RS9_ECOLI
ID RS9_ECOLI Reviewed; 130 AA.
AC P0A7X3; P02363; Q2M8Y3;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=30S ribosomal protein S9;
DE AltName: Full=Small ribosomal subunit protein uS9 {ECO:0000303|PubMed:24524803};
GN Name=rpsI; OrderedLocusNames=b3230, JW3199;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3884974; DOI=10.1007/bf00383007;
RA Isono S., Thamm S., Kitakawa M., Isono K.;
RT "Cloning and nucleotide sequencing of the genes for ribosomal proteins S9
RT (rpsI) and L13 (rplM) of Escherichia coli.";
RL Mol. Gen. Genet. 198:279-282(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PROTEIN SEQUENCE OF 2-130, AND SUBUNIT.
RC STRAIN=K;
RX PubMed=1091515; DOI=10.1016/0014-5793(75)80656-9;
RA Chen R., Wittmann-Liebold B.;
RT "The primary structure of protein S9 from the 30S subunit of Escherichia
RT coli ribosomes.";
RL FEBS Lett. 52:139-140(1975).
RN [5]
RP PROTEIN SEQUENCE OF 121-130, SUBUNIT, AND CROSS-LINKING TO RRNA.
RC STRAIN=MRE-600;
RX PubMed=7556101; DOI=10.1002/j.1460-2075.1995.tb00137.x;
RA Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.;
RT "Protein-rRNA binding features and their structural and functional
RT implications in ribosomes as determined by cross-linking studies.";
RL EMBO J. 14:4578-4588(1995).
RN [6]
RP INVOLVEMENT IN FORMATION OF THE EF-G/RIBOSOME COMPLEX.
RC STRAIN=B/2;
RX PubMed=4346030; DOI=10.1073/pnas.70.1.151;
RA Marsh R.C., Parmeggiani A.;
RT "Requirement of proteins S5 and S9 from 30S subunits for the ribosome-
RT dependent GTPase activity of elongation factor G.";
RL Proc. Natl. Acad. Sci. U.S.A. 70:151-155(1973).
RN [7]
RP CROSS-LINKING TO THE TRNA CENTRAL FOLD.
RC STRAIN=MRE-600;
RX PubMed=8524654; DOI=10.1093/nar/23.22.4635;
RA Osswald M., Doering T., Brimacombe R.;
RT "The ribosomal neighbourhood of the central fold of tRNA: cross-links from
RT position 47 of tRNA located at the A, P or E site.";
RL Nucleic Acids Res. 23:4635-4641(1995).
RN [8]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
RN [9]
RP ROLE IN P SITE TRNA-BINDING, SUBUNIT, AND MUTAGENESIS OF 105-THR--ARG-130
RP AND 128-SER--ARG-130.
RC STRAIN=CSH142;
RX PubMed=15308780; DOI=10.1073/pnas.0405227101;
RA Hoang L., Fredrick K., Noller H.F.;
RT "Creating ribosomes with an all-RNA 30S subunit P site.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:12439-12443(2004).
RN [10]
RP MASS SPECTROMETRY, AND SUBUNIT.
RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX PubMed=10094780; DOI=10.1006/abio.1998.3077;
RA Arnold R.J., Reilly J.P.;
RT "Observation of Escherichia coli ribosomal proteins and their
RT posttranslational modifications by mass spectrometry.";
RL Anal. Biochem. 269:105-112(1999).
RN [11]
RP NOMENCLATURE.
RX PubMed=24524803; DOI=10.1016/j.sbi.2014.01.002;
RA Ban N., Beckmann R., Cate J.H.D., Dinman J.D., Dragon F., Ellis S.R.,
RA Lafontaine D.L.J., Lindahl L., Liljas A., Lipton J.M., McAlear M.A.,
RA Moore P.B., Noller H.F., Ortega J., Panse V.G., Ramakrishnan V.,
RA Spahn C.M.T., Steitz T.A., Tchorzewski M., Tollervey D., Warren A.J.,
RA Williamson J.R., Wilson D., Yonath A., Yusupov M.;
RT "A new system for naming ribosomal proteins.";
RL Curr. Opin. Struct. Biol. 24:165-169(2014).
RN [12]
RP 3D-STRUCTURE MODELING, AND SUBUNIT.
RX PubMed=12244297; DOI=10.1038/nsb841;
RA Tung C.-S., Joseph S., Sanbonmatsu K.Y.;
RT "All-atom homology model of the Escherichia coli 30S ribosomal subunit.";
RL Nat. Struct. Biol. 9:750-755(2002).
RN [13]
RP STRUCTURE BY ELECTRON MICROSCOPY (11.50 ANGSTROMS), AND SUBUNIT.
RC STRAIN=MRE-600;
RX PubMed=12809609; DOI=10.1016/s0092-8674(03)00427-6;
RA Gao H., Sengupta J., Valle M., Korostelev A., Eswar N., Stagg S.M.,
RA Van Roey P., Agrawal R.K., Harvey S.C., Sali A., Chapman M.S., Frank J.;
RT "Study of the structural dynamics of the E. coli 70S ribosome using real-
RT space refinement.";
RL Cell 113:789-801(2003).
RN [14]
RP X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES,
RP AND SUBUNIT.
RC STRAIN=MRE-600;
RX PubMed=16272117; DOI=10.1126/science.1117230;
RA Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A.,
RA Holton J.M., Cate J.H.D.;
RT "Structures of the bacterial ribosome at 3.5 A resolution.";
RL Science 310:827-834(2005).
RN [15]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.0 ANGSTROMS) OF 70S RIBOSOME IN COMPLEX
RP WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=27906160; DOI=10.1038/nature20822;
RA Ma C., Kurita D., Li N., Chen Y., Himeno H., Gao N.;
RT "Mechanistic insights into the alternative translation termination by ArfA
RT and RF2.";
RL Nature 541:550-553(2017).
RN [16]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.1 ANGSTROMS) OF 70S RIBOSOME IN COMPLEX
RP WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=27906161; DOI=10.1038/nature20821;
RA Huter P., Mueller C., Beckert B., Arenz S., Berninghausen O., Beckmann R.,
RA Wilson D.N.;
RT "Structural basis for ArfA-RF2-mediated translation termination on mRNAs
RT lacking stop codons.";
RL Nature 541:546-549(2017).
RN [17]
RP STRUCTURE BY ELECTRON MICROSCOPY (2.97 ANGSTROMS) OF 70S RIBOSOME IN
RP COMPLEX WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=27934701; DOI=10.1126/science.aai9127;
RA James N.R., Brown A., Gordiyenko Y., Ramakrishnan V.;
RT "Translational termination without a stop codon.";
RL Science 354:1437-1440(2016).
RN [18]
RP STRUCTURE BY ELECTRON MICROSCOPY (3.52 ANGSTROMS) OF 70S RIBOSOME IN
RP COMPLEX WITH ARFA AND RF2, AND SUBUNIT.
RX PubMed=28077875; DOI=10.1038/nature21053;
RA Zeng F., Chen Y., Remis J., Shekhar M., Phillips J.C., Tajkhorshid E.,
RA Jin H.;
RT "Structural basis of co-translational quality control by ArfA and RF2 bound
RT to ribosome.";
RL Nature 541:554-557(2017).
CC -!- FUNCTION: The C-terminal tail plays a role in the affinity of the 30S P
CC site for different tRNAs. Mutations that decrease this affinity are
CC suppressed in the 70S ribosome. {ECO:0000269|PubMed:15308780}.
CC -!- SUBUNIT: Part of the 30S ribosomal subunit (PubMed:1091515,
CC PubMed:7556101, PubMed:10094780, PubMed:12809609, PubMed:16272117,
CC PubMed:27934701, PubMed:12244297, PubMed:27906160, PubMed:27906161,
CC PubMed:28077875). Contacts 16S rRNA (PubMed:7556101). Cross-links to
CC the P site tRNA and weakly to the A site tRNA (PubMed:8524654,
CC PubMed:15308780). {ECO:0000269|PubMed:10094780,
CC ECO:0000269|PubMed:1091515, ECO:0000269|PubMed:12244297,
CC ECO:0000269|PubMed:12809609, ECO:0000269|PubMed:15308780,
CC ECO:0000269|PubMed:16272117, ECO:0000269|PubMed:27906160,
CC ECO:0000269|PubMed:27906161, ECO:0000269|PubMed:27934701,
CC ECO:0000269|PubMed:28077875, ECO:0000269|PubMed:7556101,
CC ECO:0000269|PubMed:8524654}.
CC -!- MASS SPECTROMETRY: Mass=14723.3; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10094780};
CC -!- SIMILARITY: Belongs to the universal ribosomal protein uS9 family.
CC {ECO:0000305}.
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DR EMBL; X02130; CAA26042.1; -; Genomic_DNA.
DR EMBL; U18997; AAA58032.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76262.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77273.1; -; Genomic_DNA.
DR PIR; H65114; R3EC9.
DR RefSeq; NP_417697.1; NC_000913.3.
DR RefSeq; WP_000829818.1; NZ_STEB01000012.1.
DR PDB; 2YKR; EM; 9.80 A; I=4-130.
DR PDB; 3IY8; EM; 14.10 A; I=4-130.
DR PDB; 3J9Y; EM; 3.90 A; i=1-130.
DR PDB; 3J9Z; EM; 3.60 A; SI=2-130.
DR PDB; 3JA1; EM; 3.60 A; SI=2-130.
DR PDB; 3JBU; EM; 3.64 A; I=1-130.
DR PDB; 3JBV; EM; 3.32 A; I=1-130.
DR PDB; 3JCD; EM; 3.70 A; i=1-130.
DR PDB; 3JCE; EM; 3.20 A; i=1-130.
DR PDB; 3JCJ; EM; 3.70 A; o=1-130.
DR PDB; 3JCN; EM; 4.60 A; j=1-130.
DR PDB; 4A2I; EM; 16.50 A; I=4-130.
DR PDB; 4ADV; EM; 13.50 A; I=2-130.
DR PDB; 4U1U; X-ray; 2.95 A; AI/CI=4-130.
DR PDB; 4U1V; X-ray; 3.00 A; AI/CI=4-130.
DR PDB; 4U20; X-ray; 2.90 A; AI/CI=4-130.
DR PDB; 4U24; X-ray; 2.90 A; AI/CI=4-130.
DR PDB; 4U25; X-ray; 2.90 A; AI/CI=4-130.
DR PDB; 4U26; X-ray; 2.80 A; AI/CI=4-130.
DR PDB; 4U27; X-ray; 2.80 A; AI/CI=4-130.
DR PDB; 4V47; EM; 12.30 A; BI=2-130.
DR PDB; 4V48; EM; 11.50 A; BI=2-130.
DR PDB; 4V4H; X-ray; 3.46 A; AI/CI=1-130.
DR PDB; 4V4Q; X-ray; 3.46 A; AI/CI=2-130.
DR PDB; 4V4V; EM; 15.00 A; AI=5-130.
DR PDB; 4V4W; EM; 15.00 A; AI=5-130.
DR PDB; 4V50; X-ray; 3.22 A; AI/CI=2-130.
DR PDB; 4V52; X-ray; 3.21 A; AI/CI=2-130.
DR PDB; 4V53; X-ray; 3.54 A; AI/CI=2-130.
DR PDB; 4V54; X-ray; 3.30 A; AI/CI=2-130.
DR PDB; 4V55; X-ray; 4.00 A; AI/CI=2-130.
DR PDB; 4V56; X-ray; 3.93 A; AI/CI=2-130.
DR PDB; 4V57; X-ray; 3.50 A; AI/CI=2-130.
DR PDB; 4V5B; X-ray; 3.74 A; BI/DI=2-130.
DR PDB; 4V5H; EM; 5.80 A; AI=4-130.
DR PDB; 4V5Y; X-ray; 4.45 A; AI/CI=2-130.
DR PDB; 4V64; X-ray; 3.50 A; AI/CI=2-130.
DR PDB; 4V65; EM; 9.00 A; AW=1-130.
DR PDB; 4V66; EM; 9.00 A; AW=1-130.
DR PDB; 4V69; EM; 6.70 A; AI=4-130.
DR PDB; 4V6C; X-ray; 3.19 A; AI/CI=1-130.
DR PDB; 4V6D; X-ray; 3.81 A; AI/CI=1-130.
DR PDB; 4V6E; X-ray; 3.71 A; AI/CI=1-130.
DR PDB; 4V6K; EM; 8.25 A; BM=1-130.
DR PDB; 4V6L; EM; 13.20 A; AM=1-130.
DR PDB; 4V6M; EM; 7.10 A; AI=2-130.
DR PDB; 4V6N; EM; 12.10 A; BL=2-130.
DR PDB; 4V6O; EM; 14.70 A; AL=2-130.
DR PDB; 4V6P; EM; 13.50 A; AL=2-130.
DR PDB; 4V6Q; EM; 11.50 A; AL=2-130.
DR PDB; 4V6R; EM; 11.50 A; AL=2-130.
DR PDB; 4V6S; EM; 13.10 A; BK=2-130.
DR PDB; 4V6T; EM; 8.30 A; AI=4-130.
DR PDB; 4V6V; EM; 9.80 A; AI=2-130.
DR PDB; 4V6Y; EM; 12.00 A; AI=4-130.
DR PDB; 4V6Z; EM; 12.00 A; AI=4-130.
DR PDB; 4V70; EM; 17.00 A; AI=4-130.
DR PDB; 4V71; EM; 20.00 A; AI=4-130.
DR PDB; 4V72; EM; 13.00 A; AI=4-130.
DR PDB; 4V73; EM; 15.00 A; AI=4-130.
DR PDB; 4V74; EM; 17.00 A; AI=4-130.
DR PDB; 4V75; EM; 12.00 A; AI=4-130.
DR PDB; 4V76; EM; 17.00 A; AI=4-130.
DR PDB; 4V77; EM; 17.00 A; AI=4-130.
DR PDB; 4V78; EM; 20.00 A; AI=4-130.
DR PDB; 4V79; EM; 15.00 A; AI=4-130.
DR PDB; 4V7A; EM; 9.00 A; AI=4-130.
DR PDB; 4V7B; EM; 6.80 A; AI=1-130.
DR PDB; 4V7C; EM; 7.60 A; AI=2-130.
DR PDB; 4V7D; EM; 7.60 A; BI=2-130.
DR PDB; 4V7I; EM; 9.60 A; BI=1-130.
DR PDB; 4V7S; X-ray; 3.25 A; AI/CI=4-130.
DR PDB; 4V7T; X-ray; 3.19 A; AI/CI=4-130.
DR PDB; 4V7U; X-ray; 3.10 A; AI/CI=4-130.
DR PDB; 4V7V; X-ray; 3.29 A; AI/CI=4-130.
DR PDB; 4V85; X-ray; 3.20 A; AI=1-130.
DR PDB; 4V89; X-ray; 3.70 A; AI=1-130.
DR PDB; 4V9C; X-ray; 3.30 A; AI/CI=1-130.
DR PDB; 4V9D; X-ray; 3.00 A; AI/BI=4-130.
DR PDB; 4V9O; X-ray; 2.90 A; BI/DI/FI/HI=1-130.
DR PDB; 4V9P; X-ray; 2.90 A; BI/DI/FI/HI=1-130.
DR PDB; 4WF1; X-ray; 3.09 A; AI/CI=4-130.
DR PDB; 4WOI; X-ray; 3.00 A; AI/DI=1-130.
DR PDB; 4WWW; X-ray; 3.10 A; QI/XI=4-130.
DR PDB; 4YBB; X-ray; 2.10 A; AI/BI=4-130.
DR PDB; 5AFI; EM; 2.90 A; i=1-130.
DR PDB; 5H5U; EM; 3.00 A; p=2-130.
DR PDB; 5IQR; EM; 3.00 A; n=1-130.
DR PDB; 5IT8; X-ray; 3.12 A; AI/BI=4-130.
DR PDB; 5J5B; X-ray; 2.80 A; AI/BI=4-130.
DR PDB; 5J7L; X-ray; 3.00 A; AI/BI=4-130.
DR PDB; 5J88; X-ray; 3.32 A; AI/BI=4-130.
DR PDB; 5J8A; X-ray; 3.10 A; AI/BI=4-130.
DR PDB; 5J91; X-ray; 2.96 A; AI/BI=4-130.
DR PDB; 5JC9; X-ray; 3.03 A; AI/BI=4-130.
DR PDB; 5JTE; EM; 3.60 A; AI=1-130.
DR PDB; 5JU8; EM; 3.60 A; AI=1-130.
DR PDB; 5KCR; EM; 3.60 A; 1i=1-130.
DR PDB; 5KCS; EM; 3.90 A; 1i=1-130.
DR PDB; 5KPS; EM; 3.90 A; 14=1-130.
DR PDB; 5KPV; EM; 4.10 A; 13=1-130.
DR PDB; 5KPW; EM; 3.90 A; 13=1-130.
DR PDB; 5KPX; EM; 3.90 A; 13=1-130.
DR PDB; 5L3P; EM; 3.70 A; i=1-130.
DR PDB; 5LZA; EM; 3.60 A; i=4-130.
DR PDB; 5LZB; EM; 5.30 A; i=4-130.
DR PDB; 5LZC; EM; 4.80 A; i=4-130.
DR PDB; 5LZD; EM; 3.40 A; i=4-130.
DR PDB; 5LZE; EM; 3.50 A; i=4-130.
DR PDB; 5LZF; EM; 4.60 A; i=4-130.
DR PDB; 5MDV; EM; 2.97 A; n=1-130.
DR PDB; 5MDW; EM; 3.06 A; n=1-130.
DR PDB; 5MDY; EM; 3.35 A; n=1-130.
DR PDB; 5MDZ; EM; 3.10 A; n=1-130.
DR PDB; 5ME0; EM; 13.50 A; I=1-130.
DR PDB; 5ME1; EM; 13.50 A; I=1-130.
DR PDB; 5MGP; EM; 3.10 A; i=4-130.
DR PDB; 5MY1; EM; 7.60 A; I=2-130.
DR PDB; 5NO2; EM; 5.16 A; I=4-130.
DR PDB; 5NO3; EM; 5.16 A; I=4-130.
DR PDB; 5NO4; EM; 5.16 A; I=4-130.
DR PDB; 5NP6; EM; 3.60 A; L=4-130.
DR PDB; 5NWY; EM; 2.93 A; 8=1-130.
DR PDB; 5O2R; EM; 3.40 A; i=4-130.
DR PDB; 5U4I; EM; 3.50 A; i=1-130.
DR PDB; 5U9F; EM; 3.20 A; I=1-130.
DR PDB; 5U9G; EM; 3.20 A; I=1-130.
DR PDB; 5UYK; EM; 3.90 A; I=4-130.
DR PDB; 5UYL; EM; 3.60 A; I=4-130.
DR PDB; 5UYM; EM; 3.20 A; I=4-130.
DR PDB; 5UYN; EM; 4.00 A; I=4-130.
DR PDB; 5UYP; EM; 3.90 A; I=4-130.
DR PDB; 5UYQ; EM; 3.80 A; I=4-130.
DR PDB; 5WDT; EM; 3.00 A; i=4-130.
DR PDB; 5WE4; EM; 3.10 A; i=4-130.
DR PDB; 5WE6; EM; 3.40 A; i=4-130.
DR PDB; 5WFK; EM; 3.40 A; i=4-130.
DR PDB; 6BU8; EM; 3.50 A; I=4-130.
DR PDB; 6BY1; X-ray; 3.94 A; AI/BI=1-130.
DR PDB; 6C4I; EM; 3.24 A; i=1-130.
DR PDB; 6ENF; EM; 3.20 A; i=4-130.
DR PDB; 6ENJ; EM; 3.70 A; i=4-130.
DR PDB; 6ENU; EM; 3.10 A; i=4-130.
DR PDB; 6GWT; EM; 3.80 A; i=4-130.
DR PDB; 6GXM; EM; 3.80 A; i=4-130.
DR PDB; 6GXN; EM; 3.90 A; i=4-130.
DR PDB; 6GXO; EM; 3.90 A; i=4-130.
DR PDB; 6GXP; EM; 4.40 A; i=4-130.
DR PDB; 6H4N; EM; 3.00 A; i=4-130.
DR PDB; 6H58; EM; 7.90 A; i/ii=4-130.
DR PDB; 6HRM; EM; 2.96 A; n=4-130.
DR PDB; 6I7V; X-ray; 2.90 A; AI/BI=4-130.
DR PDB; 6O9J; EM; 3.90 A; i=4-130.
DR PDB; 6O9K; EM; 4.00 A; i=4-130.
DR PDB; 6OFX; EM; 3.30 A; N=4-130.
DR PDB; 6OG7; EM; 3.30 A; N=4-130.
DR PDB; 6ORE; EM; 2.90 A; n=4-130.
DR PDB; 6ORL; EM; 3.50 A; n=4-130.
DR PDB; 6OST; EM; 4.20 A; n=4-130.
DR PDB; 6OT3; EM; 3.90 A; n=4-130.
DR PDB; 6OUO; EM; 3.70 A; n=4-130.
DR PDB; 6Q98; EM; 4.30 A; n=1-130.
DR PDB; 6Q9A; EM; 3.70 A; n=4-130.
DR PDB; 6SZS; EM; 3.06 A; i=1-130.
DR PDB; 6TBV; EM; 2.70 A; S091=1-130.
DR PDB; 6TC3; EM; 2.70 A; S091=1-130.
DR PDB; 6VWL; EM; 3.10 A; h=1-130.
DR PDB; 6VWM; EM; 3.40 A; h=1-130.
DR PDB; 6VWN; EM; 3.40 A; h=1-130.
DR PDB; 6W6K; EM; 3.60 A; I=1-130.
DR PDB; 6W77; EM; 3.60 A; I=1-130.
DR PDB; 6W7M; EM; 3.80 A; I=1-130.
DR PDB; 6W7N; EM; 3.40 A; I=1-130.
DR PDB; 6WD6; EM; 3.70 A; N=4-130.
DR PDB; 6WDB; EM; 4.00 A; N=4-130.
DR PDB; 6WDC; EM; 4.20 A; N=4-130.
DR PDB; 6WDD; EM; 3.20 A; N=4-130.
DR PDB; 6WDE; EM; 3.00 A; N=4-130.
DR PDB; 6WDF; EM; 3.30 A; N=4-130.
DR PDB; 6WDG; EM; 3.30 A; N=4-130.
DR PDB; 6WDH; EM; 4.30 A; N=4-130.
DR PDB; 6WDI; EM; 4.00 A; N=4-130.
DR PDB; 6WDJ; EM; 3.70 A; N=4-130.
DR PDB; 6WDK; EM; 3.60 A; N=4-130.
DR PDB; 6WDL; EM; 3.70 A; N=4-130.
DR PDB; 6WDM; EM; 3.60 A; N=4-130.
DR PDB; 6WNV; EM; 3.50 A; N=4-130.
DR PDB; 6WNW; EM; 3.20 A; N=4-130.
DR PDB; 6XE0; EM; 6.80 A; H=4-130.
DR PDB; 6XZA; EM; 2.66 A; I1=4-130.
DR PDB; 6XZB; EM; 2.54 A; I1=4-130.
DR PDB; 6Y69; EM; 2.86 A; i=4-130.
DR PDB; 6ZTL; EM; 3.50 A; AI=1-130.
DR PDB; 7ABZ; EM; 3.21 A; n=4-130.
DR PDB; 7AC7; EM; 3.08 A; n=4-129.
DR PDB; 7ACJ; EM; 3.20 A; n=4-130.
DR PDB; 7ACR; EM; 3.44 A; n=4-130.
DR PDB; 7AF3; EM; 2.82 A; I=1-130.
DR PDB; 7AF5; EM; 2.96 A; I=1-130.
DR PDB; 7AF8; EM; 2.75 A; I=1-130.
DR PDB; 7AFA; EM; 2.95 A; I=1-130.
DR PDB; 7AFD; EM; 3.44 A; I=1-130.
DR PDB; 7AFH; EM; 3.59 A; I=1-130.
DR PDB; 7AFK; EM; 4.90 A; I=1-130.
DR PDB; 7AFN; EM; 3.86 A; I=1-130.
DR PDB; 7B5K; EM; 2.90 A; i=4-130.
DR PDB; 7BOE; EM; 2.90 A; I=1-130.
DR PDB; 7BOH; EM; 2.82 A; I=1-130.
DR PDB; 7D6Z; EM; 3.40 A; p=1-130.
DR PDB; 7D80; EM; 4.10 A; J=1-130.
DR PDB; 7JSS; EM; 3.70 A; N=4-130.
DR PDB; 7JSW; EM; 3.80 A; N=4-130.
DR PDB; 7JSZ; EM; 3.70 A; N=4-130.
DR PDB; 7JT1; EM; 3.30 A; N=4-130.
DR PDB; 7JT2; EM; 3.50 A; N=4-130.
DR PDB; 7JT3; EM; 3.70 A; N=4-130.
DR PDB; 7K50; EM; 3.40 A; N=4-130.
DR PDB; 7K51; EM; 3.50 A; N=4-130.
DR PDB; 7K52; EM; 3.40 A; N=4-130.
DR PDB; 7K53; EM; 3.20 A; N=4-130.
DR PDB; 7K54; EM; 3.20 A; N=4-130.
DR PDB; 7K55; EM; 3.30 A; N=4-130.
DR PDB; 7LV0; EM; 3.20 A; N=4-130.
DR PDB; 7N1P; EM; 2.33 A; SI=1-130.
DR PDB; 7N2C; EM; 2.72 A; SI=1-130.
DR PDB; 7N2U; EM; 2.53 A; SI=1-130.
DR PDB; 7N2V; EM; 2.54 A; SI=1-130.
DR PDB; 7N30; EM; 2.66 A; SI=1-130.
DR PDB; 7N31; EM; 2.69 A; SI=1-130.
DR PDB; 7NAR; EM; 3.00 A; I=1-130.
DR PDB; 7NAT; EM; 3.59 A; I=1-130.
DR PDB; 7NAU; EM; 3.78 A; I=1-130.
DR PDB; 7NAV; EM; 4.80 A; I=1-130.
DR PDB; 7NBU; EM; 3.11 A; I=4-130.
DR PDB; 7O19; EM; 2.90 A; AI=1-130.
DR PDB; 7O1A; EM; 2.40 A; AI=1-130.
DR PDB; 7O1C; EM; 2.60 A; AI=1-130.
DR PDB; 7OE0; EM; 2.69 A; I=2-130.
DR PDB; 7OE1; EM; 3.05 A; I=2-130.
DR PDB; 7OIZ; EM; 2.90 A; I=1-130.
DR PDB; 7OJ0; EM; 3.50 A; I=1-130.
DR PDB; 7P3K; EM; 2.90 A; I=1-130.
DR PDB; 7PJV; EM; 3.10 A; i=1-130.
DR PDB; 7PJY; EM; 3.10 A; i=1-130.
DR PDB; 7QG8; EM; 3.97 A; 8=1-130.
DR PDB; 7QGH; EM; 4.48 A; 8=1-130.
DR PDB; 7SS9; EM; 3.90 A; N=4-130.
DR PDB; 7SSD; EM; 3.30 A; N=4-130.
DR PDB; 7SSL; EM; 3.80 A; N=4-130.
DR PDB; 7SSN; EM; 3.20 A; N=4-130.
DR PDB; 7SSO; EM; 3.20 A; N=4-130.
DR PDB; 7SSW; EM; 3.80 A; N=4-130.
DR PDB; 7ST2; EM; 2.90 A; N=4-130.
DR PDB; 7ST6; EM; 3.00 A; N=4-130.
DR PDB; 7ST7; EM; 3.20 A; N=4-130.
DR PDBsum; 2YKR; -.
DR PDBsum; 3IY8; -.
DR PDBsum; 3J9Y; -.
DR PDBsum; 3J9Z; -.
DR PDBsum; 3JA1; -.
DR PDBsum; 3JBU; -.
DR PDBsum; 3JBV; -.
DR PDBsum; 3JCD; -.
DR PDBsum; 3JCE; -.
DR PDBsum; 3JCJ; -.
DR PDBsum; 3JCN; -.
DR PDBsum; 4A2I; -.
DR PDBsum; 4ADV; -.
DR PDBsum; 4U1U; -.
DR PDBsum; 4U1V; -.
DR PDBsum; 4U20; -.
DR PDBsum; 4U24; -.
DR PDBsum; 4U25; -.
DR PDBsum; 4U26; -.
DR PDBsum; 4U27; -.
DR PDBsum; 4V47; -.
DR PDBsum; 4V48; -.
DR PDBsum; 4V4H; -.
DR PDBsum; 4V4Q; -.
DR PDBsum; 4V4V; -.
DR PDBsum; 4V4W; -.
DR PDBsum; 4V50; -.
DR PDBsum; 4V52; -.
DR PDBsum; 4V53; -.
DR PDBsum; 4V54; -.
DR PDBsum; 4V55; -.
DR PDBsum; 4V56; -.
DR PDBsum; 4V57; -.
DR PDBsum; 4V5B; -.
DR PDBsum; 4V5H; -.
DR PDBsum; 4V5Y; -.
DR PDBsum; 4V64; -.
DR PDBsum; 4V65; -.
DR PDBsum; 4V66; -.
DR PDBsum; 4V69; -.
DR PDBsum; 4V6C; -.
DR PDBsum; 4V6D; -.
DR PDBsum; 4V6E; -.
DR PDBsum; 4V6K; -.
DR PDBsum; 4V6L; -.
DR PDBsum; 4V6M; -.
DR PDBsum; 4V6N; -.
DR PDBsum; 4V6O; -.
DR PDBsum; 4V6P; -.
DR PDBsum; 4V6Q; -.
DR PDBsum; 4V6R; -.
DR PDBsum; 4V6S; -.
DR PDBsum; 4V6T; -.
DR PDBsum; 4V6V; -.
DR PDBsum; 4V6Y; -.
DR PDBsum; 4V6Z; -.
DR PDBsum; 4V70; -.
DR PDBsum; 4V71; -.
DR PDBsum; 4V72; -.
DR PDBsum; 4V73; -.
DR PDBsum; 4V74; -.
DR PDBsum; 4V75; -.
DR PDBsum; 4V76; -.
DR PDBsum; 4V77; -.
DR PDBsum; 4V78; -.
DR PDBsum; 4V79; -.
DR PDBsum; 4V7A; -.
DR PDBsum; 4V7B; -.
DR PDBsum; 4V7C; -.
DR PDBsum; 4V7D; -.
DR PDBsum; 4V7I; -.
DR PDBsum; 4V7S; -.
DR PDBsum; 4V7T; -.
DR PDBsum; 4V7U; -.
DR PDBsum; 4V7V; -.
DR PDBsum; 4V85; -.
DR PDBsum; 4V89; -.
DR PDBsum; 4V9C; -.
DR PDBsum; 4V9D; -.
DR PDBsum; 4V9O; -.
DR PDBsum; 4V9P; -.
DR PDBsum; 4WF1; -.
DR PDBsum; 4WOI; -.
DR PDBsum; 4WWW; -.
DR PDBsum; 4YBB; -.
DR PDBsum; 5AFI; -.
DR PDBsum; 5H5U; -.
DR PDBsum; 5IQR; -.
DR PDBsum; 5IT8; -.
DR PDBsum; 5J5B; -.
DR PDBsum; 5J7L; -.
DR PDBsum; 5J88; -.
DR PDBsum; 5J8A; -.
DR PDBsum; 5J91; -.
DR PDBsum; 5JC9; -.
DR PDBsum; 5JTE; -.
DR PDBsum; 5JU8; -.
DR PDBsum; 5KCR; -.
DR PDBsum; 5KCS; -.
DR PDBsum; 5KPS; -.
DR PDBsum; 5KPV; -.
DR PDBsum; 5KPW; -.
DR PDBsum; 5KPX; -.
DR PDBsum; 5L3P; -.
DR PDBsum; 5LZA; -.
DR PDBsum; 5LZB; -.
DR PDBsum; 5LZC; -.
DR PDBsum; 5LZD; -.
DR PDBsum; 5LZE; -.
DR PDBsum; 5LZF; -.
DR PDBsum; 5MDV; -.
DR PDBsum; 5MDW; -.
DR PDBsum; 5MDY; -.
DR PDBsum; 5MDZ; -.
DR PDBsum; 5ME0; -.
DR PDBsum; 5ME1; -.
DR PDBsum; 5MGP; -.
DR PDBsum; 5MY1; -.
DR PDBsum; 5NO2; -.
DR PDBsum; 5NO3; -.
DR PDBsum; 5NO4; -.
DR PDBsum; 5NP6; -.
DR PDBsum; 5NWY; -.
DR PDBsum; 5O2R; -.
DR PDBsum; 5U4I; -.
DR PDBsum; 5U9F; -.
DR PDBsum; 5U9G; -.
DR PDBsum; 5UYK; -.
DR PDBsum; 5UYL; -.
DR PDBsum; 5UYM; -.
DR PDBsum; 5UYN; -.
DR PDBsum; 5UYP; -.
DR PDBsum; 5UYQ; -.
DR PDBsum; 5WDT; -.
DR PDBsum; 5WE4; -.
DR PDBsum; 5WE6; -.
DR PDBsum; 5WFK; -.
DR PDBsum; 6BU8; -.
DR PDBsum; 6BY1; -.
DR PDBsum; 6C4I; -.
DR PDBsum; 6ENF; -.
DR PDBsum; 6ENJ; -.
DR PDBsum; 6ENU; -.
DR PDBsum; 6GWT; -.
DR PDBsum; 6GXM; -.
DR PDBsum; 6GXN; -.
DR PDBsum; 6GXO; -.
DR PDBsum; 6GXP; -.
DR PDBsum; 6H4N; -.
DR PDBsum; 6H58; -.
DR PDBsum; 6HRM; -.
DR PDBsum; 6I7V; -.
DR PDBsum; 6O9J; -.
DR PDBsum; 6O9K; -.
DR PDBsum; 6OFX; -.
DR PDBsum; 6OG7; -.
DR PDBsum; 6ORE; -.
DR PDBsum; 6ORL; -.
DR PDBsum; 6OST; -.
DR PDBsum; 6OT3; -.
DR PDBsum; 6OUO; -.
DR PDBsum; 6Q98; -.
DR PDBsum; 6Q9A; -.
DR PDBsum; 6SZS; -.
DR PDBsum; 6TBV; -.
DR PDBsum; 6TC3; -.
DR PDBsum; 6VWL; -.
DR PDBsum; 6VWM; -.
DR PDBsum; 6VWN; -.
DR PDBsum; 6W6K; -.
DR PDBsum; 6W77; -.
DR PDBsum; 6W7M; -.
DR PDBsum; 6W7N; -.
DR PDBsum; 6WD6; -.
DR PDBsum; 6WDB; -.
DR PDBsum; 6WDC; -.
DR PDBsum; 6WDD; -.
DR PDBsum; 6WDE; -.
DR PDBsum; 6WDF; -.
DR PDBsum; 6WDG; -.
DR PDBsum; 6WDH; -.
DR PDBsum; 6WDI; -.
DR PDBsum; 6WDJ; -.
DR PDBsum; 6WDK; -.
DR PDBsum; 6WDL; -.
DR PDBsum; 6WDM; -.
DR PDBsum; 6WNV; -.
DR PDBsum; 6WNW; -.
DR PDBsum; 6XE0; -.
DR PDBsum; 6XZA; -.
DR PDBsum; 6XZB; -.
DR PDBsum; 6Y69; -.
DR PDBsum; 6ZTL; -.
DR PDBsum; 7ABZ; -.
DR PDBsum; 7AC7; -.
DR PDBsum; 7ACJ; -.
DR PDBsum; 7ACR; -.
DR PDBsum; 7AF3; -.
DR PDBsum; 7AF5; -.
DR PDBsum; 7AF8; -.
DR PDBsum; 7AFA; -.
DR PDBsum; 7AFD; -.
DR PDBsum; 7AFH; -.
DR PDBsum; 7AFK; -.
DR PDBsum; 7AFN; -.
DR PDBsum; 7B5K; -.
DR PDBsum; 7BOE; -.
DR PDBsum; 7BOH; -.
DR PDBsum; 7D6Z; -.
DR PDBsum; 7D80; -.
DR PDBsum; 7JSS; -.
DR PDBsum; 7JSW; -.
DR PDBsum; 7JSZ; -.
DR PDBsum; 7JT1; -.
DR PDBsum; 7JT2; -.
DR PDBsum; 7JT3; -.
DR PDBsum; 7K50; -.
DR PDBsum; 7K51; -.
DR PDBsum; 7K52; -.
DR PDBsum; 7K53; -.
DR PDBsum; 7K54; -.
DR PDBsum; 7K55; -.
DR PDBsum; 7LV0; -.
DR PDBsum; 7N1P; -.
DR PDBsum; 7N2C; -.
DR PDBsum; 7N2U; -.
DR PDBsum; 7N2V; -.
DR PDBsum; 7N30; -.
DR PDBsum; 7N31; -.
DR PDBsum; 7NAR; -.
DR PDBsum; 7NAT; -.
DR PDBsum; 7NAU; -.
DR PDBsum; 7NAV; -.
DR PDBsum; 7NBU; -.
DR PDBsum; 7O19; -.
DR PDBsum; 7O1A; -.
DR PDBsum; 7O1C; -.
DR PDBsum; 7OE0; -.
DR PDBsum; 7OE1; -.
DR PDBsum; 7OIZ; -.
DR PDBsum; 7OJ0; -.
DR PDBsum; 7P3K; -.
DR PDBsum; 7PJV; -.
DR PDBsum; 7PJY; -.
DR PDBsum; 7QG8; -.
DR PDBsum; 7QGH; -.
DR PDBsum; 7SS9; -.
DR PDBsum; 7SSD; -.
DR PDBsum; 7SSL; -.
DR PDBsum; 7SSN; -.
DR PDBsum; 7SSO; -.
DR PDBsum; 7SSW; -.
DR PDBsum; 7ST2; -.
DR PDBsum; 7ST6; -.
DR PDBsum; 7ST7; -.
DR AlphaFoldDB; P0A7X3; -.
DR SMR; P0A7X3; -.
DR BioGRID; 853248; 1.
DR ComplexPortal; CPX-3802; 30S small ribosomal subunit.
DR DIP; DIP-35799N; -.
DR IntAct; P0A7X3; 165.
DR STRING; 511145.b3230; -.
DR DrugBank; DB00453; Clomocycline.
DR DrugBank; DB01017; Minocycline.
DR DrugBank; DB00595; Oxytetracycline.
DR DrugBank; DB01301; Rolitetracycline.
DR DrugBank; DB00560; Tigecycline.
DR DrugCentral; P0A7X3; -.
DR jPOST; P0A7X3; -.
DR PaxDb; P0A7X3; -.
DR PRIDE; P0A7X3; -.
DR EnsemblBacteria; AAC76262; AAC76262; b3230.
DR EnsemblBacteria; BAE77273; BAE77273; BAE77273.
DR GeneID; 60373613; -.
DR GeneID; 64293309; -.
DR GeneID; 67415938; -.
DR GeneID; 949000; -.
DR KEGG; ecj:JW3199; -.
DR KEGG; eco:b3230; -.
DR PATRIC; fig|1411691.4.peg.3498; -.
DR EchoBASE; EB0901; -.
DR eggNOG; COG0103; Bacteria.
DR HOGENOM; CLU_046483_2_1_6; -.
DR InParanoid; P0A7X3; -.
DR OMA; QWKINGR; -.
DR PhylomeDB; P0A7X3; -.
DR BioCyc; EcoCyc:EG10908-MON; -.
DR BioCyc; MetaCyc:EG10908-MON; -.
DR EvolutionaryTrace; P0A7X3; -.
DR PRO; PR:P0A7X3; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IDA:ComplexPortal.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IDA:EcoCyc.
DR GO; GO:0015935; C:small ribosomal subunit; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003735; F:structural constituent of ribosome; IDA:CAFA.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0002181; P:cytoplasmic translation; IC:ComplexPortal.
DR Gene3D; 3.30.230.10; -; 1.
DR HAMAP; MF_00532_B; Ribosomal_S9_B; 1.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR000754; Ribosomal_S9.
DR InterPro; IPR023035; Ribosomal_S9_bac/plastid.
DR InterPro; IPR020574; Ribosomal_S9_CS.
DR PANTHER; PTHR21569; PTHR21569; 1.
DR Pfam; PF00380; Ribosomal_S9; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR PROSITE; PS00360; RIBOSOMAL_S9; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Ribonucleoprotein; Ribosomal protein; RNA-binding; tRNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1091515"
FT CHAIN 2..130
FT /note="30S ribosomal protein S9"
FT /id="PRO_0000111354"
FT MUTAGEN 105..130
FT /note="Missing: Cold sensitive for growth at 30 degrees
FT Celsius. 350-fold reduced affinity of the 30S subunit P
FT site for certain tRNAs in vitro."
FT /evidence="ECO:0000269|PubMed:15308780"
FT MUTAGEN 128..130
FT /note="Missing: Very cold sensitive for growth at 30
FT degrees Celsius. Almost no P site binding of certain tRNAs
FT in vitro."
FT /evidence="ECO:0000269|PubMed:15308780"
FT CONFLICT 56
FT /note="D -> N (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="Missing (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 126
FT /note="Q -> E (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 6..8
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 11..14
FT /evidence="ECO:0007829|PDB:7OE1"
FT STRAND 19..25
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 28..30
FT /evidence="ECO:0007829|PDB:7BOH"
FT HELIX 35..38
FT /evidence="ECO:0007829|PDB:7OE0"
FT TURN 39..41
FT /evidence="ECO:0007829|PDB:7OE0"
FT TURN 43..49
FT /evidence="ECO:0007829|PDB:7OE0"
FT HELIX 50..54
FT /evidence="ECO:0007829|PDB:7OE0"
FT HELIX 58..60
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 61..65
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:7OE0"
FT HELIX 72..90
FT /evidence="ECO:0007829|PDB:7OE0"
FT STRAND 91..93
FT /evidence="ECO:0007829|PDB:7BOE"
FT HELIX 95..101
FT /evidence="ECO:0007829|PDB:7OE0"
FT TURN 102..104
FT /evidence="ECO:0007829|PDB:7BOH"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:7OE1"
FT STRAND 119..123
FT /evidence="ECO:0007829|PDB:7OE0"
SQ SEQUENCE 130 AA; 14856 MW; 757D599168812806 CRC64;
MAENQYYGTG RRKSSAARVF IKPGNGKIVI NQRSLEQYFG RETARMVVRQ PLELVDMVEK
LDLYITVKGG GISGQAGAIR HGITRALMEY DESLRSELRK AGFVTRDARQ VERKKVGLRK
ARRRPQFSKR
