BBLC1_TOBAC
ID BBLC1_TOBAC Reviewed; 562 AA.
AC F1T161; A0A1S4DN73;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Berberine bridge enzyme-like C-1 {ECO:0000303|PubMed:21343426};
DE Short=NtBBLc {ECO:0000303|PubMed:21343426};
DE EC=1.1.1.- {ECO:0000250|UniProtKB:O64743};
DE Flags: Precursor;
GN Name=BBLC {ECO:0000303|PubMed:21343426};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, PATHWAY, TISSUE
RP SPECIFICITY, SUBCELLULAR LOCATION, AND INDUCTION BY JASMONIC ACID.
RC STRAIN=cv. Petit Havana SR1;
RX PubMed=21343426; DOI=10.1104/pp.110.170878;
RA Kajikawa M., Shoji T., Kato A., Hashimoto T.;
RT "Vacuole-localized berberine bridge enzyme-like proteins are required for a
RT late step of nicotine biosynthesis in tobacco.";
RL Plant Physiol. 155:2010-2022(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 168-562.
RC STRAIN=cv. TN90;
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [3]
RP REVIEW ON ALKALOID BIOSYNTHESIS IN NICOTIANA TABACUM.
RX PubMed=23953973; DOI=10.1016/j.phytochem.2013.06.002;
RA Dewey R.E., Xie J.;
RT "Molecular genetics of alkaloid biosynthesis in Nicotiana tabacum.";
RL Phytochemistry 94:10-27(2013).
RN [4]
RP REVIEW ON NICOTINE BIOSYNTHESIS.
RX PubMed=25582664; DOI=10.1007/s00438-015-0989-7;
RA Wang X., Bennetzen J.L.;
RT "Current status and prospects for the study of Nicotiana genomics,
RT genetics, and nicotine biosynthesis genes.";
RL Mol. Genet. Genomics 290:11-21(2015).
CC -!- FUNCTION: Involved in the biosynthesis of pyridine alkaloid natural
CC products, leading mainly to the production of anabasine, anatabine,
CC nicotine and nornicotine, effective deterrents against herbivores with
CC antiparasitic and pesticide properties (neurotoxins); nornicotine
CC serves as the precursor in the synthesis of the carcinogen compound N'-
CC nitrosonornicotine (NNN) (PubMed:21343426). Catalyzes a late oxidation
CC step subsequent to the pyridine ring condensation reaction in the
CC biosynthesis of alkaloids (PubMed:21343426).
CC {ECO:0000269|PubMed:21343426}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000250|UniProtKB:O64743};
CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis.
CC {ECO:0000269|PubMed:21343426}.
CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000269|PubMed:21343426}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in roots.
CC {ECO:0000269|PubMed:21343426}.
CC -!- INDUCTION: By jasmonic acid (MeJA). {ECO:0000269|PubMed:21343426}.
CC -!- DISRUPTION PHENOTYPE: Strongly reduced nicotine biosynthesis but
CC accumulation of dihydromethanicotine (PubMed:21343426). Inhibition of
CC jasmonate-elicited formation of anatabine and other pyridine alkaloids
CC (PubMed:21343426). {ECO:0000269|PubMed:21343426}.
CC -!- SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase
CC family. {ECO:0000305}.
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DR EMBL; AB604220; BAK18780.1; -; mRNA.
DR RefSeq; NP_001313194.1; NM_001326265.1.
DR RefSeq; XP_016514850.1; XM_016659364.1.
DR SMR; F1T161; -.
DR GeneID; 107831580; -.
DR KEGG; nta:107831580; -.
DR OrthoDB; 1049549at2759; -.
DR UniPathway; UPA00107; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0000325; C:plant-type vacuole; IDA:UniProtKB.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0009821; P:alkaloid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0042179; P:nicotine biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR Gene3D; 3.30.43.10; -; 1.
DR Gene3D; 3.30.465.10; -; 1.
DR InterPro; IPR012951; BBE.
DR InterPro; IPR016166; FAD-bd_PCMH.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016167; FAD-bd_PCMH_sub1.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR006094; Oxid_FAD_bind_N.
DR Pfam; PF08031; BBE; 1.
DR Pfam; PF01565; FAD_binding_4; 1.
DR SUPFAM; SSF56176; SSF56176; 1.
DR PROSITE; PS51387; FAD_PCMH; 1.
PE 2: Evidence at transcript level;
KW Alkaloid metabolism; Disulfide bond; FAD; Flavoprotein; Glycoprotein;
KW Oxidoreductase; Reference proteome; Signal; Vacuole.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..562
FT /note="Berberine bridge enzyme-like C-1"
FT /id="PRO_5003270913"
FT DOMAIN 68..244
FT /note="FAD-binding PCMH-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00718"
FT MOD_RES 105
FT /note="Pros-8alpha-FAD histidine"
FT /evidence="ECO:0000250|UniProtKB:Q9FI21"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 41
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 359
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 498
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 558
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 33..90
FT /evidence="ECO:0000250|UniProtKB:O64743"
SQ SEQUENCE 562 AA; 62112 MW; 33B9C1AF90A904A0 CRC64;
MFPLIILISF SFTFLSASAT SGAGEGVANL STCLINHNVH NFSMYPTSRN YFNLLDFSLQ
NLRFAASNMP KPTVIILPNS KEELVSTILC CRQTSYEIRV RCGGHSYEGT SSVSFDGSPF
VIIDLMKLDD VSVDLDSETA WAQGGATIGQ IYYAIAKASD VHAFSAGSGP TVGSGGHISG
GGFGLLSRKF GVAADSVVDA LLIDADGRLL DRKAMGEDVF WAIRGGGGGN WGIIYAWKIR
LVKVPKIVTT FKISKPGSKQ YVAPLLYKWQ IVAPNLADDF TLGVQMIPID LPADMKYGNP
TPIEICPQFN GLYLGPKTEA VSILNEAFPE LNVKNDDAKE MTWIESALFF SDLDNIFGNS
SDDISHLKER YLGVKICFKG KSDYVKTPFS MDGIMTALVE HEKNPNAFLV FDPYGGAMDK
ISAQAIAFPH RKGNLFAIQY YAQWNEEDDA KSNEHIEWIR GFYNKMAPFV SSSPRGAYVN
YLDMDLGMNM DDDYLLRNAS SRYSSSVDAV ERARAWGEKY FLNNYDRLVK AKTKIDPLNV
FRHEQSIPPT LGSTQEHNYS SE
