ID   RSGI5_ACET2             Reviewed;         481 AA.
AC   A3DEX6;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Anti-sigma-I factor RsgI5 {ECO:0000305};
GN   Name=rsgI5 {ECO:0000303|PubMed:20487018};
GN   OrderedLocusNames=Cthe_1273 {ECO:0000312|EMBL:ABN52505.1};
OS   Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS   103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Acetivibrio.
OX   NCBI_TaxID=203119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA   Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE.
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RX   PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA   Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA   Borovok I., Lamed R.;
RT   "The unique set of putative membrane-associated anti-sigma factors in
RT   Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT   sensing mechanism involved in gene regulation.";
RL   FEMS Microbiol. Lett. 308:84-93(2010).
CC   -!- FUNCTION: Anti-sigma factor for SigI5. Negatively regulates SigI5
CC       activity through direct interaction. Binding of the polysaccharide
CC       substrate to the extracellular C-terminal sensing domain of RsgI5 may
CC       induce a conformational change in its N-terminal cytoplasmic region,
CC       leading to the release and activation of SigI5.
CC       {ECO:0000250|UniProtKB:A3DBH1}.
CC   -!- SUBUNIT: Interacts (via RsgI N-terminal anti-sigma domain) with SigI5.
CC       {ECO:0000255|PROSITE-ProRule:PRU01196}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000255}.
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DR   EMBL; CP000568; ABN52505.1; -; Genomic_DNA.
DR   RefSeq; WP_003517501.1; NC_009012.1.
DR   AlphaFoldDB; A3DEX6; -.
DR   SMR; A3DEX6; -.
DR   STRING; 203119.Cthe_1273; -.
DR   CAZy; CBM42; Carbohydrate-Binding Module Family 42.
DR   PRIDE; A3DEX6; -.
DR   EnsemblBacteria; ABN52505; ABN52505; Cthe_1273.
DR   KEGG; cth:Cthe_1273; -.
DR   eggNOG; COG2755; Bacteria.
DR   HOGENOM; CLU_530720_0_0_9; -.
DR   OMA; KECVTIN; -.
DR   OrthoDB; 1808279at2; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046556; F:alpha-L-arabinofuranosidase activity; IEA:InterPro.
DR   GO; GO:0046373; P:L-arabinose metabolic process; IEA:InterPro.
DR   InterPro; IPR007934; AbfB_ABD.
DR   InterPro; IPR036195; AbfB_ABD_sf.
DR   InterPro; IPR024449; Anti-sigma_RsgI_N.
DR   Pfam; PF05270; AbfB; 1.
DR   Pfam; PF12791; RsgI_N; 1.
DR   SUPFAM; SSF110221; SSF110221; 1.
DR   PROSITE; PS51849; RSGI_N; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..481
FT                   /note="Anti-sigma-I factor RsgI5"
FT                   /id="PRO_0000436548"
FT   TOPO_DOM        1..50
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72..481
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          3..50
FT                   /note="RsgI N-terminal anti-sigma"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01196"
FT   REGION          255..339
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        269..285
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        297..317
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        318..336
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   481 AA;  54012 MW;  F44ED7240BA089A6 CRC64;
     MKHKGIVLKL TKSKAIISTN DFQCYYIKRS PTIYVGKEVE FTNKDIVTKK SVLIKPALSV
     ACFILLIACV LSLSKIINNI SPKVFAYISV DINPSFEIEI DDMGNVLNLL PLNDDAKVIA
     DKLEIDKINV SNAIDIIINE AIKSNVINEN EKDFILVSST LNIKKEENSQ QYQSEKEKLD
     IIINSLKDSI EKSGKADVYI VQADVNEREA ARSKGISTGR YVLYNKYKDL ENDLSLEDAK
     DADVNVLIKS MLDVASEERN PEESPKMTPT PTPTHTATHT PTDAPTPKPA NTPTSTPAAK
     PSPKTASNSA STSTPAPKPT STPTPTLMPT PTPTPTPADK IAYGQFMKFE SSNYRGYYIR
     VKSFSGRIDP YVNPVEDSMF KIVPGLADPS CISFESKTYP GYYLKHENFR VILKKYEDTD
     LFREDATFRV VPGWADENMI SFQSYNYPYR YIRHRDFELY IENIKTDLDR KDATFIGIKV
     D