BBLA_TOBAC
ID BBLA_TOBAC Reviewed; 559 AA.
AC F1T160;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=Berberine bridge enzyme-like A {ECO:0000303|PubMed:21343426};
DE Short=NtBBLa {ECO:0000303|PubMed:21343426};
DE EC=1.1.1.- {ECO:0000250|UniProtKB:O64743};
DE Flags: Precursor;
GN Name=BBLA {ECO:0000303|PubMed:21343426}; Synonyms=BBL2.1 {ECO:0000305};
GN ORFNames=LOC107791775 {ECO:0000312|RefSeq:XP_016469399.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, PATHWAY, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION BY JASMONIC ACID, AND
RP COFACTOR.
RC STRAIN=cv. Petit Havana SR1;
RX PubMed=21343426; DOI=10.1104/pp.110.170878;
RA Kajikawa M., Shoji T., Kato A., Hashimoto T.;
RT "Vacuole-localized berberine bridge enzyme-like proteins are required for a
RT late step of nicotine biosynthesis in tobacco.";
RL Plant Physiol. 155:2010-2022(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90;
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [3]
RP REVIEW ON ALKALOID BIOSYNTHESIS IN NICOTIANA TABACUM.
RX PubMed=23953973; DOI=10.1016/j.phytochem.2013.06.002;
RA Dewey R.E., Xie J.;
RT "Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum.";
RL Phytochemistry 94:10-27(2013).
RN [4]
RP REVIEW ON NICOTINE BIOSYNTHESIS.
RX PubMed=25582664; DOI=10.1007/s00438-015-0989-7;
RA Wang X., Bennetzen J.L.;
RT "Current status and prospects for the study of Nicotiana genomics,
RT genetics, and nicotine biosynthesis genes.";
RL Mol. Genet. Genomics 290:11-21(2015).
CC -!- FUNCTION: Involved in the biosynthesis of pyridine alkaloid natural
CC products, leading mainly to the production of anabasine, anatabine,
CC nicotine and nornicotine, effective deterrents against herbivores with
CC antiparasitic and pesticide properties (neurotoxins); nornicotine
CC serves as the precursor in the synthesis of the carcinogen compound N'-
CC nitrosonornicotine (NNN) (PubMed:21343426). Catalyzes a late oxidation
CC step subsequent to the pyridine ring condensation reaction in the
CC biosynthesis of alkaloids (PubMed:21343426).
CC {ECO:0000269|PubMed:21343426}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000269|PubMed:21343426};
CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis.
CC {ECO:0000269|PubMed:21343426}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:21343426}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots.
CC {ECO:0000269|PubMed:21343426}.
CC -!- INDUCTION: By jasmonic acid (MeJA). {ECO:0000269|PubMed:21343426}.
CC -!- DISRUPTION PHENOTYPE: Strongly reduced nicotine biosynthesis but
CC accumulation of dihydromethanicotine (PubMed:21343426). Inhibition of
CC jasmonate-elicited formation of anatabine and other pyridine alkaloids
CC (PubMed:21343426). {ECO:0000269|PubMed:21343426}.
CC -!- SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; AB604219; BAK18779.1; -; mRNA.
DR RefSeq; NP_001312453.1; NM_001325524.1.
DR RefSeq; XP_016469399.1; XM_016613913.1.
DR SMR; F1T160; -.
DR GeneID; 107791775; -.
DR KEGG; nta:107791775; -.
DR OMA; HVPHNIT; -.
DR OrthoDB; 1049549at2759; -.
DR UniPathway; UPA00107; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0000325; C:plant-type vacuole; IDA:UniProtKB.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0009821; P:alkaloid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0042179; P:nicotine biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IMP:UniProtKB.
DR Gene3D; 3.30.43.10; -; 1.
DR Gene3D; 3.30.465.10; -; 1.
DR InterPro; IPR012951; BBE.
DR InterPro; IPR016166; FAD-bd_PCMH.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016167; FAD-bd_PCMH_sub1.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR006094; Oxid_FAD_bind_N.
DR Pfam; PF08031; BBE; 1.
DR Pfam; PF01565; FAD_binding_4; 1.
DR SUPFAM; SSF56176; SSF56176; 1.
DR PROSITE; PS51387; FAD_PCMH; 1.
PE 2: Evidence at transcript level;
KW Alkaloid metabolism; Disulfide bond; FAD; Flavoprotein; Glycoprotein;
KW Oxidoreductase; Reference proteome; Signal; Vacuole.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..559
FT /note="Berberine bridge enzyme-like A"
FT /id="PRO_5015090528"
FT DOMAIN 64..240
FT /note="FAD-binding PCMH-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00718"
FT MOD_RES 101
FT /note="Pros-8alpha-FAD histidine"
FT /evidence="ECO:0000250|UniProtKB:Q9FI21"
FT CARBOHYD 25
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 37
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 321
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 355
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 494
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 29..86
FT /evidence="ECO:0000250|UniProtKB:O64743"
SQ SEQUENCE 559 AA; 62194 MW; 169841C895053F94 CRC64;
MFPLIILISF SLASLSETAT GAVTNLSACL INHNVHNFSI YPTSRNYFNL LHFSLQNLRF
AAPFMPKPTF IILPSSKEEL VSTIFCCRKA SYEIRVRCGG HSYEGTSYVS FDASPFVIVD
LMKLDDVSVD LDSETAWAQG GATIGQIYYA IAKVSDVHAF SAGSGPTVGS GGHISGGGFG
LLSRKFGLAA DNVVDALLID ADGRLLDRKA MGEDVFWAIR GGGGGNWGIV YAWKIRLLKV
PKIVTTCMIY RPGSKQYVAQ ILEKWQIVTP NLVDDFTLGV LLRPADLPAD MKYGNTTPIE
IFPQFNALYL GPKTEVLSIS NETFPELGVK NDECKEMTWV ESALFFSELA DVNGNSTGDI
SRLKERYMDG KGFFKGKTDY VKKPVSMDGM LTFLVELEKN PKGYLVFDPY GGAMDKISDQ
AIAFPHRKGN LFAIQYLAQW NEEDDYMSDV YMEWIRGFYN TMTPFVSSSP RGAYINYLDM
DLGVNMVDDY LLRNASSSSP SSSVDAVERA RAWGEMYFLH NYDRLVKAKT QIDPLNVFRH
EQSIPPMLGS TQEHKYSSE
