BBD1_ARATH
ID BBD1_ARATH Reviewed; 325 AA.
AC Q9FWS6;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Bifunctional nuclease 1;
DE Short=AtBBD1;
DE EC=3.1.-.-;
GN Name=BBD1; OrderedLocusNames=At1g75380; ORFNames=F1B16.9, F1B16_15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20018603; DOI=10.1104/pp.109.147645;
RA You M.K., Shin H.Y., Kim Y.J., Ok S.H., Cho S.K., Jeung J.U., Yoo S.D.,
RA Kim J.K., Shin J.S.;
RT "Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in abscisic
RT acid-mediated callose deposition in Arabidopsis.";
RL Plant Physiol. 152:1015-1029(2010).
CC -!- FUNCTION: Bifunctional nuclease with both RNase and DNase activities.
CC Involved in basal defense response. Participates in abscisic acid-
CC derived callose deposition following infection by a necrotrophic
CC pathogen. {ECO:0000269|PubMed:20018603}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- INDUCTION: Up-regulated by pathogens, abscisic acid, etephon, salicylic
CC acid and methyl jasmonate. {ECO:0000269|PubMed:20018603}.
CC -!- DISRUPTION PHENOTYPE: Increased susceptibility to fungal pathogens.
CC {ECO:0000269|PubMed:20018603}.
CC -!- SIMILARITY: Belongs to the bifunctional nuclease family. {ECO:0000305}.
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DR EMBL; AC023754; AAG13071.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35709.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35710.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35711.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60421.1; -; Genomic_DNA.
DR EMBL; AY054232; AAL06892.1; -; mRNA.
DR EMBL; AY066045; AAL47412.1; -; mRNA.
DR EMBL; AY087417; AAM64965.1; -; mRNA.
DR PIR; C96784; C96784.
DR RefSeq; NP_001322708.1; NM_001334685.1.
DR RefSeq; NP_177671.1; NM_106192.3.
DR RefSeq; NP_849890.1; NM_179559.3.
DR RefSeq; NP_849891.1; NM_179560.3.
DR AlphaFoldDB; Q9FWS6; -.
DR SMR; Q9FWS6; -.
DR BioGRID; 29093; 1.
DR IntAct; Q9FWS6; 1.
DR STRING; 3702.AT1G75380.1; -.
DR iPTMnet; Q9FWS6; -.
DR PaxDb; Q9FWS6; -.
DR PRIDE; Q9FWS6; -.
DR ProteomicsDB; 240819; -.
DR EnsemblPlants; AT1G75380.1; AT1G75380.1; AT1G75380.
DR EnsemblPlants; AT1G75380.2; AT1G75380.2; AT1G75380.
DR EnsemblPlants; AT1G75380.3; AT1G75380.3; AT1G75380.
DR EnsemblPlants; AT1G75380.4; AT1G75380.4; AT1G75380.
DR GeneID; 843874; -.
DR Gramene; AT1G75380.1; AT1G75380.1; AT1G75380.
DR Gramene; AT1G75380.2; AT1G75380.2; AT1G75380.
DR Gramene; AT1G75380.3; AT1G75380.3; AT1G75380.
DR Gramene; AT1G75380.4; AT1G75380.4; AT1G75380.
DR KEGG; ath:AT1G75380; -.
DR Araport; AT1G75380; -.
DR TAIR; locus:2018422; AT1G75380.
DR eggNOG; ENOG502QQ9S; Eukaryota.
DR HOGENOM; CLU_050306_1_0_1; -.
DR InParanoid; Q9FWS6; -.
DR OMA; NFATVCG; -.
DR OrthoDB; 966528at2759; -.
DR PhylomeDB; Q9FWS6; -.
DR PRO; PR:Q9FWS6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FWS6; baseline and differential.
DR Genevisible; Q9FWS6; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0030891; C:VCB complex; IBA:GO_Central.
DR GO; GO:0004518; F:nuclease activity; IDA:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR Gene3D; 3.10.690.10; -; 1.
DR InterPro; IPR036104; BFN_sf.
DR InterPro; IPR003729; Bi_nuclease_dom.
DR InterPro; IPR001943; UVR_dom.
DR Pfam; PF02577; DUF151; 1.
DR Pfam; PF02151; UVR; 1.
DR SUPFAM; SSF103256; SSF103256; 1.
DR PROSITE; PS51658; BFN; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..325
FT /note="Bifunctional nuclease 1"
FT /id="PRO_0000419548"
FT DOMAIN 117..252
FT /note="BFN"
FT DOMAIN 284..318
FT /note="UVR"
SQ SEQUENCE 325 AA; 36174 MW; 6C36D81B3380C71D CRC64;
MRSVQAPVVC PAIRPRQVGA CASLVNYTGL KPRSQFWGNR TKGVKSQGTT TTITLRLCNK
SIKCVFSSHS DGNGSTAENF NENDEEYVNS SVVEAVEVKS GADGFMVKMR DGRQLRCVHN
NPQGGHLPDY APHPAIVLKM EDGTGLLLPI IVLEMPSVLL MAAMTNVQIA RPTMYQVVKE
MVDKMGYEVR LVRVTKRVHE AYFAQLFLSK VGNASECVSF DLRPSDAINI AVRCKIPIQV
NKYLAYSDGM RVIESGKIST PAPASDGLLF TEQDRPNGQA CLDTKEFNIL SKMMQAVDEE
RYDEAAEWRD KLGQFRAKRN LRKYT
