ID   BBKI_BAUBA              Reviewed;         164 AA.
AC   P83052;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Kunitz-type serine protease inhibitor BbKI;
OS   Bauhinia bauhinioides (Perlebia bauhinoides).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Cercidoideae; Cercideae; Bauhiniinae;
OC   Bauhinia.
OX   NCBI_TaxID=166014 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND SUBUNIT.
RC   TISSUE=Seed;
RX   PubMed=11375765; DOI=10.2174/0929867013372779;
RA   Oliva M.L.V., Mendes C.R., Santomauro-Vaz E.M., Juliano M.A., Mentele R.,
RA   Auerswald E.A., Sampaio M.U., Sampaio C.A.M.;
RT   "Bauhinia bauhinioides plasma kallikrein inhibitor: interaction with
RT   synthetic peptides and fluorogenic peptide substrates related to the
RT   reactive site sequence.";
RL   Curr. Med. Chem. 8:977-984(2001).
CC   -!- FUNCTION: Inhibits bovine trypsin, human plasma kallikrein and plasmin
CC       and weakly bovine chymotrypsin. {ECO:0000269|PubMed:11375765}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Temperature dependence:
CC         Thermolabile.;
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:11375765}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I3 (leguminous Kunitz-
CC       type inhibitor) family. {ECO:0000305}.
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DR   PDB; 4ZOT; X-ray; 1.40 A; A=1-164.
DR   PDB; 7JOD; X-ray; 1.33 A; I=1-164.
DR   PDB; 7JOE; X-ray; 2.60 A; I=1-164.
DR   PDB; 7JOS; X-ray; 2.10 A; B/D/F/H/J/L/N=1-164.
DR   PDB; 7JOW; X-ray; 1.91 A; I=1-162.
DR   PDB; 7JQK; X-ray; 1.33 A; I=1-164.
DR   PDB; 7JQN; X-ray; 1.50 A; I=1-164.
DR   PDB; 7JQO; X-ray; 1.60 A; I=1-164.
DR   PDB; 7JQV; X-ray; 2.10 A; I=1-164.
DR   PDB; 7JR1; X-ray; 2.05 A; G/H/I/J/K/L=1-162.
DR   PDB; 7JR2; X-ray; 1.85 A; G/H/I/J/K/L=1-162.
DR   PDB; 7JRX; X-ray; 1.77 A; I/i=1-164.
DR   PDBsum; 4ZOT; -.
DR   PDBsum; 7JOD; -.
DR   PDBsum; 7JOE; -.
DR   PDBsum; 7JOS; -.
DR   PDBsum; 7JOW; -.
DR   PDBsum; 7JQK; -.
DR   PDBsum; 7JQN; -.
DR   PDBsum; 7JQO; -.
DR   PDBsum; 7JQV; -.
DR   PDBsum; 7JR1; -.
DR   PDBsum; 7JR2; -.
DR   PDBsum; 7JRX; -.
DR   AlphaFoldDB; P83052; -.
DR   SMR; P83052; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR011065; Kunitz_inhibitor_STI-like_sf.
DR   InterPro; IPR002160; Prot_inh_Kunz-lg.
DR   PANTHER; PTHR33107; PTHR33107; 1.
DR   Pfam; PF00197; Kunitz_legume; 1.
DR   SMART; SM00452; STI; 1.
DR   SUPFAM; SSF50386; SSF50386; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..164
FT                   /note="Kunitz-type serine protease inhibitor BbKI"
FT                   /id="PRO_0000083295"
FT   STRAND          12..16
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          18..27
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          29..33
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          35..37
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          43..47
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          50..53
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          56..59
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          61..63
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          73..78
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          86..92
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   TURN            93..95
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          96..103
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          110..116
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          119..124
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          133..140
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          143..148
FT                   /evidence="ECO:0007829|PDB:4ZOT"
FT   STRAND          156..160
FT                   /evidence="ECO:0007829|PDB:4ZOT"
SQ   SEQUENCE   164 AA;  17972 MW;  0EB469D4611DD030 CRC64;
     SVVVDTNGQP VSNGADAYYL VPVSHGHAGL ALAKIGNEAE PRAVVLDPHH RPGLPVRFES
     PLRINIIKES YFLNIKFGPS SSDSGVWDVI QQDPIGLAVK VTDTKSLLGP FKVEKEGEGY
     KIVYYPERGQ TGLDIGLVHR NDKYYLAVKD GEPCVFKIRK ATDE