ID   RSFB_MYCTU              Reviewed;         122 AA.
AC   P9WGE1; F2GFD0; O69655; Q7D525;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Anti-sigma-F factor antagonist RsfB;
DE   AltName: Full=Anti-anti-sigma F factor RsfB;
GN   Name=rsfB; OrderedLocusNames=Rv3687c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION AS AN ANTI-SIGMA-F FACTOR ANTAGONIST, INTERACTION WITH RSBW,
RP   PHOSPHORYLATION AT SER-61, AND MUTAGENESIS OF SER-61.
RX   PubMed=12354223; DOI=10.1046/j.1365-2958.2002.03135.x;
RA   Beaucher J., Rodrigue S., Jacques P.E., Smith I., Brzezinski R.,
RA   Gaudreau L.;
RT   "Novel Mycobacterium tuberculosis anti-sigma factor antagonists control
RT   sigmaF activity by distinct mechanisms.";
RL   Mol. Microbiol. 45:1527-1540(2002).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Positive regulator of sigma-F (SigF) activity. Binds to anti-
CC       sigma-F factor RsbW (UsfX) preventing its binding to SigF, thus
CC       activating transcription. {ECO:0000269|PubMed:12354223}.
CC   -!- SUBUNIT: Interacts with anti-sigma-F factor RsbW (UsfX). Its
CC       phosphorylation may prevent this interaction.
CC       {ECO:0000269|PubMed:12354223}.
CC   -!- PTM: Putative phosphorylation on Ser-61 may prevent interaction with
CC       RsbW. {ECO:0000269|PubMed:12354223}.
CC   -!- SIMILARITY: Belongs to the anti-sigma-factor antagonist family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP46511.1; -; Genomic_DNA.
DR   PIR; H70791; H70791.
DR   RefSeq; NP_218204.1; NC_000962.3.
DR   RefSeq; WP_003419762.1; NZ_NVQJ01000028.1.
DR   AlphaFoldDB; P9WGE1; -.
DR   SMR; P9WGE1; -.
DR   IntAct; P9WGE1; 1.
DR   STRING; 83332.Rv3687c; -.
DR   iPTMnet; P9WGE1; -.
DR   PaxDb; P9WGE1; -.
DR   DNASU; 885599; -.
DR   GeneID; 45427684; -.
DR   GeneID; 885599; -.
DR   KEGG; mtu:Rv3687c; -.
DR   TubercuList; Rv3687c; -.
DR   eggNOG; COG1366; Bacteria.
DR   OMA; DKTFPLY; -.
DR   PhylomeDB; P9WGE1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0043856; F:anti-sigma factor antagonist activity; IPI:MTBBASE.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IPI:MTBBASE.
DR   Gene3D; 3.30.750.24; -; 1.
DR   InterPro; IPR003658; Anti-sigma_ant.
DR   InterPro; IPR002645; STAS_dom.
DR   InterPro; IPR036513; STAS_dom_sf.
DR   Pfam; PF01740; STAS; 1.
DR   SUPFAM; SSF52091; SSF52091; 1.
DR   TIGRFAMs; TIGR00377; ant_ant_sig; 1.
DR   PROSITE; PS50801; STAS; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..122
FT                   /note="Anti-sigma-F factor antagonist RsfB"
FT                   /id="PRO_0000422956"
FT   DOMAIN          7..115
FT                   /note="STAS"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00198"
FT   MOD_RES         61
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000305|PubMed:12354223"
FT   MUTAGEN         61
FT                   /note="S->A: Still interacts with RsbW."
FT                   /evidence="ECO:0000269|PubMed:12354223"
FT   MUTAGEN         61
FT                   /note="S->E: No longer interacts with RsbW."
FT                   /evidence="ECO:0000269|PubMed:12354223"
SQ   SEQUENCE   122 AA;  12651 MW;  3F748FC41C6CCBB7 CRC64;
     MSAPDSITVT VADHNGVAVL SIGGEIDLIT AAALEEAIGE VVADNPTALV IDLSAVEFLG
     SVGLKILAAT SEKIGQSVKF GVVARGSVTR RPIHLMGLDK TFRLFSTLHD ALTGVRGGRI
     DR