ID   RSGI8_ACET2             Reviewed;         403 AA.
AC   A3DJP2;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   25-MAY-2022, entry version 56.
DE   RecName: Full=Anti-sigma-I factor RsgI8 {ECO:0000305};
GN   Name=rsgI8 {ECO:0000303|PubMed:20487018};
GN   OrderedLocusNames=Cthe_2974 {ECO:0000312|EMBL:ABN54171.1};
OS   Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS   103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Acetivibrio.
OX   NCBI_TaxID=203119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA   Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE.
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RX   PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA   Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA   Borovok I., Lamed R.;
RT   "The unique set of putative membrane-associated anti-sigma factors in
RT   Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT   sensing mechanism involved in gene regulation.";
RL   FEMS Microbiol. Lett. 308:84-93(2010).
CC   -!- FUNCTION: Anti-sigma factor for SigI8. Negatively regulates SigI8
CC       activity through direct interaction. {ECO:0000250|UniProtKB:A3DBH1}.
CC   -!- SUBUNIT: Interacts (via RsgI N-terminal anti-sigma domain) with SigI8.
CC       {ECO:0000255|PROSITE-ProRule:PRU01196}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000568; ABN54171.1; -; Genomic_DNA.
DR   AlphaFoldDB; A3DJP2; -.
DR   SMR; A3DJP2; -.
DR   STRING; 203119.Cthe_2974; -.
DR   EnsemblBacteria; ABN54171; ABN54171; Cthe_2974.
DR   KEGG; cth:Cthe_2974; -.
DR   eggNOG; COG3087; Bacteria.
DR   HOGENOM; CLU_680972_0_0_9; -.
DR   OMA; DICKNAV; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR024449; Anti-sigma_RsgI_N.
DR   Pfam; PF12791; RsgI_N; 1.
DR   PROSITE; PS51849; RSGI_N; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..403
FT                   /note="Anti-sigma-I factor RsgI8"
FT                   /id="PRO_0000436551"
FT   TOPO_DOM        1..59
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        60..80
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        81..403
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          4..51
FT                   /note="RsgI N-terminal anti-sigma"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01196"
FT   REGION          254..403
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        254..311
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        347..403
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   403 AA;  45240 MW;  9AE70649CBF01DBC CRC64;
     MTKQKGTILK LKNNLAIIMT SDCKIVSIKR QPGMYEGLEI SFNKNEIINK KNKLAFYSRI
     AAGIAAIFII MVISFNLFNN NDVYAYVAID SDASIEFELD KNNKIVKVNY YNDNTNTVLD
     ELDLKNKPVD FAIKEVIKKL DLNESVILIS ACLKEQNTKK SSASDNYESE KLSKLIDICK
     NAVEVNVSEN VESKVVEVSY DYKKLAEKNK LSLGRSIVYE KAKEQGIALN IEDIKNKSIG
     ETLQKVKIDD VGVVHNVKKE EPKKPMPEKP EPGKPEPQKP EPGKPDPAKP EPGKPGPEKP
     EPEKPEPAKP EPAKPEPQPQ INDLPKDKTI PEEKTIPNSG VEPMAEPIVE PKDKQQEKPR
     PDSKLKLEEK PTVEPKDSLE EKPVTKPKDD KKEKAKNSIE KMP