RSGA_AQUAE
ID RSGA_AQUAE Reviewed; 302 AA.
AC O66555;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Small ribosomal subunit biogenesis GTPase RsgA {ECO:0000255|HAMAP-Rule:MF_01820};
DE EC=3.6.1.- {ECO:0000255|HAMAP-Rule:MF_01820};
GN Name=rsgA {ECO:0000255|HAMAP-Rule:MF_01820}; OrderedLocusNames=aq_168;
OS Aquifex aeolicus (strain VF5).
OC Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX NCBI_TaxID=224324;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VF5;
RX PubMed=9537320; DOI=10.1038/32831;
RA Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL Nature 392:353-358(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH GDP AND ZINC IONS.
RG RIKEN structural genomics initiative (RSGI);
RT "Crystal structure of YjeQ from Aquifex aeolicus.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: One of several proteins that assist in the late maturation
CC steps of the functional core of the 30S ribosomal subunit. Helps
CC release RbfA from mature subunits. May play a role in the assembly of
CC ribosomal proteins into the subunit. Circularly permuted GTPase that
CC catalyzes slow GTP hydrolysis, GTPase activity is stimulated by the 30S
CC ribosomal subunit. {ECO:0000255|HAMAP-Rule:MF_01820}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01820, ECO:0000269|Ref.2};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01820,
CC ECO:0000269|Ref.2};
CC -!- SUBUNIT: Monomer. Associates with 30S ribosomal subunit, binds 16S
CC rRNA. {ECO:0000255|HAMAP-Rule:MF_01820}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01820}.
CC -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01820}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC06518.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE000657; AAC06518.1; ALT_FRAME; Genomic_DNA.
DR PIR; H70315; H70315.
DR PDB; 2YV5; X-ray; 1.90 A; A=1-302.
DR PDBsum; 2YV5; -.
DR AlphaFoldDB; O66555; -.
DR SMR; O66555; -.
DR STRING; 224324.aq_168; -.
DR EnsemblBacteria; AAC06518; AAC06518; aq_168.
DR eggNOG; COG1162; Bacteria.
DR HOGENOM; CLU_1575270_0_0_0; -.
DR InParanoid; O66555; -.
DR OMA; CLVAAYD; -.
DR EvolutionaryTrace; O66555; -.
DR Proteomes; UP000000798; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0042274; P:ribosomal small subunit biogenesis; IEA:UniProtKB-UniRule.
DR CDD; cd01854; YjeQ_EngC; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01820; GTPase_RsgA; 1.
DR InterPro; IPR030378; G_CP_dom.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004881; Ribosome_biogen_GTPase_RsgA.
DR InterPro; IPR010914; RsgA_GTPase_dom.
DR PANTHER; PTHR32120; PTHR32120; 1.
DR Pfam; PF03193; RsgA_GTPase; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00157; TIGR00157; 1.
DR PROSITE; PS50936; ENGC_GTPASE; 1.
DR PROSITE; PS51721; G_CP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; GTP-binding; Hydrolase; Metal-binding;
KW Nucleotide-binding; Reference proteome; Ribosome biogenesis; RNA-binding;
KW rRNA-binding; Zinc.
FT CHAIN 1..302
FT /note="Small ribosomal subunit biogenesis GTPase RsgA"
FT /id="PRO_0000171459"
FT DOMAIN 69..229
FT /note="CP-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT BINDING 118..121
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01820,
FT ECO:0000305|Ref.2"
FT BINDING 172..180
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01820,
FT ECO:0000305|Ref.2"
FT BINDING 252
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01820"
FT BINDING 257
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01820,
FT ECO:0000269|Ref.2"
FT BINDING 259
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01820,
FT ECO:0000269|Ref.2"
FT BINDING 265
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01820,
FT ECO:0000269|Ref.2"
FT STRAND 7..15
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 18..23
FT /evidence="ECO:0007829|PDB:2YV5"
FT TURN 24..26
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 29..34
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 36..38
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 49..56
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 59..65
FT /evidence="ECO:0007829|PDB:2YV5"
FT TURN 73..76
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 77..79
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 82..87
FT /evidence="ECO:0007829|PDB:2YV5"
FT TURN 90..93
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 96..108
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 112..117
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 120..122
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 125..140
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 144..147
FT /evidence="ECO:0007829|PDB:2YV5"
FT TURN 150..152
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 156..162
FT /evidence="ECO:0007829|PDB:2YV5"
FT TURN 163..165
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 167..171
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 178..186
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 209..214
FT /evidence="ECO:0007829|PDB:2YV5"
FT TURN 215..217
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 218..223
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 231..233
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 237..243
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 245..247
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 248..253
FT /evidence="ECO:0007829|PDB:2YV5"
FT STRAND 259..261
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 266..272
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 278..287
FT /evidence="ECO:0007829|PDB:2YV5"
FT HELIX 295..298
FT /evidence="ECO:0007829|PDB:2YV5"
SQ SEQUENCE 302 AA; 34643 MW; 51C223BDF55D86E1 CRC64;
MGKKELKRGL VVDREAQMIG VYLFEDGKTY RGIPRGKVLK KTKINAGDYV WGEVVDPNTF
AIEEVEERKN LLIRPKVANV DRVIIVETLK MPEFNNYLLD NMLVVYEYFK VEPVIVFNKI
DLLNEEEKKE LERMDSIYRD AGYDVLKVSA KTGEGIDELV DYLEGFICIL AGPSGVGKSS
ILSRLTGEEL RTQEVSEKTE RGRHTTTGVR LIPFGKGSFV GDTPGFSKVE ATMFVKPREV
RNYFREFLRY QCKYPDCTHT NEPGCAVKEA VKNGEISCER YKSYLKIIKV YLEEIKELCR
ED
