BBLB_TOBAC
ID BBLB_TOBAC Reviewed; 568 AA.
AC A7WPL6;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Berberine bridge enzyme-like B {ECO:0000303|PubMed:21343426};
DE Short=NtBBLb {ECO:0000303|PubMed:21343426};
DE EC=1.1.1.- {ECO:0000250|UniProtKB:O64743};
DE Flags: Precursor;
GN Name=BBLB {ECO:0000303|PubMed:21343426};
GN Synonyms=BBL2.2 {ECO:0000305}, T440 {ECO:0000303|PubMed:12826618,
GN ECO:0000303|PubMed:18001808};
GN ORFNames=LOC107830440 {ECO:0000312|RefSeq:XP_016513471.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION BY JASMONIC ACID.
RC STRAIN=cv. Bright Yellow 2; TISSUE=Protoplast;
RX PubMed=12826618; DOI=10.1073/pnas.1032967100;
RA Goossens A., Haekkinen S.T., Laakso I., Seppaenen-Laakso T., Biondi S.,
RA De Sutter V., Lammertyn F., Nuutila A.M., Soederlund H., Zabeau M.,
RA Inze D., Oksman-Caldentey K.-M.;
RT "A functional genomics approach toward the understanding of secondary
RT metabolism in plant cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:8595-8600(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90;
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [3]
RP REVIEW.
RC TISSUE=Protoplast;
RX PubMed=18001808; DOI=10.1016/j.phytochem.2007.09.010;
RA Haekkinen S.T., Tilleman S., Swiatek A., De Sutter V., Rischer H.,
RA Vanhoutte I., Van Onckelen H., Hilson P., Inze D., Oksman-Caldentey K.-M.,
RA Goossens A.;
RT "Functional characterisation of genes involved in pyridine alkaloid
RT biosynthesis in tobacco.";
RL Phytochemistry 68:2773-2785(2007).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, PATHWAY, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND INDUCTION BY JASMONIC ACID.
RC STRAIN=cv. Petit Havana SR1;
RX PubMed=21343426; DOI=10.1104/pp.110.170878;
RA Kajikawa M., Shoji T., Kato A., Hashimoto T.;
RT "Vacuole-localized berberine bridge enzyme-like proteins are required for a
RT late step of nicotine biosynthesis in tobacco.";
RL Plant Physiol. 155:2010-2022(2011).
RN [5]
RP REVIEW ON ALKALOID BIOSYNTHESIS IN NICOTIANA TABACUM.
RX PubMed=23953973; DOI=10.1016/j.phytochem.2013.06.002;
RA Dewey R.E., Xie J.;
RT "Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum.";
RL Phytochemistry 94:10-27(2013).
RN [6]
RP REVIEW ON NICOTINE BIOSYNTHESIS.
RX PubMed=25582664; DOI=10.1007/s00438-015-0989-7;
RA Wang X., Bennetzen J.L.;
RT "Current status and prospects for the study of Nicotiana genomics,
RT genetics, and nicotine biosynthesis genes.";
RL Mol. Genet. Genomics 290:11-21(2015).
CC -!- FUNCTION: Involved in the biosynthesis of pyridine alkaloid natural
CC products, leading mainly to the production of anabasine, anatabine,
CC nicotine and nornicotine, effective deterrents against herbivores with
CC antiparasitic and pesticide properties (neurotoxins); nornicotine
CC serves as the precursor in the synthesis of the carcinogen compound N'-
CC nitrosonornicotine (NNN) (PubMed:21343426). Catalyzes a late oxidation
CC step subsequent to the pyridine ring condensation reaction in the
CC biosynthesis of alkaloids (PubMed:21343426).
CC {ECO:0000269|PubMed:21343426}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000250|UniProtKB:O64743};
CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis.
CC {ECO:0000269|PubMed:21343426}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:21343426}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots.
CC {ECO:0000269|PubMed:21343426}.
CC -!- INDUCTION: By jasmonic acid (MeJA). {ECO:0000269|PubMed:12826618,
CC ECO:0000269|PubMed:21343426}.
CC -!- DISRUPTION PHENOTYPE: Strongly reduced nicotine biosynthesis but
CC accumulation of dihydromethanicotine (PubMed:21343426). Inhibition of
CC jasmonate-elicited formation of anatabine and other pyridine alkaloids
CC (PubMed:21343426). {ECO:0000269|PubMed:21343426}.
CC -!- SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; AM851017; CAO99130.1; -; mRNA.
DR RefSeq; NP_001313171.1; NM_001326242.1.
DR RefSeq; XP_016513471.1; XM_016657985.1.
DR SMR; A7WPL6; -.
DR GeneID; 107830440; -.
DR KEGG; nta:107830440; -.
DR OMA; GEARMAW; -.
DR OrthoDB; 1049549at2759; -.
DR UniPathway; UPA00107; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0000325; C:plant-type vacuole; IDA:UniProtKB.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0009821; P:alkaloid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0042179; P:nicotine biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR Gene3D; 3.30.43.10; -; 1.
DR Gene3D; 3.30.465.10; -; 1.
DR InterPro; IPR012951; BBE.
DR InterPro; IPR016166; FAD-bd_PCMH.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016167; FAD-bd_PCMH_sub1.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR006094; Oxid_FAD_bind_N.
DR Pfam; PF08031; BBE; 1.
DR Pfam; PF01565; FAD_binding_4; 1.
DR SUPFAM; SSF56176; SSF56176; 1.
DR PROSITE; PS51387; FAD_PCMH; 1.
PE 2: Evidence at transcript level;
KW Alkaloid metabolism; Disulfide bond; FAD; Flavoprotein; Glycoprotein;
KW Oxidoreductase; Reference proteome; Signal; Vacuole.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..568
FT /note="Berberine bridge enzyme-like B"
FT /id="PRO_5015086581"
FT DOMAIN 73..249
FT /note="FAD-binding PCMH-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00718"
FT MOD_RES 110
FT /note="Pros-8alpha-FAD histidine"
FT /evidence="ECO:0000250|UniProtKB:Q9FI21"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 364
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 503
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 508
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 33..95
FT /evidence="ECO:0000250|UniProtKB:O64743"
SQ SEQUENCE 568 AA; 63159 MW; 08F26F448DBBFD31 CRC64;
MFPLIILISF SLTSLSATAT SGAGGGVANL YTCLIDHNVH NFSIYPTKND QSSSNYFNLL
DFSLQNLRFA ASYMPKPTVI ILPNSKEELV STILCCRQAS YEIRVRCGGH SYEGTSYVSF
DGSPFVIVDL MKLDEVSVDL DSETAWAQGG ATIGQIYYAI AKVSDVHAFS AGSGPTVGSG
GHISGGGFGL LSRKFGLAAD NVVDALLIDA DGRLLDRKAM GEDVFWAIRG GGGGNWGIIY
AWKIRLLKVP KIVTTCMIYR PGSKQYVAQL LQKWQIVTPN LVDDFTLGVL LRPADLPADM
KYGNSTPIEI FPQFNALYLG PKTEVLSISN EEFPELGVKN DECKEMTWIE SALFFSELAD
INGNSSNDIS RLKERYMDGK GFFKGKTDYV KKPVSMDGML TFLVELEKNP KGYLVFDPYG
GAMDKIDDQA IAFPHRKGNL FAIQYLAQWN EEDDYKSDVY MEWIRGFYNT MTPFVSSSPR
GAYINYLDMD LGVNMDDDYL LRNASSRNSS SSVDAVERAR AWGEMYFLHN YDRLVKAKTQ
IDPLNVFRHE QSIPPMLGST QEHKYSSE
