ID   RSFA_MYCTU              Reviewed;         128 AA.
AC   P9WGE3; L0T9F0; Q11035;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Anti-sigma-F factor antagonist RsfA;
DE   AltName: Full=Anti-anti-sigma F factor RsfA;
GN   Name=rsfA; OrderedLocusNames=Rv1365c; ORFNames=MTCY02B10.29c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION AS AN ANTI-SIGMA-F FACTOR ANTAGONIST, INTERACTION WITH RSBW,
RP   POSSIBLE DISULFIDE BOND, AND MUTAGENESIS OF CYS-73 AND CYS-109.
RX   PubMed=12354223; DOI=10.1046/j.1365-2958.2002.03135.x;
RA   Beaucher J., Rodrigue S., Jacques P.E., Smith I., Brzezinski R.,
RA   Gaudreau L.;
RT   "Novel Mycobacterium tuberculosis anti-sigma factor antagonists control
RT   sigmaF activity by distinct mechanisms.";
RL   Mol. Microbiol. 45:1527-1540(2002).
RN   [3]
RP   INTERACTION WITH RSBW.
RX   PubMed=19130906; DOI=10.1016/j.bbapap.2008.11.007;
RA   Malik S.S., Luthra A., Ramachandran R.;
RT   "Interactions of the M. tuberculosis UsfX with the cognate sigma factor
RT   SigF and the anti-anti sigma factor RsfA.";
RL   Biochim. Biophys. Acta 1794:541-553(2009).
CC   -!- FUNCTION: Positive, redox-sensitive regulator of sigma-F (SigF)
CC       activity. When reduced binds to anti-sigma-F factor RsbW (UsfX)
CC       preventing its binding to SigF, thus activating transcription.
CC       {ECO:0000269|PubMed:12354223}.
CC   -!- SUBUNIT: Monomer. Interacts with anti-sigma-F factor RsbW (UsfX) under
CC       reducing conditions, with a possible 2:1 RbsW:RsfA stoichiometry.
CC       {ECO:0000269|PubMed:12354223, ECO:0000269|PubMed:19130906}.
CC   -!- SIMILARITY: Belongs to the anti-sigma-factor antagonist family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP44123.1; -; Genomic_DNA.
DR   PIR; D70742; D70742.
DR   RefSeq; NP_215881.1; NC_000962.3.
DR   RefSeq; WP_003898845.1; NZ_NVQJ01000031.1.
DR   AlphaFoldDB; P9WGE3; -.
DR   SMR; P9WGE3; -.
DR   IntAct; P9WGE3; 4.
DR   STRING; 83332.Rv1365c; -.
DR   PaxDb; P9WGE3; -.
DR   DNASU; 886794; -.
DR   GeneID; 45425345; -.
DR   GeneID; 886794; -.
DR   KEGG; mtu:Rv1365c; -.
DR   TubercuList; Rv1365c; -.
DR   eggNOG; COG1366; Bacteria.
DR   OMA; ACNESTW; -.
DR   PhylomeDB; P9WGE3; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0043856; F:anti-sigma factor antagonist activity; IDA:MTBBASE.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MTBBASE.
DR   Gene3D; 3.30.750.24; -; 1.
DR   InterPro; IPR003658; Anti-sigma_ant.
DR   InterPro; IPR002645; STAS_dom.
DR   InterPro; IPR036513; STAS_dom_sf.
DR   Pfam; PF01740; STAS; 1.
DR   SUPFAM; SSF52091; SSF52091; 1.
DR   TIGRFAMs; TIGR00377; ant_ant_sig; 1.
DR   PROSITE; PS50801; STAS; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..128
FT                   /note="Anti-sigma-F factor antagonist RsfA"
FT                   /id="PRO_0000194209"
FT   DOMAIN          17..128
FT                   /note="STAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT   DISULFID        73..109
FT                   /evidence="ECO:0000305"
FT   MUTAGEN         73
FT                   /note="C->A,S: Constitutive interaction with RsbW; leads to
FT                   active SigF."
FT                   /evidence="ECO:0000269|PubMed:12354223"
FT   MUTAGEN         109
FT                   /note="C->A,S: Constitutive interaction with RsbW; leads to
FT                   active SigF."
FT                   /evidence="ECO:0000269|PubMed:12354223"
SQ   SEQUENCE   128 AA;  13589 MW;  BB489351860A0005 CRC64;
     MNPTQAGSFT TPVSNALKAT IQHHDSAVII HARGEIDAAN EHTWQDLVTK AAAATTAPEP
     LVVNLNGLDF MGCCAVAVLA HEAERCRRRG VDVRLVSRDR AVARIIHACG YGDVLPVHPT
     TESALSAT