ID   RSGI9_ACET2             Reviewed;         707 AA.
AC   A3DC20;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Anti-sigma-I factor RsgI9 {ECO:0000305};
GN   Name=rsgI9 {ECO:0000303|PubMed:20487018};
GN   OrderedLocusNames=Cthe_0260 {ECO:0000312|EMBL:ABN51499.1};
OS   Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC
OS   103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Clostridium thermocellum).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae;
OC   Acetivibrio.
OX   NCBI_TaxID=203119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D.,
RA   Newcomb M., Richardson P.;
RT   "Complete sequence of Clostridium thermocellum ATCC 27405.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NOMENCLATURE.
RC   STRAIN=ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL
RC   B-4536 / VPI 7372;
RX   PubMed=20487018; DOI=10.1111/j.1574-6968.2010.01997.x;
RA   Kahel-Raifer H., Jindou S., Bahari L., Nataf Y., Shoham Y., Bayer E.A.,
RA   Borovok I., Lamed R.;
RT   "The unique set of putative membrane-associated anti-sigma factors in
RT   Clostridium thermocellum suggests a novel extracellular carbohydrate-
RT   sensing mechanism involved in gene regulation.";
RL   FEMS Microbiol. Lett. 308:84-93(2010).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000255}.
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DR   EMBL; CP000568; ABN51499.1; -; Genomic_DNA.
DR   RefSeq; WP_011837788.1; NC_009012.1.
DR   PDB; 7SJY; X-ray; 2.00 A; A/B=387-702.
DR   PDBsum; 7SJY; -.
DR   AlphaFoldDB; A3DC20; -.
DR   SMR; A3DC20; -.
DR   STRING; 203119.Cthe_0260; -.
DR   EnsemblBacteria; ABN51499; ABN51499; Cthe_0260.
DR   KEGG; cth:Cthe_0260; -.
DR   eggNOG; COG0265; Bacteria.
DR   HOGENOM; CLU_390141_0_0_9; -.
DR   OMA; VIVRIHK; -.
DR   OrthoDB; 1808279at2; -.
DR   Proteomes; UP000002145; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR024449; Anti-sigma_RsgI_N.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR001940; Peptidase_S1C.
DR   Pfam; PF12791; RsgI_N; 1.
DR   PRINTS; PR00834; PROTEASES2C.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS51849; RSGI_N; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell membrane; Coiled coil; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..707
FT                   /note="Anti-sigma-I factor RsgI9"
FT                   /id="PRO_0000436552"
FT   TOPO_DOM        1..149
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        150..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        173..707
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          3..50
FT                   /note="RsgI N-terminal anti-sigma"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01196"
FT   REGION          345..392
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          256..283
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        352..369
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   707 AA;  78800 MW;  8BAF0B00E6F06A74 CRC64;
     MKITGVIVRI HKDRAIIRTD DNRLLAVKRH NDMMVGQIVS FDANEVHKVE SKKYKYAASG
     KRIEKVQKTP KIKNFSRINN IKEFSRVDDI KNFSRVAATK ETSQDSPQES KVENFSRVVD
     FSRVMNFSRV SNSKKNEIKN FSRISNIKNF SRIASIAAAF VLIFLFGRNV MLNNSSDSEY
     AYVSVDVNPS VEFTINSKHK VIVTSAINQD ASEVLDGLEL KEKDLKSALV MVLEKAESLG
     YISDDKNYVL VSMALNDKNK KTRDKREEKI DELKETIEQG IEALDNDTIV HRTVTVDLEE
     RNKALENELS MGRYYLYLEA KEKGMDITID EVKSSKISDL IEKIEDNTEL APTPTPVPPE
     TPEPTPTPTA SEATPSNSPV ESKSPEAVPE LGSREIEILG ESVVLVTAYD ENRKVVSQGS
     GFAVGTGLFA TNYHLVKDGV VVKITAGDGK VYDVDGIVKY DKAKDLALLK TRVETGVNPL
     KLGTKKSLTK GSRIVAIGKA NGAKNTVTKG SIKSLKVDGL TDAIELSASI SKESTGGPVF
     DMKGNVVGIT AYGISKQNVN AVIPADYVAD WVKELSKHSF GNIRIVRKTL VFDSDFEFNF
     VVYKIIRALE NEDAATYFGC MTDELYKDET RKNLEVLFTT YDLAYNIESI NVVSKSEEQA
     KVSYVYTINK EAGPNFKNYR IIGECSLIKV DGTWKINDSE EKKEYIQ