RSE1_SCHPO
ID RSE1_SCHPO Reviewed; 1206 AA.
AC Q9UTT2;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Pre-mRNA-splicing factor prp12;
DE AltName: Full=Pre-mRNA-processing protein 12;
DE AltName: Full=Spliceosome-associated protein 130;
GN Name=prp12; Synonyms=sap130; ORFNames=SPAPJ698.03c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11350031; DOI=10.1017/s1355838201001200;
RA Habara Y., Urushiyama S., Shibuya T., Ohshima Y., Tani T.;
RT "Mutation in the prp12+ gene encoding a homolog of SAP130/SF3b130 causes
RT differential inhibition of pre-mRNA splicing and arrest of cell-cycle
RT progression in Schizosaccharomyces pombe.";
RL RNA 7:671-681(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION.
RX PubMed=9003295; DOI=10.1007/s004380050304;
RA Urushiyama S., Tani T., Ohshima Y.;
RT "Isolation of novel pre-mRNA splicing mutants of Schizosaccharomyces
RT pombe.";
RL Mol. Gen. Genet. 253:118-127(1996).
RN [4]
RP IDENTIFICATION IN THE CWF COMPLEX, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11884590; DOI=10.1128/mcb.22.7.2011-2024.2002;
RA Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.;
RT "Proteomics analysis reveals stable multiprotein complexes in both fission
RT and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA
RT splicing factors, and snRNAs.";
RL Mol. Cell. Biol. 22:2011-2024(2002).
CC -!- FUNCTION: Involved in mRNA splicing and G2/M transition.
CC {ECO:0000269|PubMed:11350031, ECO:0000269|PubMed:9003295}.
CC -!- SUBUNIT: Belongs to the 40S cdc5-associated complex (or cwf complex), a
CC spliceosome sub-complex reminiscent of a late-stage spliceosome
CC composed of the U2, U5 and U6 snRNAs and at least brr2, cdc5,
CC cwf2/prp3, cwf3/syf1, cwf4/syf3, cwf5/ecm2, spp42/cwf6, cwf7/spf27,
CC cwf8, cwf9, cwf10, cwf11, cwf12, prp45/cwf13, cwf14, cwf15, cwf16,
CC cwf17, cwf18, cwf19, cwf20, cwf21, cwf22, cwf23, cwf24, cwf25, cwf26,
CC cyp7/cwf27, cwf28, cwf29/ist3, lea1, msl1, prp5/cwf1, prp10,
CC prp12/sap130, prp17, prp22, sap61, sap62, sap114, sap145, slu7, smb1,
CC smd1, smd3, smf1, smg1 and syf2. {ECO:0000269|PubMed:11884590}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11350031}.
CC -!- SIMILARITY: Belongs to the RSE1 family. {ECO:0000305}.
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DR EMBL; AB034966; BAA86918.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB92100.1; -; Genomic_DNA.
DR RefSeq; NP_594414.1; NM_001019844.2.
DR AlphaFoldDB; Q9UTT2; -.
DR SMR; Q9UTT2; -.
DR BioGRID; 279706; 24.
DR IntAct; Q9UTT2; 4.
DR STRING; 4896.SPAPJ698.03c.1; -.
DR MaxQB; Q9UTT2; -.
DR PaxDb; Q9UTT2; -.
DR EnsemblFungi; SPAPJ698.03c.1; SPAPJ698.03c.1:pep; SPAPJ698.03c.
DR GeneID; 2543278; -.
DR KEGG; spo:SPAPJ698.03c; -.
DR PomBase; SPAPJ698.03c; prp12.
DR VEuPathDB; FungiDB:SPAPJ698.03c; -.
DR eggNOG; KOG1898; Eukaryota.
DR HOGENOM; CLU_003246_0_0_1; -.
DR InParanoid; Q9UTT2; -.
DR OMA; FFLVQTE; -.
DR PhylomeDB; Q9UTT2; -.
DR Reactome; R-SPO-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:Q9UTT2; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005634; C:nucleus; IDA:PomBase.
DR GO; GO:0005686; C:U2 snRNP; IDA:PomBase.
DR GO; GO:0071004; C:U2-type prespliceosome; ISO:PomBase.
DR GO; GO:0030620; F:U2 snRNA binding; ISO:PomBase.
DR GO; GO:0045292; P:mRNA cis splicing, via spliceosome; IMP:PomBase.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000245; P:spliceosomal complex assembly; ISO:PomBase.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR004871; Cleavage/polyA-sp_fac_asu_C.
DR InterPro; IPR018846; Cleavage/polyA-sp_fac_asu_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF03178; CPSF_A; 1.
DR Pfam; PF10433; MMS1_N; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; Spliceosome.
FT CHAIN 1..1206
FT /note="Pre-mRNA-splicing factor prp12"
FT /id="PRO_0000218635"
SQ SEQUENCE 1206 AA; 134972 MW; 88AC5252ADB7C47D CRC64;
MDTFPSLFLY SLTIQNSNYV QSSCAASLSG KKAQEIVIAT ESRLLIYKVD ATDGRMNCIL
NQNCFGIIRN VAPLRLTGFK RDYLVVTSDS GRITILEYNV EKNKLVPIYQ ETFGKSGIRR
VVPGEYLAID AKGRAAMIAS VEKNKLVYVL NRDSEANLTI SSPLEAHKAN NICFHLIGLD
TGYANPIFAA LEVDYSEIDH DSTREAFTSS EKVLSYYELD LGLNHVVKRW SKVVDRNSYM
LIPVPGGNDG PSGTLVISNG WISYRHLQKA FHQIPILRRQ AASANAISTP WNQVNSNSAN
DGPLIVSAVL HKMKGSFFYL LQTGDGDLLK LTIEHDGQGN VVELRLKYFD TVPLAVQLNI
LKTGFLFVAT EFGNHQLYQF ENLGIDDDEL EITSLDFQAQ DNEVGTKNVH FGVRGLQNLS
LVEEIPSLYS LTDTLLMKAP SSGEANQLYT VCGRGSNSSL RQLRRGLETT EIVASELPGA
PIAIWTLKLN QTDVYDSYII LSFTNGTLVL SIGETVEEIS DSGFLSSVST LNARQMGRDS
LVQIHPKGIR YIRANKQTSE WKLPQDVYVV QSAINDMQIV VALSNGELVY FEMSDDVEGG
QLNEYQERKT LTANVTSLAL GPVQEGSRRS NFMCLACDDA TVRVLSLDLY TTLENLSVQA
LSSPANSLCI IPMNVNGVST LYLHIGLMNG VYLRTVIDVT SGQLLDTRTR FLGPRAVKIY
PITMKNQNTV LAVSSRTFLA YSYQQNLQLS PIAYSAIDHA SSFASEQCPE GIVAIQKNTL
KIFTVDSLQD DLKSDIYPLI CTPRKIVKHP NFPVLYILQS ERNFDSFKYA QENGDVGSSY
TKEKQNEHTS KSWVSFISVF DMISKKIIHE SPLGDNEAAF SMTAAFFKNR DEFFLVAGSA
TNMDLECRTC SHGNFRVYRF HDEGKKLELI SHTEIDGIPM ALTPFQGRML AGVGRFLRIY
DLGNKKMLRK GELSAVPLFI THITVQASRI VVADSQYSVR FVVYKPEDNH LLTFADDTIH
RWTTTNVLVD YDTLAGGDKF GNIWLLRCPE HVSKLADEEN SESKLIHEKP FLNSTPHKLD
LMAHFFTNDI PTSLQKVQLV EGAREVLLWT GLLGTVGVFT PFINQEDVRF FQQLEFLLRK
ECPPLAGRDH LAYRSYYAPV KCVIDGDLCE MYYSLPHPVQ EMIANELDRT IAEVSKKIED
FRVRSF
