RSGI7_ACET2
ID RSGI7_ACET2 Reviewed; 540 AA.
AC A3DIE5;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Anti-sigma-I factor RsgI7 {ECO:0000305};
GN Name=rsgI7 {ECO:0000303|PubMed:20487018};
GN OrderedLocusNames=Cthe_2522 {ECO:0000312|EMBL:ABN53724.1};
OS Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=203119;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA Newcomb M., Richardson P.;
RT "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NOMENCLATURE.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA Borovok I., Lamed R.;
RT "The unique set of putative membrane-associated anti-sigma factors in
RT Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT sensing mechanism involved in gene regulation.";
RL FEMS Microbiol. Lett. 308:84-93(2010).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC B-4536 / VPI 7372;
RX PubMed=24782837; DOI=10.3389/fmicb.2014.00142;
RA Wei H., Fu Y., Magnusson L., Baker J.O., Maness P.C., Xu Q., Yang S.,
RA Bowersox A., Bogorad I., Wang W., Tucker M.P., Himmel M.E., Ding S.Y.;
RT "Comparison of transcriptional profiles of Clostridium thermocellum grown
RT on cellobiose and pretreated yellow poplar using RNA-Seq.";
RL Front. Microbiol. 5:142-142(2014).
CC -!- FUNCTION: Anti-sigma factor for SigI7. Negatively regulates SigI7
CC activity through direct interaction. {ECO:0000250|UniProtKB:A3DBH1}.
CC -!- SUBUNIT: Interacts (via RsgI N-terminal anti-sigma domain) with SigI7.
CC {ECO:0000255|PROSITE-ProRule:PRU01196}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Up-regulated in pretreated yellow poplar (PYP)-grown cells.
CC {ECO:0000269|PubMed:24782837}.
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DR EMBL; CP000568; ABN53724.1; -; Genomic_DNA.
DR RefSeq; WP_020457850.1; NC_009012.1.
DR AlphaFoldDB; A3DIE5; -.
DR SMR; A3DIE5; -.
DR STRING; 203119.Cthe_2522; -.
DR PRIDE; A3DIE5; -.
DR EnsemblBacteria; ABN53724; ABN53724; Cthe_2522.
DR KEGG; cth:Cthe_2522; -.
DR eggNOG; ENOG5032YNU; Bacteria.
DR HOGENOM; CLU_504069_0_0_9; -.
DR OMA; NQEGNNG; -.
DR OrthoDB; 1808279at2; -.
DR Proteomes; UP000002145; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR024449; Anti-sigma_RsgI_N.
DR Pfam; PF12791; RsgI_N; 1.
DR PROSITE; PS51849; RSGI_N; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Coiled coil; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..540
FT /note="Anti-sigma-I factor RsgI7"
FT /id="PRO_0000436550"
FT TOPO_DOM 1..50
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 51..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..540
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT DOMAIN 1..48
FT /note="RsgI N-terminal anti-sigma"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01196"
FT REGION 238..429
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 481..540
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 451..501
FT /evidence="ECO:0000255"
FT COMPBIAS 238..273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..318
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 336..369
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 370..394
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..417
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 540 AA; 61978 MW; 3019CF99F037ECA8 CRC64;
MRAMVVDMND KYAVVVNKEG QYIKIKRKAE HRLGYQVELP DRVIGFERRT LLKVVSVAAA
LLIVSSISFA VYSYNLPYSY VNVDINPSLE IILNMYNRII DVKALNSEGE MLIEDSYKNS
RLDEGVEKII DSAVAQGFLK NDEENTIMLT VAGKNSRKVL EIKEEVESTA NKVLNDDNVV
SEVIVENIVL ERREEARELG IAPGKLLLIE KLKEVDPKAT TEEYKDKPVN EIVKTIRDIK
KVPNENNRKD DDKKVNNEPN KPLPDRKADV ETSAGVKENT AGPDAGIKPV NKTDNAKPNV
GTDINNKENK TVSNAKIDSG IDKGNKDSKP NSNTKINNDV KKDNKDNKTN SDAKTFNDVS
KDNKNDKADG NAKINNNINR DNKITPINPD NKFSSGGSKD DKDNKHVDSK DKMNNEDNKN
INNGSCPQYN PYWNPYWNPY WNPYWGNPKE KEDMTKQNDE WFKKMQEEQK KQYDEWLKKM
QEEQKKQHDE WVKKMEEMKN TEKMKNPYQE NKIEKPKEAE KENKPDRPPE PGKEILKKRC
