RSGA2_ARATH
ID RSGA2_ARATH Reviewed; 294 AA.
AC F4HTL8; A0A1P8AUE0; O64799; Q1PFF7; Q5Q0E0; Q5Q0E1;
DT 10-MAY-2017, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Small ribosomal subunit biogenesis GTPase RsgA 2, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At1g67460 {ECO:0000312|Araport:AT1G67460};
GN ORFNames=F12B7.1 {ECO:0000312|EMBL:AAG52303.1},
GN T1F15.7 {ECO:0000312|EMBL:AAC18794.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=F4HTL8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HTL8-2; Sequence=VSP_058909, VSP_058910;
CC Name=3;
CC IsoId=F4HTL8-3; Sequence=VSP_058911;
CC -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family.
CC {ECO:0000305}.
CC -!- CAUTION: This protein is shorter compared to other members of the
CC family and lacks the C-terminal part that binds zinc. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC18794.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG52303.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC004393; AAC18794.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC011020; AAG52303.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34649.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60272.1; -; Genomic_DNA.
DR EMBL; AY800610; AAV68846.1; -; mRNA.
DR EMBL; AY800611; AAV68847.1; -; mRNA.
DR EMBL; DQ446406; ABE65750.1; -; mRNA.
DR PIR; T02159; T02159.
DR RefSeq; NP_001322570.1; NM_001334299.1. [F4HTL8-1]
DR RefSeq; NP_176913.3; NM_105413.4. [F4HTL8-3]
DR AlphaFoldDB; F4HTL8; -.
DR SMR; F4HTL8; -.
DR STRING; 3702.AT1G67460.1; -.
DR PaxDb; F4HTL8; -.
DR PRIDE; F4HTL8; -.
DR ProteomicsDB; 228046; -. [F4HTL8-1]
DR EnsemblPlants; AT1G67460.1; AT1G67460.1; AT1G67460. [F4HTL8-3]
DR EnsemblPlants; AT1G67460.2; AT1G67460.2; AT1G67460. [F4HTL8-1]
DR GeneID; 843067; -.
DR Gramene; AT1G67460.1; AT1G67460.1; AT1G67460. [F4HTL8-3]
DR Gramene; AT1G67460.2; AT1G67460.2; AT1G67460. [F4HTL8-1]
DR KEGG; ath:AT1G67460; -.
DR Araport; AT1G67460; -.
DR TAIR; locus:2008788; AT1G67460.
DR eggNOG; ENOG502QRR1; Eukaryota.
DR HOGENOM; CLU_931723_0_0_1; -.
DR InParanoid; F4HTL8; -.
DR OrthoDB; 1394936at2759; -.
DR PRO; PR:F4HTL8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HTL8; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR030378; G_CP_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004881; Ribosome_biogen_GTPase_RsgA.
DR InterPro; IPR010914; RsgA_GTPase_dom.
DR PANTHER; PTHR32120; PTHR32120; 1.
DR Pfam; PF03193; RsgA_GTPase; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00157; TIGR00157; 1.
DR PROSITE; PS50936; ENGC_GTPASE; 1.
DR PROSITE; PS51721; G_CP; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; GTP-binding; Mitochondrion; Nucleotide-binding;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..68
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 69..294
FT /note="Small ribosomal subunit biogenesis GTPase RsgA 2,
FT mitochondrial"
FT /id="PRO_0000439701"
FT DOMAIN 155..294
FT /note="CP-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT BINDING 255..263
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT VAR_SEQ 126..140
FT /note="RTVLVGDKVLVDKVD -> SWWKLNLLEFESQLL (in isoform 2)"
FT /id="VSP_058909"
FT VAR_SEQ 141..294
FT /note="Missing (in isoform 2)"
FT /id="VSP_058910"
FT VAR_SEQ 285..294
FT /note="VSFFLSYFIL -> AKTRRIKKSGLKIRV (in isoform 3)"
FT /id="VSP_058911"
FT CONFLICT 61
FT /note="F -> L (in Ref. 3; AAV68846/AAV68847)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="A -> V (in Ref. 3; AAV68846/AAV68847)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 294 AA; 32955 MW; 3EBC0B79405C1D8E CRC64;
MQTFSSAAAL TSILRRTTIY HGGFGTGLRI RRSFYFLSAI RQENPNVTKN PHPNKTILRS
FLAPVLPLDE KPNLVELQAI GTIATALADY MRVIVQDVPE SDNGEDDKIG VELLCVVRKL
LKKIGRTVLV GDKVLVDKVD WVDRRAKIIN VFDRVSEVLD PPVANVDHLV ILFSLDQPKI
DPFTLTRFLV EAESIGIRIT VALNKCELVT QEEVESWKIR LRSWNYEPLF CSVGTKVGID
EIAFNLRNQT SVIVGPSGVG KSSLINILRS SYGGDIKHEE VFKPVSFFLS YFIL
