ID   RSGI_BACAN              Reviewed;         441 AA.
AC   P0DO99;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Anti-sigma-I factor RsgI {ECO:0000305};
GN   Name=rsgI {ECO:0000303|PubMed:26744224};
GN   OrderedLocusNames=BAS3230 {ECO:0000312|EMBL:AAT55538.1};
OS   Bacillus anthracis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC   Bacillus cereus group.
OX   NCBI_TaxID=1392;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Sterne;
RA   Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K.,
RA   Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R.,
RA   Richardson P., Rubin E., Tice H.;
RT   "Complete genome sequence of Bacillus anthracis Sterne.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Sterne;
RX   PubMed=26744224; DOI=10.1099/mic.0.000236;
RA   Kim J.G., Wilson A.C.;
RT   "Loss of sigmaI affects heat-shock response and virulence gene expression
RT   in Bacillus anthracis.";
RL   Microbiology 162:564-574(2016).
CC   -!- FUNCTION: Anti-sigma factor for SigI. Negatively regulates SigI
CC       activity through direct interaction. Has no direct effect on virulence
CC       gene expression. {ECO:0000269|PubMed:26744224}.
CC   -!- SUBUNIT: Interacts (via RsgI N-terminal anti-sigma domain) with SigI.
CC       {ECO:0000255|PROSITE-ProRule:PRU01196}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Part of the sigI-rsgI operon, which is induced by heat
CC       shock. {ECO:0000269|PubMed:26744224}.
CC   -!- DISRUPTION PHENOTYPE: Loss of rsgI results in a strong increase in sigI
CC       promoter activity in both the exponential and stationary phases.
CC       {ECO:0000269|PubMed:26744224}.
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DR   EMBL; AE017225; AAT55538.1; -; Genomic_DNA.
DR   RefSeq; WP_000981248.1; NZ_WXXJ01000014.1.
DR   RefSeq; YP_029487.1; NC_005945.1.
DR   AlphaFoldDB; P0DO99; -.
DR   SMR; P0DO99; -.
DR   STRING; 260799.BAS3230; -.
DR   GeneID; 45023229; -.
DR   KEGG; bat:BAS3230; -.
DR   PATRIC; fig|260799.14.peg.3460; -.
DR   eggNOG; ENOG5032YNU; Bacteria.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR024449; Anti-sigma_RsgI_N.
DR   Pfam; PF12791; RsgI_N; 1.
DR   PROSITE; PS51849; RSGI_N; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Stress response; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..441
FT                   /note="Anti-sigma-I factor RsgI"
FT                   /id="PRO_0000436481"
FT   TOPO_DOM        1..53
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        54..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        77..441
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          3..51
FT                   /note="RsgI N-terminal anti-sigma"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01196"
FT   REGION          213..441
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        213..233
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        250..277
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        278..431
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   441 AA;  49160 MW;  76851344F1480FFE CRC64;
     MMNKGIVMDI KKHSVVVLTP NGEFITCKRK GDSCMIGEEI SFDEQEQKAS RFSIPYFLKP
     ASLLVACFLC ALLFFYNQPE EKVFAYVSVD INPSLEVSVT KDLRVIDLQA CNDDGRRILK
     ELKQWENKQL QEVIRTIIKQ SQEDKYLTND KQVMLTAIAK DKLLEPKLEK VMKELKKEYE
     LKHITVEYQS STMQVRENAI KAGIGTGVYI KQENEKNKSV TPPATPSNPV ENEEERQSQP
     DSSPDVVPDL SSVKDKKYEK PEYKEQKKIE EQPTKQIKEN NGRGSQQENR GNQQENNGRE
     SQQGNNGNQQ GNNGRESQQG NNGNQQGNNG RGSQGNNGHQ QENNGRGSQG NNGNQQGNNG
     RGSQGNNGHQ QENNGRGSQG NNGNQQGDNG RGSQQGNNGN QQGDNGRGSQ KENVGNEQGN
     NGRGSQQENR GHQQGNEKKN Q