RSBS_BACSU
ID RSBS_BACSU Reviewed; 121 AA.
AC P42410;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=RsbT antagonist protein RsbS;
DE AltName: Full=Stressosome protein RsbS;
GN Name=rsbS; Synonyms=ycxS; OrderedLocusNames=BSU04680;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8002610; DOI=10.1128/jb.177.1.123-133.1995;
RA Wise A.A., Price C.W.;
RT "Four additional genes in the sigB operon of Bacillus subtilis that control
RT activity of the general stress factor sigma B in response to environmental
RT signals.";
RL J. Bacteriol. 177:123-133(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, PHOSPHORYLATION AT SER-59, AND MUTAGENESIS OF SER-59.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8824586; DOI=10.1101/gad.10.18.2265;
RA Yang X., Kang C.M., Brody M.S., Price C.W.;
RT "Opposing pairs of serine protein kinases and phosphatases transmit signals
RT of environmental stress to activate a bacterial transcription factor.";
RL Genes Dev. 10:2265-2275(1996).
RN [5]
RP FUNCTION, PHOSPHORYLATION AT SER-59, AND MUTAGENESIS OF SER-59.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=8682789; DOI=10.1128/jb.178.13.3846-3853.1996;
RA Kang C.M., Brody M.S., Akbar S., Yang X., Price C.W.;
RT "Homologous pairs of regulatory proteins control activity of Bacillus
RT subtilis transcription factor sigma(b) in response to environmental
RT stress.";
RL J. Bacteriol. 178:3846-3853(1996).
RN [6]
RP FUNCTION, SUBUNIT, AND REQUIREMENT FOR RSBR PARALOG.
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=15312768; DOI=10.1016/j.jmb.2004.05.043;
RA Kim T.-J., Gaidenko T.A., Price C.W.;
RT "A multicomponent protein complex mediates environmental stress signaling
RT in Bacillus subtilis.";
RL J. Mol. Biol. 341:135-150(2004).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=168;
RX PubMed=17218307; DOI=10.1074/mcp.m600464-mcp200;
RA Macek B., Mijakovic I., Olsen J.V., Gnad F., Kumar C., Jensen P.R.,
RA Mann M.;
RT "The serine/threonine/tyrosine phosphoproteome of the model bacterium
RT Bacillus subtilis.";
RL Mol. Cell. Proteomics 6:697-707(2007).
CC -!- FUNCTION: Negative regulator of sigma-B activity. Non-phosphorylated
CC RsbS binds to RsbT, preventing its association with RsbU. Requires any
CC one of RsbRA, RsbRB, RsbRC or RsbRD to sequester RsbT. When RsbS and
CC the RsbR paralog(s) are phosphorylated, they release RsbT, which can
CC then bind and activate RsbU. {ECO:0000269|PubMed:15312768,
CC ECO:0000269|PubMed:8682789, ECO:0000269|PubMed:8824586}.
CC -!- SUBUNIT: Interacts with RsbRA, RsbRB in the stressosome. The
CC stressosome probably also contains RsbRC and RsbRD.
CC {ECO:0000269|PubMed:15312768}.
CC -!- INTERACTION:
CC P42410; P42411: rsbT; NbExp=2; IntAct=EBI-5247936, EBI-5247957;
CC -!- PTM: Phosphorylated by RsbT. Dephosphorylated by RsbX.
CC {ECO:0000269|PubMed:17218307, ECO:0000269|PubMed:8682789,
CC ECO:0000269|PubMed:8824586}.
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DR EMBL; L35574; AAA85081.1; -; Genomic_DNA.
DR EMBL; AB001488; BAA19305.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12275.1; -; Genomic_DNA.
DR PIR; F69701; F69701.
DR RefSeq; NP_388349.1; NC_000964.3.
DR RefSeq; WP_003225190.1; NZ_JNCM01000031.1.
DR PDB; 6JHK; X-ray; 3.10 A; A/B/C/D/E=1-121.
DR PDBsum; 6JHK; -.
DR AlphaFoldDB; P42410; -.
DR SMR; P42410; -.
DR DIP; DIP-382N; -.
DR IntAct; P42410; 3.
DR STRING; 224308.BSU04680; -.
DR iPTMnet; P42410; -.
DR jPOST; P42410; -.
DR PaxDb; P42410; -.
DR PRIDE; P42410; -.
DR DNASU; 938157; -.
DR EnsemblBacteria; CAB12275; CAB12275; BSU_04680.
DR GeneID; 50134765; -.
DR GeneID; 64302334; -.
DR GeneID; 938157; -.
DR KEGG; bsu:BSU04680; -.
DR PATRIC; fig|224308.179.peg.496; -.
DR eggNOG; COG1366; Bacteria.
DR InParanoid; P42410; -.
DR OMA; MSNLMGA; -.
DR PhylomeDB; P42410; -.
DR BioCyc; BSUB:BSU04680-MON; -.
DR PRO; PR:P42410; -.
DR Proteomes; UP000001570; Chromosome.
DR Gene3D; 3.30.750.24; -; 1.
DR InterPro; IPR002645; STAS_dom.
DR InterPro; IPR036513; STAS_dom_sf.
DR Pfam; PF01740; STAS; 1.
DR SUPFAM; SSF52091; SSF52091; 1.
DR PROSITE; PS50801; STAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Phosphoprotein; Reference proteome.
FT CHAIN 1..121
FT /note="RsbT antagonist protein RsbS"
FT /id="PRO_0000097471"
FT DOMAIN 4..115
FT /note="STAS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17218307,
FT ECO:0000269|PubMed:8682789, ECO:0000269|PubMed:8824586"
FT MUTAGEN 59
FT /note="S->A: Loss of phosphorylation and loss of the
FT ability to respond to salt stress. Interacts strongly with
FT RsbT."
FT /evidence="ECO:0000269|PubMed:8682789,
FT ECO:0000269|PubMed:8824586"
FT MUTAGEN 59
FT /note="S->D: Loss of interaction with RsbT."
FT /evidence="ECO:0000269|PubMed:8682789,
FT ECO:0000269|PubMed:8824586"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:6JHK"
FT STRAND 14..18
FT /evidence="ECO:0007829|PDB:6JHK"
FT HELIX 25..42
FT /evidence="ECO:0007829|PDB:6JHK"
FT STRAND 45..50
FT /evidence="ECO:0007829|PDB:6JHK"
FT HELIX 59..75
FT /evidence="ECO:0007829|PDB:6JHK"
FT STRAND 78..82
FT /evidence="ECO:0007829|PDB:6JHK"
FT HELIX 86..94
FT /evidence="ECO:0007829|PDB:6JHK"
FT STRAND 104..107
FT /evidence="ECO:0007829|PDB:6JHK"
FT HELIX 108..118
FT /evidence="ECO:0007829|PDB:6JHK"
SQ SEQUENCE 121 AA; 13311 MW; 64BF3EF7D4A81CDA CRC64;
MRHPKIPILK LYNCLLVSIQ WELDDQTALT FQEDLLNKIY ETGANGVVID LTSVDMIDSF
IAKVLGDVIT MSKLMGAKVV LTGIQPAVAV TLIELGIALE EIETALDLEQ GLETLKRELG
E
