ID   RSBV_STRCO              Reviewed;         113 AA.
AC   Q9WVX8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Anti-sigma-B factor antagonist;
DE   AltName: Full=Anti-anti-sigma-B factor;
GN   Name=rsbV; Synonyms=bldG; OrderedLocusNames=SCO3549; ORFNames=SCH5.12c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=A3(2) / J1501;
RA   Leskiw B.K., Warawa J., Bignell D.R., Strap J.L., Chater K.F.;
RT   "The bldG gene of Streptomyces coelicolor A3(2) encodes a putative anti-
RT   anti-sigma factor.";
RL   Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [3]
RP   PHOSPHORYLATION AT SER-57, AND MUTAGENESIS OF SER-57.
RX   PubMed=12900026; DOI=10.1016/s0378-1097(03)00504-4;
RA   Bignell D.R., Lau L.H., Colvin K.R., Leskiw B.K.;
RT   "The putative anti-anti-sigma factor BldG is post-translationally modified
RT   by phosphorylation in Streptomyces coelicolor.";
RL   FEMS Microbiol. Lett. 225:93-99(2003).
CC   -!- FUNCTION: Positive regulator of sigma-B activity. Non-phosphorylated
CC       RsbV binds to RsbW, preventing its association with sigma-B. When
CC       phosphorylated, releases RsbW, which is then free to complex with and
CC       inactivate sigma-B (By similarity). {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9WVX8; Q9R3X8: prsH; NbExp=3; IntAct=EBI-6406852, EBI-6412450;
CC       Q9WVX8; Q9WVX8: rsbV; NbExp=2; IntAct=EBI-6406852, EBI-6406852;
CC       Q9WVX8; Q9X914: SCO3548; NbExp=6; IntAct=EBI-6406852, EBI-6406843;
CC   -!- PTM: Phosphorylated by RsbW on a serine residue. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the anti-sigma-factor antagonist family.
CC       {ECO:0000305}.
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DR   EMBL; AF134889; AAD30281.1; -; Genomic_DNA.
DR   EMBL; AL939117; CAB38486.1; -; Genomic_DNA.
DR   PIR; T36670; T36670.
DR   RefSeq; NP_627747.1; NC_003888.3.
DR   RefSeq; WP_003975386.1; NZ_VNID01000003.1.
DR   AlphaFoldDB; Q9WVX8; -.
DR   SMR; Q9WVX8; -.
DR   IntAct; Q9WVX8; 2.
DR   STRING; 100226.SCO3549; -.
DR   iPTMnet; Q9WVX8; -.
DR   GeneID; 1098985; -.
DR   KEGG; sco:SCO3549; -.
DR   PATRIC; fig|100226.15.peg.3605; -.
DR   eggNOG; COG1366; Bacteria.
DR   HOGENOM; CLU_115403_3_3_11; -.
DR   InParanoid; Q9WVX8; -.
DR   OMA; MNLAINI; -.
DR   PhylomeDB; Q9WVX8; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0043856; F:anti-sigma factor antagonist activity; IBA:GO_Central.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   Gene3D; 3.30.750.24; -; 1.
DR   InterPro; IPR003658; Anti-sigma_ant.
DR   InterPro; IPR002645; STAS_dom.
DR   InterPro; IPR036513; STAS_dom_sf.
DR   Pfam; PF01740; STAS; 1.
DR   SUPFAM; SSF52091; SSF52091; 1.
DR   TIGRFAMs; TIGR00377; ant_ant_sig; 1.
DR   PROSITE; PS50801; STAS; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..113
FT                   /note="Anti-sigma-B factor antagonist"
FT                   /id="PRO_0000194198"
FT   DOMAIN          3..112
FT                   /note="STAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT   MOD_RES         57
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:12900026"
FT   MUTAGEN         57
FT                   /note="S->A: Abolished phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:12900026"
SQ   SEQUENCE   113 AA;  12321 MW;  DE2708636F8C9B24 CRC64;
     MDLSLSTRTV GDRTVVEVGG EIDVYTAPKL REQLVELVND GSFHLVVDME GVDFLDSTGL
     GVLVGGLKRV RAHEGSLRLV CNQERILKIF RITGLTKVFP IHTSVEEAVA ATD