ID   BBOF1_MACFA             Reviewed;         529 AA.
AC   Q4R7Y8; Q4R8W1;
DT   03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 2.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Basal body-orientation factor 1 {ECO:0000250|UniProtKB:Q8ND07};
DE   AltName: Full=Coiled-coil domain-containing protein 176;
GN   Name=BBOF1 {ECO:0000250|UniProtKB:Q8ND07}; Synonyms=CCDC176;
GN   ORFNames=QtsA-11302;
OS   Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC   Cercopithecidae; Cercopithecinae; Macaca.
OX   NCBI_TaxID=9541;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 202-529 (ISOFORM 1).
RC   TISSUE=Testis;
RG   International consortium for macaque cDNA sequencing and analysis;
RT   "DNA sequences of macaque genes expressed in brain or testis and its
RT   evolutionary implications.";
RL   Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Basal body protein required in multiciliate cells to align
CC       and maintain cilia orientation in response to flow. May act by
CC       mediating a maturation step that stabilizes and aligns cilia
CC       orientation. Not required to respond to planar cell polarity (PCP) or
CC       flow-based orientation cues (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC       {ECO:0000250}. Note=Localizes to a polar structure adjacent to the
CC       basal body. {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q4R7Y8-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q4R7Y8-2; Sequence=VSP_023956, VSP_023957;
CC   -!- SIMILARITY: Belongs to the BBOF1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE00784.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB168336; BAE00460.1; -; mRNA.
DR   EMBL; AB168673; BAE00784.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001270683.1; NM_001283754.1.
DR   AlphaFoldDB; Q4R7Y8; -.
DR   SMR; Q4R7Y8; -.
DR   STRING; 9541.XP_005561809.1; -.
DR   PRIDE; Q4R7Y8; -.
DR   GeneID; 101925467; -.
DR   CTD; 80127; -.
DR   eggNOG; ENOG502QRCW; Eukaryota.
DR   OrthoDB; 792496at2759; -.
DR   Proteomes; UP000233100; Unplaced.
DR   GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0044458; P:motile cilium assembly; ISS:UniProtKB.
DR   InterPro; IPR029582; Bbof1.
DR   InterPro; IPR032777; DUF4515.
DR   PANTHER; PTHR14845:SF5; PTHR14845:SF5; 1.
DR   Pfam; PF14988; DUF4515; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell projection; Cilium; Coiled coil; Cytoplasm;
KW   Cytoskeleton; Reference proteome.
FT   CHAIN           1..529
FT                   /note="Basal body-orientation factor 1"
FT                   /id="PRO_0000252294"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          510..529
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          55..198
FT                   /evidence="ECO:0000255"
FT   COILED          271..361
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         404..430
FT                   /note="PKIRTFDGREHSTNSVNQDLLEAEKWT -> LLENFLREKTKILTQKLPVCH
FT                   RNWCLP (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_023956"
FT   VAR_SEQ         431..529
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_023957"
FT   CONFLICT        245
FT                   /note="G -> A (in Ref. 1; BAE00460)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   529 AA;  61763 MW;  93251F2CE96CC1F4 CRC64;
     MPSKGKDKKK GKSRGKDTKK LIKTDESVVD RAKANASLWE ARLEITELSR IEYRDTSRTL
     AKSNEDLKKK QCKMEKDIMS VLSYLKKQDQ EKDNMIEKLK QQLNETKEKA QEEKDKLEQK
     YTRQIDELEG QFHQKAKEIG MIHTELKAVR QFQRRKIQVE RELDDLKENL RNTERIHQET
     LRRLESRFFE EKHRLKQEAE KKIIMLAERA HHEAVVQLND AGRNVFKENV YLQKALAYHL
     KEAGGLQKNS QKLQESHALL LHQKEINDLL IKEKIMQLVQ QRSQIQTLQK KVVNLETALG
     YMTKEFESEV LKLQQHAMIE NQAGQVEIDK LQHLLQMKDR EMNRVKKLAK NILDERTEVE
     RFFLDALHQV KQQILISRKH YKQIAQAAFN LKMRAACAGR TEYPKIRTFD GREHSTNSVN
     QDLLEAEKWT HIEGNVDIGD LTWEQKEKVL RLLFAKMNGC PSRKYNQSSK PPVPDYVVSD
     SGETKEFGDE SKLQDKIFIT QQIPISDSSG KVVLPTIPKG PQESDTGTF