH=1-130.
DR PDB; 4V6K; EM; 8.25 A; BL=1-130.
DR PDB; 4V6L; EM; 13.20 A; AL=1-130.
DR PDB; 4V6M; EM; 7.10 A; AH=2-130.
DR PDB; 4V6N; EM; 12.10 A; BK=2-130.
DR PDB; 4V6O; EM; 14.70 A; AK=2-130.
DR PDB; 4V6P; EM; 13.50 A; AK=2-130.
DR PDB; 4V6Q; EM; 11.50 A; AK=2-130.
DR PDB; 4V6R; EM; 11.50 A; AK=2-130.
DR PDB; 4V6S; EM; 13.10 A; BJ=2-130.
DR PDB; 4V6T; EM; 8.30 A; AH=2-130.
DR PDB; 4V6V; EM; 9.80 A; AH=2-130.
DR PDB; 4V6Y; EM; 12.00 A; AH=1-130.
DR PDB; 4V6Z; EM; 12.00 A; AH=1-130.
DR PDB; 4V70; EM; 17.00 A; AH=1-130.
DR PDB; 4V71; EM; 20.00 A; AH=1-130.
DR PDB; 4V72; EM; 13.00 A; AH=1-130.
DR PDB; 4V73; EM; 15.00 A; AH=1-130.
DR PDB; 4V74; EM; 17.00 A; AH=1-130.
DR PDB; 4V75; EM; 12.00 A; AH=1-130.
DR PDB; 4V76; EM; 17.00 A; AH=1-130.
DR PDB; 4V77; EM; 17.00 A; AH=1-130.
DR PDB; 4V78; EM; 20.00 A; AH=1-130.
DR PDB; 4V79; EM; 15.00 A; AH=1-130.
DR PDB; 4V7A; EM; 9.00 A; AH=1-130.
DR PDB; 4V7B; EM; 6.80 A; AH=1-130.
DR PDB; 4V7C; EM; 7.60 A; AH=2-130.
DR PDB; 4V7D; EM; 7.60 A; BH=2-130.
DR PDB; 4V7I; EM; 9.60 A; BH=1-130.
DR PDB; 4V7S; X-ray; 3.25 A; AH/CH=2-130.
DR PDB; 4V7T; X-ray; 3.19 A; AH/CH=2-130.
DR PDB; 4V7U; X-ray; 3.10 A; AH/CH=2-130.
DR PDB; 4V7V; X-ray; 3.29 A; AH/CH=2-130.
DR PDB; 4V85; X-ray; 3.20 A; AH=1-130.
DR PDB; 4V89; X-ray; 3.70 A; AH=1-130.
DR PDB; 4V9C; X-ray; 3.30 A; AH/CH=1-130.
DR PDB; 4V9D; X-ray; 3.00 A; AH/BH=2-130.
DR PDB; 4V9O; X-ray; 2.90 A; BH/DH/FH/HH=1-130.
DR PDB; 4V9P; X-ray; 2.90 A; BH/DH/FH/HH=1-130.
DR PDB; 4WF1; X-ray; 3.09 A; AH/CH=2-130.
DR PDB; 4WOI; X-ray; 3.00 A; AH/DH=1-130.
DR PDB; 4WWW; X-ray; 3.10 A; QH/XH=2-130.
DR PDB; 4YBB; X-ray; 2.10 A; AH/BH=2-130.
DR PDB; 5AFI; EM; 2.90 A; h=1-130.
DR PDB; 5H5U; EM; 3.00 A; o=2-130.
DR PDB; 5IQR; EM; 3.00 A; m=1-130.
DR PDB; 5IT8; X-ray; 3.12 A; AH/BH=2-130.
DR PDB; 5J5B; X-ray; 2.80 A; AH/BH=2-130.
DR PDB; 5J7L; X-ray; 3.00 A; AH/BH=2-130.
DR PDB; 5J88; X-ray; 3.32 A; AH/BH=2-130.
DR PDB; 5J8A; X-ray; 3.10 A; AH/BH=2-130.
DR PDB; 5J91; X-ray; 2.96 A; AH/BH=2-130.
DR PDB; 5JC9; X-ray; 3.03 A; AH/BH=2-130.
DR PDB; 5JTE; EM; 3.60 A; AH=1-130.
DR PDB; 5JU8; EM; 3.60 A; AH=1-130.
DR PDB; 5KCR; EM; 3.60 A; 1h=1-130.
DR PDB; 5KCS; EM; 3.90 A; 1h=1-130.
DR PDB; 5KPS; EM; 3.90 A; 13=1-130.
DR PDB; 5KPV; EM; 4.10 A; 12=1-130.
DR PDB; 5KPW; EM; 3.90 A; 12=1-130.
DR PDB; 5KPX; EM; 3.90 A; 12=1-130.
DR PDB; 5L3P; EM; 3.70 A; h=1-130.
DR PDB; 5LZA; EM; 3.60 A; h=2-130.
DR PDB; 5LZB; EM; 5.30 A; h=2-130.
DR PDB; 5LZC; EM; 4.80 A; h=2-130.
DR PDB; 5LZD; EM; 3.40 A; h=2-130.
DR PDB; 5LZE; EM; 3.50 A; h=2-130.
DR PDB; 5LZF; EM; 4.60 A; h=2-130.
DR PDB; 5MDV; EM; 2.97 A; m=1-130.
DR PDB; 5MDW; EM; 3.06 A; m=1-130.
DR PDB; 5MDY; EM; 3.35 A; m=1-130.
DR PDB; 5MDZ; EM; 3.10 A; m=1-130.
DR PDB; 5ME0; EM; 13.50 A; H=1-130.
DR PDB; 5ME1; EM; 13.50 A; H=1-130.
DR PDB; 5MGP; EM; 3.10 A; h=2-130.
DR PDB; 5MY1; EM; 7.60 A; H=2-130.
DR PDB; 5NO2; EM; 5.16 A; H=2-130.
DR PDB; 5NO3; EM; 5.16 A; H=2-130.
DR PDB; 5NO4; EM; 5.16 A; H=2-130.
DR PDB; 5NP6; EM; 3.60 A; K=2-130.
DR PDB; 5NWY; EM; 2.93 A; 7=1-130.
DR PDB; 5O2R; EM; 3.40 A; h=2-130.
DR PDB; 5U4I; EM; 3.50 A; h=2-130.
DR PDB; 5U9F; EM; 3.20 A; H=1-130.
DR PDB; 5U9G; EM; 3.20 A; H=1-130.
DR PDB; 5UYK; EM; 3.90 A; H=2-130.
DR PDB; 5UYL; EM; 3.60 A; H=2-130.
DR PDB; 5UYM; EM; 3.20 A; H=2-130.
DR PDB; 5UYN; EM; 4.00 A; H=2-130.
DR PDB; 5UYP; EM; 3.90 A; H=2-130.
DR PDB; 5UYQ; EM; 3.80 A; H=2-130.
DR PDB; 5WDT; EM; 3.00 A; h=2-130.
DR PDB; 5WE4; EM; 3.10 A; h=2-130.
DR PDB; 5WE6; EM; 3.40 A; h=2-130.
DR PDB; 5WFK; EM; 3.40 A; h=2-130.
DR PDB; 6BU8; EM; 3.50 A; H=2-130.
DR PDB; 6BY1; X-ray; 3.94 A; AH/BH=2-130.
DR PDB; 6C4I; EM; 3.24 A; h=1-130.
DR PDB; 6ENF; EM; 3.20 A; h=2-130.
DR PDB; 6ENJ; EM; 3.70 A; h=2-130.
DR PDB; 6ENU; EM; 3.10 A; h=2-130.
DR PDB; 6GWT; EM; 3.80 A; h=2-130.
DR PDB; 6GXM; EM; 3.80 A; h=2-130.
DR PDB; 6GXN; EM; 3.90 A; h=2-130.
DR PDB; 6GXO; EM; 3.90 A; h=2-130.
DR PDB; 6GXP; EM; 4.40 A; h=2-130.
DR PDB; 6H4N; EM; 3.00 A; h=2-130.
DR PDB; 6H58; EM; 7.90 A; h/hh=2-130.
DR PDB; 6HRM; EM; 2.96 A; m=2-130.
DR PDB; 6I7V; X-ray; 2.90 A; AH/BH=2-130.
DR PDB; 6O9J; EM; 3.90 A; h=2-130.
DR PDB; 6O9K; EM; 4.00 A; h=2-130.
DR PDB; 6OFX; EM; 3.30 A; M=2-130.
DR PDB; 6OG7; EM; 3.30 A; M=2-130.
DR PDB; 6ORE; EM; 2.90 A; m=2-130.
DR PDB; 6ORL; EM; 3.50 A; m=2-130.
DR PDB; 6OST; EM; 4.20 A; m=2-130.
DR PDB; 6OT3; EM; 3.90 A; m=2-130.
DR PDB; 6OUO; EM; 3.70 A; m=2-130.
DR PDB; 6Q97; EM; 3.90 A; m=2-130.
DR PDB; 6Q98; EM; 4.30 A; m=1-130.
DR PDB; 6Q9A; EM; 3.70 A; m=2-130.
DR PDB; 6SZS; EM; 3.06 A; h=1-130.
DR PDB; 6TBV; EM; 2.70 A; S081=1-130.
DR PDB; 6TC3; EM; 2.70 A; S081=1-130.
DR PDB; 6VWL; EM; 3.10 A; g=1-130.
DR PDB; 6VWM; EM; 3.40 A; g=1-130.
DR PDB; 6VWN; EM; 3.40 A; g=1-130.
DR PDB; 6W6K; EM; 3.60 A; H=1-130.
DR PDB; 6W77; EM; 3.60 A; H=1-130.
DR PDB; 6W7M; EM; 3.80 A; H=1-130.
DR PDB; 6W7N; EM; 3.40 A; H=1-130.
DR PDB; 6W7W; EM; 3.90 A; G=1-130.
DR PDB; 6WD6; EM; 3.70 A; M=2-130.
DR PDB; 6WDB; EM; 4.00 A; M=2-130.
DR PDB; 6WDC; EM; 4.20 A; M=2-130.
DR PDB; 6WDD; EM; 3.20 A; M=2-130.
DR PDB; 6WDE; EM; 3.00 A; M=2-130.
DR PDB; 6WDF; EM; 3.30 A; M=2-130.
DR PDB; 6WDG; EM; 3.30 A; M=2-130.
DR PDB; 6WDH; EM; 4.30 A; M=2-130.
DR PDB; 6WDI; EM; 4.00 A; M=2-130.
DR PDB; 6WDJ; EM; 3.70 A; M=2-130.
DR PDB; 6WDK; EM; 3.60 A; M=2-130.
DR PDB; 6WDL; EM; 3.70 A; M=2-130.
DR PDB; 6WDM; EM; 3.60 A; M=2-130.
DR PDB; 6WNV; EM; 3.50 A; M=2-130.
DR PDB; 6WNW; EM; 3.20 A; M=2-130.
DR PDB; 6XE0; EM; 6.80 A; G=2-130.
DR PDB; 6XZA; EM; 2.66 A; H1=2-130.
DR PDB; 6XZB; EM; 2.54 A; H1=2-130.
DR PDB; 6Y69; EM; 2.86 A; h=2-130.
DR PDB; 6ZTL; EM; 3.50 A; AH=1-130.
DR PDB; 7ABZ; EM; 3.21 A; m=2-130.
DR PDB; 7AC7; EM; 3.08 A; m=2-130.
DR PDB; 7ACJ; EM; 3.20 A; m=2-130.
DR PDB; 7ACR; EM; 3.44 A; m=2-130.
DR PDB; 7AFI; EM; 3.53 A; H=1-130.
DR PDB; 7AFL; EM; 4.20 A; H=1-130.
DR PDB; 7AFO; EM; 3.93 A; H=1-130.
DR PDB; 7B5K; EM; 2.90 A; h=2-130.
DR PDB; 7BOD; EM; 2.88 A; H=1-130.
DR PDB; 7BOE; EM; 2.90 A; H=1-130.
DR PDB; 7BOF; EM; 2.92 A; H=1-130.
DR PDB; 7BOG; EM; 2.75 A; H=1-130.
DR PDB; 7BOH; EM; 2.82 A; H=1-130.
DR PDB; 7BOI; EM; 2.98 A; H=1-130.
DR PDB; 7D6Z; EM; 3.40 A; o=1-130.
DR PDB; 7D80; EM; 4.10 A; I=1-130.
DR PDB; 7JSS; EM; 3.70 A; M=2-130.
DR PDB; 7JSW; EM; 3.80 A; M=2-130.
DR PDB; 7JSZ; EM; 3.70 A; M=2-130.
DR PDB; 7JT1; EM; 3.30 A; M=2-130.
DR PDB; 7JT2; EM; 3.50 A; M=2-130.
DR PDB; 7JT3; EM; 3.70 A; M=2-130.
DR PDB; 7K50; EM; 3.40 A; M=2-130.
DR PDB; 7K51; EM; 3.50 A; M=2-130.
DR PDB; 7K52; EM; 3.40 A; M=2-130.
DR PDB; 7K53; EM; 3.20 A; M=2-130.
DR PDB; 7K54; EM; 3.20 A; M=2-130.
DR PDB; 7K55; EM; 3.30 A; M=2-130.
DR PDB; 7LV0; EM; 3.20 A; M=2-130.
DR PDB; 7N1P; EM; 2.33 A; SH=1-130.
DR PDB; 7N2C; EM; 2.72 A; SH=1-130.
DR PDB; 7N2U; EM; 2.53 A; SH=1-130.
DR PDB; 7N2V; EM; 2.54 A; SH=1-130.
DR PDB; 7N30; EM; 2.66 A; SH=1-130.
DR PDB; 7N31; EM; 2.69 A; SH=1-130.
DR PDB; 7NAR; EM; 3.00 A; H=1-130.
DR PDB; 7NAS; EM; 3.31 A; H=1-130.
DR PDB; 7NAT; EM; 3.59 A; H=1-130.
DR PDB; 7NAU; EM; 3.78 A; H=1-130.
DR PDB; 7NAV; EM; 4.80 A; H=1-130.
DR PDB; 7NBU; EM; 3.11 A; H=2-130.
DR PDB; 7O19; EM; 2.90 A; AH=1-130.
DR PDB; 7O1A; EM; 2.40 A; AH=1-130.
DR PDB; 7O1C; EM; 2.60 A; AH=1-130.
DR PDB; 7O5H; EM; 3.10 A; H=2-130.
DR PDB; 7OE0; EM; 2.69 A; H=2-130.
DR PDB; 7OE1; EM; 3.05 A; H=2-130.
DR PDB; 7OI0; EM; 2.76 A; H=2-130.
DR PDB; 7OIZ; EM; 2.90 A; H=1-130.
DR PDB; 7OJ0; EM; 3.50 A; H=1-130.
DR PDB; 7P3K; EM; 2.90 A; H=1-130.
DR PDB; 7PJV; EM; 3.10 A; h=1-130.
DR PDB; 7PJY; EM; 3.10 A; h=1-130.
DR PDB; 7QG8; EM; 3.97 A; 7=1-130.
DR PDB; 7QGH; EM; 4.48 A; 7=1-130.
DR PDB; 7SS9; EM; 3.90 A; M=2-130.
DR PDB; 7SSD; EM; 3.30 A; M=2-130.
DR PDB; 7SSL; EM; 3.80 A; M=2-130.
DR PDB; 7SSN; EM; 3.20 A; M=2-130.
DR PDB; 7SSO; EM; 3.20 A; M=2-130.
DR PDB; 7SSW; EM; 3.80 A; M=2-130.
DR PDB; 7ST2; EM; 2.90 A; M=2-130.
DR PDB; 7ST6; EM; 3.00 A; M=2-130.
DR PDB; 7ST7; EM; 3.20 A; M=2-130.
DR PDBsum; 1EG0; -.
DR PDBsum; 1S03; -.
DR PDBsum; 2YKR; -.
DR PDBsum; 3IY8; -.
DR PDBsum; 3J9Y; -.
DR PDBsum; 3J9Z; -.
DR PDBsum; 3JA1; -.
DR PDBsum; 3JBU; -.
DR PDBsum; 3JBV; -.
DR PDBsum; 3JCD; -.
DR PDBsum; 3JCE; -.
DR PDBsum; 3JCJ; -.
DR PDBsum; 3JCN; -.
DR PDBsum; 4A2I; -.
DR PDBsum; 4ADV; -.
DR PDBsum; 4U1U; -.
DR PDBsum; 4U1V; -.
DR PDBsum; 4U20; -.
DR PDBsum; 4U24; -.
DR PDBsum; 4U25; -.
DR PDBsum; 4U26; -.
DR PDBsum; 4U27; -.
DR PDBsum; 4V47; -.
DR PDBsum; 4V48; -.
DR PDBsum; 4V4H; -.
DR PDBsum; 4V4Q; -.
DR PDBsum; 4V4V; -.
DR PDBsum; 4V4W; -.
DR PDBsum; 4V50; -.
DR PDBsum; 4V52; -.
DR PDBsum; 4V53; -.
DR PDBsum; 4V54; -.
DR PDBsum; 4V55; -.
DR PDBsum; 4V56; -.
DR PDBsum; 4V57; -.
DR PDBsum; 4V5B; -.
DR PDBsum; 4V5H; -.
DR PDBsum; 4V5Y; -.
DR PDBsum; 4V64; -.
DR PDBsum; 4V65; -.
DR PDBsum; 4V66; -.
DR PDBsum; 4V69; -.
DR PDBsum; 4V6C; -.
DR PDBsum; 4V6D; -.
DR PDBsum; 4V6E; -.
DR PDBsum; 4V6K; -.
DR PDBsum; 4V6L; -.
DR PDBsum; 4V6M; -.
DR PDBsum; 4V6N; -.
DR PDBsum; 4V6O; -.
DR PDBsum; 4V6P; -.
DR PDBsum; 4V6Q; -.
DR PDBsum; 4V6R; -.
DR PDBsum; 4V6S; -.
DR PDBsum; 4V6T; -.
DR PDBsum; 4V6V; -.
DR PDBsum; 4V6Y; -.
DR PDBsum; 4V6Z; -.
DR PDBsum; 4V70; -.
DR PDBsum; 4V71; -.
DR PDBsum; 4V72; -.
DR PDBsum; 4V73; -.
DR PDBsum; 4V74; -.
DR PDBsum; 4V75; -.
DR PDBsum; 4V76; -.
DR PDBsum; 4V77; -.
DR PDBsum; 4V78; -.
DR PDBsum; 4V79; -.
DR PDBsum; 4V7A; -.
DR PDBsum; 4V7B; -.
DR PDBsum; 4V7C; -.
DR PDBsum; 4V7D; -.
DR PDBsum; 4V7I; -.
DR PDBsum; 4V7S; -.
DR PDBsum; 4V7T; -.
DR PDBsum; 4V7U; -.
DR PDBsum; 4V7V; -.
DR PDBsum; 4V85; -.
DR PDBsum; 4V89; -.
DR PDBsum; 4V9C; -.
DR PDBsum; 4V9D; -.
DR PDBsum; 4V9O; -.
DR PDBsum; 4V9P; -.
DR PDBsum; 4WF1; -.
DR PDBsum; 4WOI; -.
DR PDBsum; 4WWW; -.
DR PDBsum; 4YBB; -.
DR PDBsum; 5AFI; -.
DR PDBsum; 5H5U; -.
DR PDBsum; 5IQR; -.
DR PDBsum; 5IT8; -.
DR PDBsum; 5J5B; -.
DR PDBsum; 5J7L; -.
DR PDBsum; 5J88; -.
DR PDBsum; 5J8A; -.
DR PDBsum; 5J91; -.
DR PDBsum; 5JC9; -.
DR PDBsum; 5JTE; -.
DR PDBsum; 5JU8; -.
DR PDBsum; 5KCR; -.
DR PDBsum; 5KCS; -.
DR PDBsum; 5KPS; -.
DR PDBsum; 5KPV; -.
DR PDBsum; 5KPW; -.
DR PDBsum; 5KPX; -.
DR PDBsum; 5L3P; -.
DR PDBsum; 5LZA; -.
DR PDBsum; 5LZB; -.
DR PDBsum; 5LZC; -.
DR PDBsum; 5LZD; -.
DR PDBsum; 5LZE; -.
DR PDBsum; 5LZF; -.
DR PDBsum; 5MDV; -.
DR PDBsum; 5MDW; -.
DR PDBsum; 5MDY; -.
DR PDBsum; 5MDZ; -.
DR PDBsum; 5ME0; -.
DR PDBsum; 5ME1; -.
DR PDBsum; 5MGP; -.
DR PDBsum; 5MY1; -.
DR PDBsum; 5NO2; -.
DR PDBsum; 5NO3; -.
DR PDBsum; 5NO4; -.
DR PDBsum; 5NP6; -.
DR PDBsum; 5NWY; -.
DR PDBsum; 5O2R; -.
DR PDBsum; 5U4I; -.
DR PDBsum; 5U9F; -.
DR PDBsum; 5U9G; -.
DR PDBsum; 5UYK; -.
DR PDBsum; 5UYL; -.
DR PDBsum; 5UYM; -.
DR PDBsum; 5UYN; -.
DR PDBsum; 5UYP; -.
DR PDBsum; 5UYQ; -.
DR PDBsum; 5WDT; -.
DR PDBsum; 5WE4; -.
DR PDBsum; 5WE6; -.
DR PDBsum; 5WFK; -.
DR PDBsum; 6BU8; -.
DR PDBsum; 6BY1; -.
DR PDBsum; 6C4I; -.
DR PDBsum; 6ENF; -.
DR PDBsum; 6ENJ; -.
DR PDBsum; 6ENU; -.
DR PDBsum; 6GWT; -.
DR PDBsum; 6GXM; -.
DR PDBsum; 6GXN; -.
DR PDBsum; 6GXO; -.
DR PDBsum; 6GXP; -.
DR PDBsum; 6H4N; -.
DR PDBsum; 6H58; -.
DR PDBsum; 6HRM; -.
DR PDBsum; 6I7V; -.
DR PDBsum; 6O9J; -.
DR PDBsum; 6O9K; -.
DR PDBsum; 6OFX; -.
DR PDBsum; 6OG7; -.
DR PDBsum; 6ORE; -.
DR PDBsum; 6ORL; -.
DR PDBsum; 6OST; -.
DR PDBsum; 6OT3; -.
DR PDBsum; 6OUO; -.
DR PDBsum; 6Q97; -.
DR PDBsum; 6Q98; -.
DR PDBsum; 6Q9A; -.
DR PDBsum; 6SZS; -.
DR PDBsum; 6TBV; -.
DR PDBsum; 6TC3; -.
DR PDBsum; 6VWL; -.
DR PDBsum; 6VWM; -.
DR PDBsum; 6VWN; -.
DR PDBsum; 6W6K; -.
DR PDBsum; 6W77; -.
DR PDBsum; 6W7M; -.
DR PDBsum; 6W7N; -.
DR PDBsum; 6W7W; -.
DR PDBsum; 6WD6; -.
DR PDBsum; 6WDB; -.
DR PDBsum; 6WDC; -.
DR PDBsum; 6WDD; -.
DR PDBsum; 6WDE; -.
DR PDBsum; 6WDF; -.
DR PDBsum; 6WDG; -.
DR PDBsum; 6WDH; -.
DR PDBsum; 6WDI; -.
DR PDBsum; 6WDJ; -.
DR PDBsum; 6WDK; -.
DR PDBsum; 6WDL; -.
DR PDBsum; 6WDM; -.
DR PDBsum; 6WNV; -.
DR PDBsum; 6WNW; -.
DR PDBsum; 6XE0; -.
DR PDBsum; 6XZA; -.
DR PDBsum; 6XZB; -.
DR PDBsum; 6Y69; -.
DR PDBsum; 6ZTL; -.
DR PDBsum; 7ABZ; -.
DR PDBsum; 7AC7; -.
DR PDBsum; 7ACJ; -.
DR PDBsum; 7ACR; -.
DR PDBsum; 7AFI; -.
DR PDBsum; 7AFL; -.
DR PDBsum; 7AFO; -.
DR PDBsum; 7B5K; -.
DR PDBsum; 7BOD; -.
DR PDBsum; 7BOE; -.
DR PDBsum; 7BOF; -.
DR PDBsum; 7BOG; -.
DR PDBsum; 7BOH; -.
DR PDBsum; 7BOI; -.
DR PDBsum; 7D6Z; -.
DR PDBsum; 7D80; -.
DR PDBsum; 7JSS; -.
DR PDBsum; 7JSW; -.
DR PDBsum; 7JSZ; -.
DR PDBsum; 7JT1; -.
DR PDBsum; 7JT2; -.
DR PDBsum; 7JT3; -.
DR PDBsum; 7K50; -.
DR PDBsum; 7K51; -.
DR PDBsum; 7K52; -.
DR PDBsum; 7K53; -.
DR PDBsum; 7K54; -.
DR PDBsum; 7K55; -.
DR PDBsum; 7LV0; -.
DR PDBsum; 7N1P; -.
DR PDBsum; 7N2C; -.
DR PDBsum; 7N2U; -.
DR PDBsum; 7N2V; -.
DR PDBsum; 7N30; -.
DR PDBsum; 7N31; -.
DR PDBsum; 7NAR; -.
DR PDBsum; 7NAS; -.
DR PDBsum; 7NAT; -.
DR PDBsum; 7NAU; -.
DR PDBsum; 7NAV; -.
DR PDBsum; 7NBU; -.
DR PDBsum; 7O19; -.
DR PDBsum; 7O1A; -.
DR PDBsum; 7O1C; -.
DR PDBsum; 7O5H; -.
DR PDBsum; 7OE0; -.
DR PDBsum; 7OE1; -.
DR PDBsum; 7OI0; -.
DR PDBsum; 7OIZ; -.
DR PDBsum; 7OJ0; -.
DR PDBsum; 7P3K; -.
DR PDBsum; 7PJV; -.
DR PDBsum; 7PJY; -.
DR PDBsum; 7QG8; -.
DR PDBsum; 7QGH; -.
DR PDBsum; 7SS9; -.
DR PDBsum; 7SSD; -.
DR PDBsum; 7SSL; -.
DR PDBsum; 7SSN; -.
DR PDBsum; 7SSO; -.
DR PDBsum; 7SSW; -.
DR PDBsum; 7ST2; -.
DR PDBsum; 7ST6; -.
DR PDBsum; 7ST7; -.
DR AlphaFoldDB; P0A7W7; -.
DR SMR; P0A7W7; -.
DR BioGRID; 852114; 17.
DR ComplexPortal; CPX-3802; 30S small ribosomal subunit.
DR DIP; DIP-47901N; -.
DR IntAct; P0A7W7; 58.
DR STRING; 511145.b3306; -.
DR DrugBank; DB09093; Chlortetracycline.
DR DrugBank; DB12455; Omadacycline.
DR DrugBank; DB00759; Tetracycline.
DR MoonProt; P0A7W7; -.
DR jPOST; P0A7W7; -.
DR PaxDb; P0A7W7; -.
DR PRIDE; P0A7W7; -.
DR EnsemblBacteria; AAC76331; AAC76331; b3306.
DR EnsemblBacteria; BAE77985; BAE77985; BAE77985.
DR GeneID; 67415347; -.
DR GeneID; 947802; -.
DR KEGG; ecj:JW3268; -.
DR KEGG; eco:b3306; -.
DR PATRIC; fig|1411691.4.peg.3425; -.
DR EchoBASE; EB0900; -.
DR eggNOG; COG0096; Bacteria.
DR HOGENOM; CLU_098428_0_0_6; -.
DR InParanoid; P0A7W7; -.
DR OMA; FDQGYIL; -.
DR PhylomeDB; P0A7W7; -.
DR BioCyc; EcoCyc:EG10907-MON; -.
DR BioCyc; MetaCyc:EG10907-MON; -.
DR EvolutionaryTrace; P0A7W7; -.
DR PRO; PR:P0A7W7; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IDA:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:CAFA.
DR GO; GO:0019843; F:rRNA binding; IDA:EcoliWiki.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:CAFA.
DR GO; GO:0002181; P:cytoplasmic translation; IC:ComplexPortal.
DR GO; GO:0043488; P:regulation of mRNA stability; IMP:EcoliWiki.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IDA:CAFA.
DR HAMAP; MF_01302_B; Ribosomal_S8_B; 1.
DR InterPro; IPR000630; Ribosomal_S8.
DR InterPro; IPR035987; Ribosomal_S8_sf.
DR PANTHER; PTHR11758; PTHR11758; 1.
DR Pfam; PF00410; Ribosomal_S8; 1.
DR SUPFAM; SSF56047; SSF56047; 1.
DR PROSITE; PS00053; RIBOSOMAL_S8; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome; Repressor;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding;
KW Translation regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:365698"
FT CHAIN 2..130
FT /note="30S ribosomal protein S8"
FT /id="PRO_0000126405"
FT CONFLICT 91
FT /note="E -> Q (in Ref. 1; CAA25719)"
FT /evidence="ECO:0000305"
FT HELIX 6..19
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 23..28
FT /evidence="ECO:0007829|PDB:7OE0"
FT HELIX 31..42
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 45..48
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 49..52
FT /evidence="ECO:0007829|PDB:7O5H"
FT STRAND 54..56
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 58..62
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 65..71
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 73..77
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 81..83
FT /evidence="ECO:0007829|PDB:7BOG"
FT HELIX 89..91
FT /evidence="ECO:0007829|PDB:7OE0"
FT TURN 96..99
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 102..106
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 109..111
FT /evidence="ECO:0007829|PDB:7OE0"
FT HELIX 113..118
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 123..130
FT /evidence="ECO:0007829|PDB:7OE0"
SQ SEQUENCE 130 AA; 14127 MW; 74641BC5021DA651 CRC64;
MSMQDPIADM LTRIRNGQAA NKAAVTMPSS KLKVAIANVL KEEGFIEDFK VEGDTKPELE
LTLKYFQGKA VVESIQRVSR PGLRIYKRKD ELPKVMAGLG IAVVSTSKGV MTDRAARQAG
LGGEIICYVA
