ID   BBD1_ORYSJ              Reviewed;         331 AA.
AC   Q5N8J3; A0A0P0V721;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Bifunctional nuclease 1;
DE            Short=OsBBD1;
DE            EC=3.1.-.-;
GN   Name=BBD1; OrderedLocusNames=Os01g0702000, LOC_Os01g50622;
GN   ORFNames=OsJ_03158, P0047E11.30, P0421H07.6;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=20018603; DOI=10.1104/pp.109.147645;
RA   You M.K., Shin H.Y., Kim Y.J., Ok S.H., Cho S.K., Jeung J.U., Yoo S.D.,
RA   Kim J.K., Shin J.S.;
RT   "Novel bifunctional nucleases, OmBBD and AtBBD1, are involved in abscisic
RT   acid-mediated callose deposition in Arabidopsis.";
RL   Plant Physiol. 152:1015-1029(2010).
CC   -!- FUNCTION: Bifunctional nuclease with both RNase and DNase activities.
CC       Involved in basal defense response. Participates in abscisic acid-
CC       derived callose deposition following infection by a necrotrophic
CC       pathogen. {ECO:0000269|PubMed:20018603}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- INDUCTION: Up-regulated by abscisic acid, etephon, salicylic acid and
CC       methyl jasmonate. {ECO:0000269|PubMed:20018603}.
CC   -!- SIMILARITY: Belongs to the bifunctional nuclease family. {ECO:0000305}.
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DR   EMBL; AP003245; BAD81783.1; -; Genomic_DNA.
DR   EMBL; AP003378; BAD82225.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF05906.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS73900.1; -; Genomic_DNA.
DR   EMBL; CM000138; EEE55256.1; -; Genomic_DNA.
DR   EMBL; AK065414; BAG89510.1; -; mRNA.
DR   RefSeq; XP_015621320.1; XM_015765834.1.
DR   RefSeq; XP_015621322.1; XM_015765836.1.
DR   RefSeq; XP_015621323.1; XM_015765837.1.
DR   RefSeq; XP_015621324.1; XM_015765838.1.
DR   AlphaFoldDB; Q5N8J3; -.
DR   SMR; Q5N8J3; -.
DR   STRING; 4530.OS01T0702000-01; -.
DR   PaxDb; Q5N8J3; -.
DR   PRIDE; Q5N8J3; -.
DR   EnsemblPlants; Os01t0702000-01; Os01t0702000-01; Os01g0702000.
DR   GeneID; 4326932; -.
DR   Gramene; Os01t0702000-01; Os01t0702000-01; Os01g0702000.
DR   KEGG; osa:4326932; -.
DR   eggNOG; ENOG502QQ9S; Eukaryota.
DR   HOGENOM; CLU_050306_1_0_1; -.
DR   InParanoid; Q5N8J3; -.
DR   OMA; QHSTSFW; -.
DR   OrthoDB; 966528at2759; -.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000007752; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   Genevisible; Q5N8J3; OS.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0030891; C:VCB complex; IBA:GO_Central.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR   Gene3D; 3.10.690.10; -; 1.
DR   InterPro; IPR036104; BFN_sf.
DR   InterPro; IPR003729; Bi_nuclease_dom.
DR   Pfam; PF02577; DUF151; 1.
DR   SUPFAM; SSF103256; SSF103256; 1.
DR   PROSITE; PS51658; BFN; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Nuclease; Nucleus; Reference proteome.
FT   CHAIN           1..331
FT                   /note="Bifunctional nuclease 1"
FT                   /id="PRO_0000419550"
FT   DOMAIN          126..261
FT                   /note="BFN"
FT   DOMAIN          291..326
FT                   /note="UVR"
SQ   SEQUENCE   331 AA;  37527 MW;  8D4C43FE7FFA9C49 CRC64;
     MEIINGPVLP RYAAPATGAL TSDAKISGQL LRRVHLRRRA CGLQGDHYRA ARRFFGFPSE
     RHARSGWVWP VCCSYGSSSD GDGAAAADYD ASGEEFVNSS VMEAVELRSV SDGFVIKMRD
     GKNLRCVQNN PRVLRLRDSA PHHAIVLKME DGSDLLLPII VMETPSIMLL AALRNIRIPR
     PTIYNVVKEM TERMGYAVRL VRITEMVHDA YYSRLYLAKI GNEEETISLD LKPSDAINIA
     FRCKVPIQVN RRIAYNNGLK VVQPTPSESY VSSDQFQYTR LDRPDDQPCF EAQEFDLVRN
     MLVAAVEERY KDAAQYRDQL FMFRAKKKNM I