ID   BBOF1_XENLA             Reviewed;         531 AA.
AC   A0JMY4;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Basal body-orientation factor 1;
DE   AltName: Full=Coiled-coil domain-containing protein 176;
GN   Name=ccdc176; Synonyms=bbof1;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=23900544; DOI=10.1242/dev.096727;
RA   Chien Y.H., Werner M.E., Stubbs J., Joens M.S., Li J., Chien S.,
RA   Fitzpatrick J.A., Mitchell B.J., Kintner C.;
RT   "Bbof1 is required to maintain cilia orientation.";
RL   Development 140:3468-3477(2013).
CC   -!- FUNCTION: Basal body protein required in multiciliate cells to align
CC       and maintain cilia orientation in response to flow. May act by
CC       mediating a maturation step that stabilizes and aligns cilia
CC       orientation. Not required to respond to planar cell polarity (PCP) or
CC       flow-based orientation cues. {ECO:0000269|PubMed:23900544}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium basal body
CC       {ECO:0000269|PubMed:23900544}. Note=Localizes to a polar structure
CC       adjacent to the basal body.
CC   -!- TISSUE SPECIFICITY: Multiciliated cells. {ECO:0000269|PubMed:23900544}.
CC   -!- INDUCTION: Up-regulated by foxj1 during motile cilia formation.
CC       {ECO:0000269|PubMed:23900544}.
CC   -!- DISRUPTION PHENOTYPE: Cilia in multiciliate cells are motile but fail
CC       to produce flow. Multiciliated cells are present in the normal number,
CC       cilia of normal length and of normal density. They are however
CC       misoriented. {ECO:0000269|PubMed:23900544}.
CC   -!- SIMILARITY: Belongs to the BBOF1 family. {ECO:0000305}.
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DR   EMBL; BC126050; AAI26051.1; -; mRNA.
DR   RefSeq; NP_001090552.1; NM_001097083.1.
DR   AlphaFoldDB; A0JMY4; -.
DR   SMR; A0JMY4; -.
DR   PRIDE; A0JMY4; -.
DR   DNASU; 100036788; -.
DR   GeneID; 100036788; -.
DR   KEGG; xla:100036788; -.
DR   CTD; 100036788; -.
DR   Xenbase; XB-GENE-5831975; bbof1.L.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 100036788; Expressed in internal ear and 10 other tissues.
DR   GO; GO:0036064; C:ciliary basal body; IDA:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0044458; P:motile cilium assembly; IMP:UniProtKB.
DR   InterPro; IPR029582; Bbof1.
DR   InterPro; IPR032777; DUF4515.
DR   PANTHER; PTHR14845:SF5; PTHR14845:SF5; 1.
DR   Pfam; PF14988; DUF4515; 1.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Reference proteome.
FT   CHAIN           1..531
FT                   /note="Basal body-orientation factor 1"
FT                   /id="PRO_0000281127"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          465..505
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          26..213
FT                   /evidence="ECO:0000255"
FT   COILED          274..365
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..21
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   531 AA;  62167 MW;  1C419D11BDA0118D CRC64;
     MPKLKVKAGK GKKGKRKKAG KNEHRLDKES EVERARSNAA LWEARLKVTE FSRVEYRDAA
     RSLAQNNEDL TKQQYKLEKD MVEVIGFLKK QDLEKDELIE KLQQNLIAQK KSAQDEREKL
     VELYSKQIAH LEENYSQKTN EMQIIQSEFK LMREFRRQKV ELEKELDEVK ESLWRANQDH
     KETLARMERR FFEEKQRLEK EAEKKIMMLA EKAHSEAIIQ LDEARKSVFK ENVRLKEAFS
     YHLKEMKDIK KSKKMQEDAK LHLLQEKETN DLLVQEKVSQ VSQKKVQIQE LQQNVKALEC
     ALERMTMEME KDAQGKEHQA LLQEQAGNVE LQKLQKVLHM KEREMNRIKK LARNILEERT
     EVESFFLEAL WQVKQEIATS RNYYRQVAQS AYTSKMIQAS LGKDQYPKIR TFHNKEHSTN
     DVSHDLSEAE KWTHIQAGKV DIGDLTWEQK EKVLRLLFAK MNGFQSRKSP GLKPSPPADV
     SSIKEKEINT SNLEEKPEES SSTFITQSIP ELPAPSLVLP HIQTGRCQVT G